FastQCFastQC Report
Thu 26 May 2016
SRR1546166_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546166_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences7222799
Sequences flagged as poor quality0
Sequence length50
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2570633.5590496149761335No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTATCG207.859248E-444.0000041
CGTTTTT1396500.043.24541
CGGTCTA9600.040.10416431
GTTTTTT1602150.038.5266042
TCACGAC10050.038.0895525
CGACGGT10150.037.93103428
AGGGCGA48050.037.7273676
GTACGGG21700.037.5115173
TGGGCGA25300.036.8695646
TAGGGAC57450.036.8006945
ACGTCGT601.9826984E-1036.66666410
CTATGCG2400.035.751
ATAGGGA76300.035.6671034
ACGGTCT10750.035.60930330
GGGCGAT88900.035.5860527
CTCACGA10800.035.44444324
CACGACG10800.035.44444326
AAGGGAT80650.035.2709245
GGACTAA72500.035.260698
TAGCCGT4250.035.244
Produced by FastQC (version 0.11.5)