FastQCFastQC Report
Thu 26 May 2016
SRR1546164_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546164_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences584154
Sequences flagged as poor quality0
Sequence length50
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT75481.2921250218264362No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA53570.9170526950085081No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA49080.8401894021097176No Hit
AAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA42050.7198444245866673No Hit
GAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA16370.2802343217713137No Hit
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGA12790.21894911273397083No Hit
AAGGAAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA12160.2081642854452764No Hit
GAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA10010.17135892247592244No Hit
GTGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA9000.15406896126706313No Hit
TAAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA8380.1434553217131099No Hit
TAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA8090.13849087740561564No Hit
AGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG7340.12565179730002704No Hit
GGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGA7220.12359754448313287No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6590.11281271719443846No Hit
AGTGAGGAGAGCTAAAAGGACATGTAAGAAACCAATCAAGATCAAGGAAG6390.10938896249961484No Hit
GGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGA6350.1087042115606501No Hit
ATTGCTGGGAATATTTTTTAAAATAAATACTATAAGTATCAAATTATTAC6260.10716352194797946No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAGGGAAGCTCGTATGCC6080.1040821427226382No Hit
GAATCTGTCTCTTATACACATCTGACGCAGGGAAGCTCGTATGCCGTCTT5880.10065838802781459No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTGACG207.853298E-444.00000410
AAATCGG207.853298E-444.0000042
GATCGTA207.853298E-444.00000410
CATGCGG408.300049E-944.0000042
ACGCATG207.853298E-444.0000041
TCACACG207.853298E-444.00000428
TTTCGCG207.853298E-444.0000041
TGAACGG800.044.0000042
ATTCGAA207.853298E-444.00000412
CGACAGG207.853298E-444.0000042
ACGGCAT207.853298E-444.00000423
TGCGATT207.853298E-444.0000042
TTGACGT207.853298E-444.00000411
GTCAACG408.300049E-944.0000041
TCGTATC207.853298E-444.00000422
TCCGTAT207.853298E-444.00000425
CTTTCGT207.853298E-444.00000419
GCGGACT408.300049E-944.0000045
CACACGG207.853298E-444.0000042
CGGTATC207.853298E-444.0000046
Produced by FastQC (version 0.11.5)