##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546165_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	572665
Sequences flagged as poor quality	0
Sequence length	50
%GC	37
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.95553945151179	33.0	31.0	34.0	30.0	34.0
2	32.329578374791545	33.0	31.0	34.0	31.0	34.0
3	32.29339840919211	34.0	31.0	34.0	30.0	34.0
4	36.00120663913457	37.0	35.0	37.0	35.0	37.0
5	35.962035395912096	37.0	35.0	37.0	35.0	37.0
6	36.01551517903137	37.0	35.0	37.0	35.0	37.0
7	36.18862860485624	37.0	35.0	37.0	35.0	37.0
8	36.0856242305711	37.0	35.0	37.0	35.0	37.0
9	37.74235373211214	39.0	38.0	39.0	35.0	39.0
10	37.57855989103577	39.0	37.0	39.0	35.0	39.0
11	37.477926885701066	39.0	37.0	39.0	35.0	39.0
12	37.1926309447932	39.0	37.0	39.0	34.0	39.0
13	37.063962351462024	39.0	37.0	39.0	33.0	39.0
14	38.35730488156252	40.0	37.0	41.0	33.0	41.0
15	38.4206158923629	40.0	37.0	41.0	34.0	41.0
16	38.456605519806516	40.0	37.0	41.0	34.0	41.0
17	38.38217631599626	40.0	37.0	41.0	34.0	41.0
18	38.23236272515345	40.0	37.0	41.0	34.0	41.0
19	38.08058463499603	40.0	36.0	41.0	34.0	41.0
20	37.83570673954232	40.0	35.0	41.0	34.0	41.0
21	37.7886093964185	40.0	35.0	41.0	34.0	41.0
22	37.73478211519824	40.0	35.0	41.0	33.0	41.0
23	37.6845206185117	40.0	35.0	41.0	33.0	41.0
24	37.60220023923236	39.0	35.0	41.0	33.0	41.0
25	37.5996367859045	40.0	35.0	41.0	33.0	41.0
26	37.565960902098084	40.0	35.0	41.0	33.0	41.0
27	37.51839557158199	40.0	35.0	41.0	33.0	41.0
28	37.443522827482035	40.0	35.0	41.0	33.0	41.0
29	37.39120602795701	40.0	35.0	41.0	33.0	41.0
30	37.25011306784944	40.0	35.0	41.0	33.0	41.0
31	37.05838841207338	40.0	35.0	41.0	32.0	41.0
32	36.773256615997134	40.0	35.0	41.0	31.0	41.0
33	36.46023940698314	40.0	35.0	41.0	30.0	41.0
34	36.06705490993862	40.0	35.0	41.0	29.0	41.0
35	35.87306191228729	40.0	35.0	41.0	26.0	41.0
36	35.670491474072975	39.0	35.0	41.0	25.0	41.0
37	35.63747915447949	39.0	35.0	41.0	24.0	41.0
38	35.607424934298415	39.0	35.0	41.0	24.0	41.0
39	35.53339910768076	39.0	35.0	41.0	24.0	41.0
40	35.384254319715716	39.0	35.0	41.0	23.0	41.0
41	35.29547641291156	39.0	35.0	41.0	23.0	41.0
42	35.25514917098129	39.0	35.0	41.0	23.0	41.0
43	35.16703308216846	39.0	35.0	41.0	23.0	41.0
44	35.0592929548689	39.0	35.0	41.0	22.0	41.0
45	34.99164083713864	39.0	35.0	41.0	22.0	41.0
46	34.9563881152157	39.0	35.0	41.0	22.0	41.0
47	34.92543284468232	39.0	35.0	41.0	22.0	41.0
48	34.840466939659315	38.0	35.0	40.0	22.0	41.0
49	34.70283848323191	38.0	34.0	40.0	21.0	41.0
50	34.625989016266054	38.0	34.0	40.0	20.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	1.0
12	3.0
13	1.0
14	5.0
15	11.0
16	22.0
17	62.0
18	138.0
19	271.0
20	509.0
21	889.0
22	1503.0
23	2360.0
24	3765.0
25	6152.0
26	10434.0
27	13054.0
28	12696.0
29	10986.0
30	10538.0
31	11507.0
32	13814.0
33	17907.0
34	30444.0
35	56753.0
36	32626.0
37	42598.0
38	76315.0
39	217300.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	26.875747601128058	26.220914496258718	28.961609317838526	17.941728584774694
2	32.15789335824609	28.788209511669127	28.525752403237497	10.528144726847284
3	28.147346179703664	25.088315158076714	36.45621785860843	10.308120803611185
4	27.31142989356779	25.5294107375167	35.3845616547196	11.774597714195908
5	22.365257174788052	32.72139907275633	33.751844446578716	11.161499305876909
6	18.28259104362935	41.37200632132224	31.798346328132503	8.547056306915911
7	78.3807286982791	6.041053670121275	13.812787580871888	1.7654300507277378
8	79.35546960264728	5.830983210079191	12.879432128731457	1.9341150585420797
9	69.52913134205862	12.120174971405621	15.216575135550453	3.134118550985306
10	31.71679777880611	39.19516645857526	20.971248461142203	8.11678730147643
11	32.7475923969511	22.26607178717051	33.2562667528136	11.730069063064793
12	31.680825613578616	23.057634044336567	32.88187683898964	12.379663503095179
13	17.698829158408493	36.48503051522269	31.331581290981635	14.484559035387182
14	13.251202710135942	37.66425397047139	33.504230221857455	15.580313097535209
15	16.558546445129352	27.421092610863244	42.55978626247457	13.460574681532833
16	16.15499463036854	27.059275492652773	38.65104380396916	18.134686073009526
17	13.598002322474745	30.53530423546052	33.833043751582515	22.033649690482218
18	15.282407690359983	32.48705613229375	37.02635921524801	15.20417696209826
19	23.253036242829577	28.093911798346326	31.819824853972218	16.83322710485188
20	21.676896614949403	29.458933233216626	34.997424323120846	13.866745828713123
21	16.938873512437464	33.04252922738425	34.21459317401971	15.804004086158574
22	18.531078379157098	29.994848646241696	31.227681105009037	20.24639186959217
23	18.74586363755424	29.3232518138877	32.98455466983315	18.94632987872491
24	14.201147267599731	30.161263565959157	41.825150829891825	13.81243833654929
25	12.671631756786253	36.67449556023155	36.36052491421686	14.293347768765333
26	15.101848375577346	35.05731972444623	33.01057337186663	16.830258528109802
27	17.890738913675534	33.23967764749025	31.595784621026258	17.273798817807968
28	13.060689932159288	33.73385836396497	37.51215806798041	15.69329363589533
29	13.416569896885614	31.623374922511417	34.55999580906813	20.400059371534844
30	13.684614914478797	37.61396278801743	33.290841940750695	15.410580356753076
31	22.11589672845381	33.499515423502395	30.41778352090664	13.966804327137158
32	19.78381776431247	32.294273266220216	30.87022954083103	17.051679428636287
33	18.042136327521327	36.24789362017934	29.096243004199664	16.613727048099676
34	15.012616451153814	33.46895654527516	33.393170527271614	18.125256476299406
35	22.804431910453758	29.211493630656665	32.26231741070259	15.721757048186985
36	20.80064260955358	35.79492373377105	29.213239852269652	14.191193804405716
37	15.764714099866413	37.0270577038932	32.55411104223237	14.654117154008015
38	15.303187727554505	35.383863166074406	33.64165786279937	15.671291243571723
39	19.854888983960954	32.79718509075987	31.691826809740427	15.656099115538751
40	17.451564178009832	34.408947639544934	30.921044589768886	17.218443592676348
41	14.795910348982389	34.578680380327064	31.11539905529411	19.510010215396438
42	15.427693328560327	34.6556887534597	29.115451441942497	20.801166476037476
43	20.573109933381645	30.607772432399393	30.10328900840806	18.7158286258109
44	16.532178498773277	34.90836702085861	29.48076100337894	19.078693476989166
45	14.805165323531208	40.81059607274759	27.337972462085165	17.046266141636035
46	17.019025084473473	38.257794696724964	29.857071760977185	14.866108457824382
47	20.367579649533322	31.151895087005492	32.620642085687095	15.859883177774092
48	17.439515248880237	31.458007735761743	33.68915509067256	17.413321924685462
49	16.494634734094106	34.80167288030524	32.33862729519003	16.365065090410624
50	15.777286895479905	35.71285131796076	31.5440964612819	16.96576532527743
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1143.0
1	1932.5
2	2722.0
3	7838.5
4	12955.0
5	9459.5
6	5964.0
7	5979.0
8	5994.0
9	6142.0
10	6290.0
11	6256.5
12	6223.0
13	6211.5
14	6200.0
15	5676.5
16	5153.0
17	5401.0
18	5649.0
19	5238.5
20	4828.0
21	4819.0
22	4810.0
23	5390.0
24	5970.0
25	8834.5
26	11699.0
27	16635.5
28	21572.0
29	27408.0
30	33244.0
31	36439.0
32	39634.0
33	39719.0
34	39804.0
35	42378.5
36	44953.0
37	41091.5
38	37230.0
39	39639.0
40	42048.0
41	40879.5
42	39711.0
43	37805.0
44	35899.0
45	32774.5
46	29650.0
47	28060.0
48	26470.0
49	24697.5
50	22925.0
51	21300.0
52	19675.0
53	16402.0
54	13129.0
55	11297.5
56	9466.0
57	8674.0
58	7882.0
59	7147.5
60	6413.0
61	5915.5
62	5418.0
63	4760.0
64	4102.0
65	3374.5
66	2647.0
67	2194.0
68	1741.0
69	1496.5
70	1252.0
71	1062.0
72	872.0
73	743.5
74	615.0
75	469.5
76	324.0
77	270.0
78	216.0
79	144.0
80	72.0
81	71.0
82	70.0
83	44.0
84	18.0
85	12.0
86	6.0
87	5.5
88	5.0
89	2.5
90	0.0
91	1.0
92	2.0
93	1.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	572665.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	25.316327491639157
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.95350053571156	19.481800211901028
2	7.524017484714494	3.8096098139170254
3	3.123078721348994	2.3719465107552242
4	1.8446639244363479	1.8680046409217155
5	1.2518032309552791	1.5845530274977926
6	1.003733633547941	1.5246509628763558
7	0.8846287688853063	1.5676886135138186
8	0.676144224430606	1.3693990893812469
9	0.6060032487640444	1.3807599036037037
>10	5.018960012715793	24.417000205598075
>50	0.5809690012169194	10.283855683384921
>100	0.5138009522085271	23.53034814036146
>500	0.01384908226977162	2.2542335071961324
>1k	0.003462270567442905	2.326809342951572
>5k	0.001384908226977162	2.2293403461399186
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7401	1.2923786157701274	No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA	5316	0.928291409462775	No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA	4929	0.8607126330402592	No Hit
AAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA	4190	0.7316668558406747	No Hit
GAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA	1642	0.2867295888521212	No Hit
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGA	1295	0.22613569888154506	No Hit
AAGGAAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA	1217	0.2125151703002628	No Hit
GAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA	904	0.1578584338138353	No Hit
GTGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA	819	0.14301555010346362	No Hit
TAAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA	806	0.14074546200658325	No Hit
TAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA	799	0.13952310687749384	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAGGGAAGCTCGTATGCC	750	0.1309666209738678	No Hit
GGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGA	724	0.12642644478010706	No Hit
AGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	672	0.11734609239258553	No Hit
AGTGAGGAGAGCTAAAAGGACATGTAAGAAACCAATCAAGATCAAGGAAG	659	0.11507600429570518	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	655	0.11437751565051121	No Hit
ATTGCTGGGAATATTTTTTAAAATAAATACTATAAGTATCAAATTATTAC	601	0.10494791894039274	No Hit
GGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGA	584	0.10197934219831838	No Hit
GAATCTGTCTCTTATACACATCTGACGCAGGGAAGCTCGTATGCCGTCTT	574	0.10023312058533349	No Hit
ATGAGCGGGGTAGACAGTAATTTGTACACTGTATAGTTTTGTTAAGAATT	574	0.10023312058533349	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.04871958300227882	0.0
2	0.0	0.0	0.0	0.2050064173644277	0.0
3	0.0	0.0	0.0	0.29528607475574725	0.0
4	0.0	0.0	0.0	0.4582085512472388	0.0
5	0.0	0.0	0.0	0.8745077837828399	0.0
6	0.0	0.0	0.0	1.182541276313377	0.0
7	0.0	0.0	0.0	1.3779434748063877	0.0
8	0.0	0.0	0.0	1.798259017051854	0.0
9	0.0	0.0	0.0	1.9643246924467184	0.0
10	0.0	0.0	0.0	2.3835925017243937	0.0
11	0.0	0.0	0.0	2.7665389014519834	0.0
12	0.0	0.0	0.0	3.1789964464390175	0.0
13	1.746221612984904E-4	0.0	0.0	3.3050736468965276	0.0
14	1.746221612984904E-4	0.0	0.0	3.357460295286075	0.0
15	1.746221612984904E-4	0.0	0.0	3.451581640225961	0.0
16	1.746221612984904E-4	0.0	0.0	3.6393004636218382	0.0
17	1.746221612984904E-4	0.0	0.0	3.8668331397937714	0.0
18	1.746221612984904E-4	0.0	0.0	4.146403220032655	0.0
19	1.746221612984904E-4	0.0	0.0	4.313167384072712	0.0
20	1.746221612984904E-4	0.0	0.0	4.472073550854339	0.0
21	1.746221612984904E-4	0.0	0.0	4.6720159255411104	0.0
22	1.746221612984904E-4	0.0	0.0	4.893436826067596	0.0
23	1.746221612984904E-4	0.0	0.0	5.133716920014319	0.0
24	1.746221612984904E-4	0.0	0.0	5.320562632603703	0.0
25	1.746221612984904E-4	0.0	0.0	5.479992665869226	0.0
26	1.746221612984904E-4	0.0	0.0	5.635755633747479	0.0
27	1.746221612984904E-4	0.0	0.0	5.8065361074974025	0.0
28	1.746221612984904E-4	0.0	0.0	5.966140762924223	0.0
29	1.746221612984904E-4	0.0	0.0	6.150017898771533	0.0
30	1.746221612984904E-4	0.0	0.0	6.39326656946033	0.0
31	1.746221612984904E-4	0.0	0.0	6.622021600761353	0.0
32	1.746221612984904E-4	0.0	0.0	6.813058245221901	0.0
33	1.746221612984904E-4	0.0	0.0	7.009682798844001	0.0
34	1.746221612984904E-4	0.0	0.0	7.214165349724533	0.0
35	1.746221612984904E-4	0.0	0.0	7.493910052124715	0.0
36	1.746221612984904E-4	0.0	0.0	7.719172640199767	0.0
37	1.746221612984904E-4	0.0	0.0	7.946006827726507	0.0
38	1.746221612984904E-4	0.0	0.0	8.158696620188069	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCTTCGT	20	7.8531675E-4	44.000004	40
ATGGGCG	80	0.0	44.000004	5
TAATACG	20	7.8531675E-4	44.000004	1
TCGCATC	20	7.8531675E-4	44.000004	35
GATCGTC	20	7.8531675E-4	44.000004	9
CTCGTAG	20	7.8531675E-4	44.000004	1
CGTATAA	20	7.8531675E-4	44.000004	26
TTTACGA	20	7.8531675E-4	44.000004	19
ACCATTG	20	7.8531675E-4	44.000004	44
CATGCGG	30	2.525483E-6	44.000004	2
ACGTTCA	20	7.8531675E-4	44.000004	25
AACCCGT	20	7.8531675E-4	44.000004	41
GCGCGAC	20	7.8531675E-4	44.000004	9
GACGTGA	40	8.300049E-9	44.000004	8
GGCGTAC	20	7.8531675E-4	44.000004	8
TTCACGG	20	7.8531675E-4	44.000004	2
GTTAGCA	20	7.8531675E-4	44.000004	21
CACGAGG	80	0.0	44.000004	2
CGACAGG	30	2.525483E-6	44.000004	2
CGCGACC	20	7.8531675E-4	44.000004	10
>>END_MODULE