FastQCFastQC Report
Thu 26 May 2016
SRR1546165_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546165_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences572665
Sequences flagged as poor quality0
Sequence length50
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT74011.2923786157701274No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA53160.928291409462775No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA49290.8607126330402592No Hit
AAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA41900.7316668558406747No Hit
GAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA16420.2867295888521212No Hit
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGA12950.22613569888154506No Hit
AAGGAAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA12170.2125151703002628No Hit
GAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA9040.1578584338138353No Hit
GTGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA8190.14301555010346362No Hit
TAAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA8060.14074546200658325No Hit
TAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA7990.13952310687749384No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAGGGAAGCTCGTATGCC7500.1309666209738678No Hit
GGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGA7240.12642644478010706No Hit
AGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG6720.11734609239258553No Hit
AGTGAGGAGAGCTAAAAGGACATGTAAGAAACCAATCAAGATCAAGGAAG6590.11507600429570518No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6550.11437751565051121No Hit
ATTGCTGGGAATATTTTTTAAAATAAATACTATAAGTATCAAATTATTAC6010.10494791894039274No Hit
GGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGA5840.10197934219831838No Hit
GAATCTGTCTCTTATACACATCTGACGCAGGGAAGCTCGTATGCCGTCTT5740.10023312058533349No Hit
ATGAGCGGGGTAGACAGTAATTTGTACACTGTATAGTTTTGTTAAGAATT5740.10023312058533349No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTTCGT207.8531675E-444.00000440
ATGGGCG800.044.0000045
TAATACG207.8531675E-444.0000041
TCGCATC207.8531675E-444.00000435
GATCGTC207.8531675E-444.0000049
CTCGTAG207.8531675E-444.0000041
CGTATAA207.8531675E-444.00000426
TTTACGA207.8531675E-444.00000419
ACCATTG207.8531675E-444.00000444
CATGCGG302.525483E-644.0000042
ACGTTCA207.8531675E-444.00000425
AACCCGT207.8531675E-444.00000441
GCGCGAC207.8531675E-444.0000049
GACGTGA408.300049E-944.0000048
GGCGTAC207.8531675E-444.0000048
TTCACGG207.8531675E-444.0000042
GTTAGCA207.8531675E-444.00000421
CACGAGG800.044.0000042
CGACAGG302.525483E-644.0000042
CGCGACC207.8531675E-444.00000410
Produced by FastQC (version 0.11.5)