##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546166_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	7222799
Sequences flagged as poor quality	0
Sequence length	50
%GC	39
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.90579220050288	33.0	31.0	34.0	30.0	34.0
2	32.291052956063155	33.0	31.0	34.0	31.0	34.0
3	32.21726687950198	33.0	31.0	34.0	30.0	34.0
4	35.997681092883795	37.0	35.0	37.0	35.0	37.0
5	35.95544663502335	37.0	35.0	37.0	35.0	37.0
6	36.019736254601575	37.0	35.0	37.0	35.0	37.0
7	36.20020881101634	37.0	35.0	37.0	35.0	37.0
8	36.0998484105677	37.0	35.0	37.0	35.0	37.0
9	37.84267802551338	39.0	38.0	39.0	35.0	39.0
10	37.576739017657836	39.0	37.0	39.0	35.0	39.0
11	37.491361589876725	39.0	37.0	39.0	35.0	39.0
12	37.35339748482548	39.0	37.0	39.0	35.0	39.0
13	37.29477381275597	39.0	37.0	39.0	34.0	39.0
14	38.61498734770274	40.0	38.0	41.0	34.0	41.0
15	38.64636036528221	40.0	38.0	41.0	34.0	41.0
16	38.63156873118025	40.0	38.0	41.0	34.0	41.0
17	38.52555415151384	40.0	38.0	41.0	34.0	41.0
18	38.17635573134459	39.0	37.0	41.0	34.0	41.0
19	37.81806152988613	39.0	37.0	41.0	34.0	41.0
20	37.424344634261594	39.0	35.0	41.0	34.0	41.0
21	37.33511108366715	39.0	35.0	41.0	33.0	41.0
22	37.24841574575175	39.0	35.0	41.0	33.0	41.0
23	37.151113439540545	38.0	35.0	41.0	33.0	41.0
24	37.08017833529633	38.0	35.0	41.0	33.0	41.0
25	37.04651451604842	38.0	35.0	41.0	33.0	41.0
26	37.004333915425306	38.0	35.0	41.0	33.0	41.0
27	36.92310584857754	38.0	35.0	41.0	33.0	41.0
28	36.80674624892649	38.0	35.0	41.0	33.0	41.0
29	36.69300945519874	38.0	35.0	41.0	32.0	41.0
30	36.457655958583366	38.0	35.0	41.0	31.0	41.0
31	36.114286719040635	38.0	35.0	41.0	30.0	41.0
32	35.587702357493264	38.0	35.0	41.0	27.0	41.0
33	34.89767651017286	38.0	35.0	41.0	21.0	41.0
34	34.23600366007693	38.0	34.0	41.0	17.0	41.0
35	33.759565647611126	38.0	33.0	41.0	15.0	41.0
36	33.536360903854586	38.0	33.0	41.0	12.0	41.0
37	33.37145793479785	38.0	33.0	41.0	10.0	41.0
38	33.239742930683796	38.0	33.0	41.0	10.0	41.0
39	33.11188598768982	38.0	33.0	40.0	10.0	41.0
40	33.03075691293638	38.0	33.0	40.0	10.0	41.0
41	32.88261074411734	38.0	32.0	40.0	10.0	41.0
42	32.78715952084504	38.0	32.0	40.0	10.0	41.0
43	32.664680409907575	37.0	32.0	40.0	10.0	41.0
44	32.49352806301269	37.0	31.0	40.0	10.0	41.0
45	32.4252336248039	37.0	31.0	40.0	10.0	41.0
46	32.353357195735335	37.0	31.0	40.0	10.0	41.0
47	32.287083719206365	37.0	31.0	40.0	10.0	41.0
48	32.19704978637783	36.0	31.0	40.0	9.0	41.0
49	32.09590686380723	36.0	31.0	40.0	8.0	41.0
50	31.9447075849681	36.0	30.0	40.0	8.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	4.0
9	11.0
10	10.0
11	17.0
12	26.0
13	43.0
14	87.0
15	218.0
16	508.0
17	1229.0
18	3033.0
19	6374.0
20	11606.0
21	19197.0
22	29586.0
23	45274.0
24	72947.0
25	130106.0
26	222419.0
27	283429.0
28	267574.0
29	221369.0
30	187909.0
31	180225.0
32	192762.0
33	231570.0
34	367880.0
35	569941.0
36	485231.0
37	582763.0
38	1020180.0
39	2089270.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	28.0284416055327	18.324336590288613	24.03559894162914	29.611622862549545
2	43.74347119447738	20.97548332716998	25.430044502138298	9.851000976214346
3	23.73248930227741	21.439043229639925	44.26446035671213	10.564007111370536
4	21.722271379834883	23.090646714660064	43.551371151266984	11.635710754238072
5	19.20164191195131	25.862480736346114	43.05456928816654	11.881308063536034
6	17.40156413047075	32.93393599904967	40.94775446471652	8.716745405763056
7	70.16840701229538	4.1033815284074775	23.78537738624597	1.9428340730511813
8	70.86107477170553	3.7648147207197655	23.51516358132076	1.8589469262539355
9	65.99427728779384	5.654289978164974	25.21468477802027	3.136747956020928
10	29.774731374914353	27.060741964437884	31.64601423907823	11.518512421569532
11	24.016091268772673	22.640281143085943	39.627975248930504	13.715652339210878
12	22.786166415540567	20.290513414536388	42.472924969945865	14.450395199977184
13	18.46714826205187	22.90911044319522	42.96895151034938	15.654789784403524
14	15.860277989183972	24.873335115652534	43.11512752881535	16.151259366348143
15	15.771863511638632	22.381281827169772	46.92308341959952	14.923771241592076
16	17.898545979197262	21.1055160194822	45.02766863649397	15.968269364826574
17	17.689430371799077	21.56227800330592	42.16681095514357	18.581480669751436
18	18.66705691242412	22.149612636320075	42.93964154339613	16.24368890785968
19	20.152076777991468	23.457263589918533	39.7468903675708	16.643769264519197
20	21.74427116136002	21.731796772968483	41.07727489024684	15.446657175424653
21	19.941715116258944	23.027651745535213	41.42989442181625	15.600738716389587
22	19.98863598447084	21.444235122699663	40.58361585307856	17.983513039750935
23	18.305811915851457	23.16528536928689	41.27909415726507	17.249808557596577
24	17.84139362039564	21.71306442280894	44.4263228147426	16.019219142052822
25	18.193085533738373	23.394462451467916	41.75127952473826	16.66117249005545
26	17.276127994147423	24.76617444289949	40.71645909016712	17.241238472785966
27	16.891844837437674	24.3719366965632	40.95165876829744	17.78455969770168
28	16.012268927876853	23.917680666456313	42.94393350832551	17.12611689734132
29	17.84655782335906	22.21213687380751	42.19149667601162	17.749808626821817
30	18.093276581557923	24.84898167594031	40.79586044136075	16.261881301141013
31	19.812277761017576	24.520064866819634	39.22270299921124	16.44495437295154
32	20.87626971206038	23.705837584570748	38.15558206728444	17.262310636084433
33	20.435180322752995	25.058858760987256	37.069576489668336	17.43638442659141
34	18.86756089986721	24.976729935306246	37.86131941370651	18.29438975112003
35	19.686578568779222	25.941757482106315	36.49126882805406	17.880395121060406
36	20.418593955058142	28.14903197500027	34.0163280190962	17.416046050845384
37	19.840770870129436	28.7114039861832	34.55332205700311	16.894503086684264
38	19.32760416010469	28.648768988310486	34.244619018195024	17.779007833389798
39	19.771185658080753	28.640960381148638	33.311130491101856	18.276723469668752
40	20.67709484924058	27.0568238158088	33.9882917965736	18.27778953837702
41	18.369872953684578	27.901247148093145	33.363727829059066	20.365152069163216
42	19.108270907164936	27.925614432853525	32.253839543368166	20.71227511661338
43	19.375258815869028	27.66611669520362	33.1858051151638	19.77281937376355
44	19.558761084172495	28.11714682908939	32.37001334247291	19.954078744265207
45	19.08519121188337	28.984815997233206	32.012174227747444	19.91781856313598
46	19.66023144213206	29.026558817433518	32.76289427408959	18.550315466344834
47	19.29484677615977	28.1625308969556	33.55897069820163	18.983651628683006
48	19.159289909631987	26.47634248163351	34.60474256586678	19.759625042867732
49	19.109821552558778	26.753866471986832	34.35229195772996	19.78402001772443
50	18.799775544079242	27.51982991635237	34.28850782086003	19.391886718708356
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	9097.0
1	16126.0
2	23155.0
3	221681.5
4	420208.0
5	283817.0
6	147426.0
7	143290.0
8	139154.0
9	138783.5
10	138413.0
11	136745.5
12	135078.0
13	129157.5
14	123237.0
15	117235.0
16	111233.0
17	102735.5
18	94238.0
19	90236.5
20	86235.0
21	84406.5
22	82578.0
23	84072.0
24	85566.0
25	88917.0
26	92268.0
27	98710.5
28	105153.0
29	123206.0
30	141259.0
31	167891.5
32	194524.0
33	216364.5
34	238205.0
35	258869.0
36	279533.0
37	287996.0
38	296459.0
39	311422.0
40	326385.0
41	340581.5
42	354778.0
43	370064.0
44	385350.0
45	393507.5
46	401665.0
47	412043.5
48	422422.0
49	417590.0
50	412758.0
51	382017.5
52	351277.0
53	322997.0
54	294717.0
55	281200.0
56	267683.0
57	261849.5
58	256016.0
59	245841.0
60	235666.0
61	211519.0
62	187372.0
63	163293.5
64	139215.0
65	113264.0
66	87313.0
67	71623.5
68	55934.0
69	49945.0
70	43956.0
71	34871.5
72	25787.0
73	20147.0
74	14507.0
75	11354.5
76	8202.0
77	6259.0
78	4316.0
79	3318.0
80	2320.0
81	1692.0
82	1064.0
83	786.5
84	509.0
85	397.5
86	286.0
87	203.5
88	121.0
89	123.0
90	125.0
91	72.5
92	20.0
93	15.0
94	10.0
95	8.0
96	6.0
97	3.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	7222799.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	20.356313438231176
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.8953591348537	16.263749728091284
2	7.3739114580596326	3.0021130581205235
3	2.5512098258046727	1.5579968058232523
4	1.40381309234906	1.1430583726620014
5	0.9094111071753371	0.9256128770935004
6	0.6271260441718002	0.7659584592263506
7	0.5275498607017186	0.7517279223117566
8	0.4214375789450067	0.6863132361323082
9	0.3572749251207088	0.6545520323439951
>10	3.8689519738096556	18.09251294907871
>50	1.1715162833705717	16.98982659555933
>100	0.8681774368851034	31.004551104920612
>500	0.019837570783744474	2.5463722258315644
>1k	0.004216346658214891	1.8379846593788405
>5k	1.382408740398278E-4	0.16069772785899952
>10k+	6.91204370199139E-5	3.616972245566762
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	257063	3.5590496149761335	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.03104059797316802	0.0
2	0.0	0.0	0.0	0.10055658478105234	0.0
3	0.0	0.0	0.0	0.18802959905155883	0.0
4	0.0	0.0	0.0	0.27197212604144183	0.0
5	0.0	0.0	0.0	0.5068672131122575	0.0
6	0.0	0.0	0.0	0.8583514507326038	0.0
7	0.0	0.0	0.0	1.0747495534625842	0.0
8	0.0	0.0	0.0	1.4522763266705885	0.0
9	0.0	0.0	0.0	1.7170074925247123	0.0
10	0.0	0.0	0.0	2.070831543283982	0.0
11	0.0	0.0	0.0	2.3530490049633115	0.0
12	0.0	0.0	0.0	2.5835275216713076	0.0
13	0.0	0.0	0.0	2.6970984517221095	0.0
14	0.0	0.0	0.0	2.758390479923365	0.0
15	0.0	0.0	0.0	2.8150582620394116	0.0
16	0.0	0.0	0.0	2.902268220394891	0.0
17	0.0	0.0	0.0	3.003157086331767	0.0
18	0.0	0.0	0.0	3.140555344264737	0.0
19	0.0	0.0	0.0	3.223764083702177	0.0
20	1.384504815930777E-5	0.0	0.0	3.317744270607558	0.0
21	1.384504815930777E-5	0.0	0.0	3.4277431782332584	0.0
22	1.384504815930777E-5	0.0	0.0	3.551130247429009	0.0
23	1.384504815930777E-5	0.0	0.0	3.679293858239721	0.0
24	1.384504815930777E-5	0.0	0.0	3.7867591220522683	0.0
25	2.769009631861554E-5	0.0	0.0	3.875187444645767	0.0
26	2.769009631861554E-5	0.0	0.0	3.964667990899373	0.0
27	4.153514447792331E-5	0.0	0.0	4.056183759232398	0.0
28	4.153514447792331E-5	0.0	0.0	4.157086470217433	0.0
29	5.538019263723108E-5	0.0	0.0	4.264191762777838	0.0
30	6.922524079653885E-5	0.0	0.0	4.4010915989770725	0.0
31	6.922524079653885E-5	0.0	0.0	4.515673217543504	0.0
32	8.307028895584663E-5	0.0	0.0	4.634934462387781	0.0
33	8.307028895584663E-5	0.0	0.0	4.753171173668269	0.0
34	8.307028895584663E-5	0.0	0.0	4.876239806756356	0.0
35	8.307028895584663E-5	0.0	0.0	5.011755138139661	0.0
36	8.307028895584663E-5	0.0	0.0	5.139946439046691	0.0
37	8.307028895584663E-5	0.0	0.0	5.26755624793103	0.0
38	8.307028895584663E-5	0.0	0.0	5.4013824834388995	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCTATCG	20	7.859248E-4	44.000004	1
CGTTTTT	139650	0.0	43.2454	1
CGGTCTA	960	0.0	40.104164	31
GTTTTTT	160215	0.0	38.526604	2
TCACGAC	1005	0.0	38.08955	25
CGACGGT	1015	0.0	37.931034	28
AGGGCGA	4805	0.0	37.727367	6
GTACGGG	2170	0.0	37.511517	3
TGGGCGA	2530	0.0	36.869564	6
TAGGGAC	5745	0.0	36.800694	5
ACGTCGT	60	1.9826984E-10	36.666664	10
CTATGCG	240	0.0	35.75	1
ATAGGGA	7630	0.0	35.667103	4
ACGGTCT	1075	0.0	35.609303	30
GGGCGAT	8890	0.0	35.586052	7
CTCACGA	1080	0.0	35.444443	24
CACGACG	1080	0.0	35.444443	26
AAGGGAT	8065	0.0	35.270924	5
GGACTAA	7250	0.0	35.26069	8
TAGCCGT	425	0.0	35.2	44
>>END_MODULE