##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1546164_1.fastq.gz File type Conventional base calls Encoding Illumina 1.5 Total Sequences 584154 Sequences flagged as poor quality 0 Sequence length 50 %GC 37 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.886410090489836 31.0 31.0 34.0 30.0 34.0 2 32.298073795608694 33.0 31.0 34.0 31.0 34.0 3 32.37651886317649 34.0 31.0 34.0 30.0 34.0 4 36.03262153473228 37.0 35.0 37.0 35.0 37.0 5 35.918285246698645 37.0 35.0 37.0 35.0 37.0 6 36.00077890419307 37.0 35.0 37.0 35.0 37.0 7 36.170550916367944 37.0 35.0 37.0 35.0 37.0 8 36.06525505260599 37.0 35.0 37.0 35.0 37.0 9 37.71109501946405 39.0 38.0 39.0 35.0 39.0 10 37.58547917158831 39.0 37.0 39.0 35.0 39.0 11 37.47594298763682 39.0 37.0 39.0 35.0 39.0 12 37.185509300629626 39.0 37.0 39.0 34.0 39.0 13 37.03515168945175 39.0 37.0 39.0 33.0 39.0 14 38.31690787018492 40.0 37.0 41.0 33.0 41.0 15 38.398646589769136 40.0 37.0 41.0 34.0 41.0 16 38.420045056611784 40.0 37.0 41.0 34.0 41.0 17 38.347115657857344 40.0 37.0 41.0 34.0 41.0 18 38.21213926464597 40.0 37.0 41.0 34.0 41.0 19 38.070186971243885 40.0 36.0 41.0 34.0 41.0 20 37.83389654098063 40.0 35.0 41.0 34.0 41.0 21 37.77248122926488 40.0 35.0 41.0 34.0 41.0 22 37.73280675986127 40.0 35.0 41.0 33.0 41.0 23 37.658798878377965 40.0 35.0 41.0 33.0 41.0 24 37.5862478045173 39.0 35.0 41.0 33.0 41.0 25 37.575921760357716 39.0 35.0 41.0 33.0 41.0 26 37.55922239683371 40.0 35.0 41.0 33.0 41.0 27 37.50759388791312 40.0 35.0 41.0 33.0 41.0 28 37.427755352184526 39.0 35.0 41.0 33.0 41.0 29 37.377600769660056 40.0 35.0 41.0 33.0 41.0 30 37.23663622948743 39.0 35.0 41.0 33.0 41.0 31 37.04946469595347 39.0 35.0 41.0 32.0 41.0 32 36.77135481397029 40.0 35.0 41.0 31.0 41.0 33 36.44946024507236 40.0 35.0 41.0 30.0 41.0 34 36.12584181568559 40.0 35.0 41.0 29.0 41.0 35 35.89411182667584 40.0 35.0 41.0 27.0 41.0 36 35.70905446166593 39.0 35.0 41.0 25.0 41.0 37 35.59074833006365 39.0 35.0 41.0 24.0 41.0 38 35.56715694833897 39.0 35.0 41.0 24.0 41.0 39 35.502031998411375 39.0 35.0 41.0 24.0 41.0 40 35.37379526631676 39.0 35.0 41.0 23.0 41.0 41 35.30235520085457 39.0 35.0 41.0 23.0 41.0 42 35.250601724887616 39.0 35.0 41.0 23.0 41.0 43 35.19605446508969 39.0 35.0 41.0 23.0 41.0 44 35.07634801781722 39.0 35.0 41.0 22.0 41.0 45 35.013986037928355 39.0 35.0 41.0 23.0 41.0 46 34.990233739733014 39.0 35.0 41.0 23.0 41.0 47 34.95446406255885 39.0 35.0 41.0 22.0 41.0 48 34.87617819958436 38.0 35.0 41.0 22.0 41.0 49 34.72202706820462 38.0 34.0 40.0 22.0 41.0 50 34.619672209725515 38.0 34.0 40.0 20.0 41.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 10 1.0 11 3.0 12 0.0 13 4.0 14 1.0 15 9.0 16 21.0 17 53.0 18 129.0 19 263.0 20 561.0 21 990.0 22 1532.0 23 2391.0 24 3760.0 25 6277.0 26 10191.0 27 13268.0 28 12787.0 29 11412.0 30 10671.0 31 12017.0 32 14196.0 33 18536.0 34 31964.0 35 57606.0 36 33922.0 37 43540.0 38 78434.0 39 219615.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 26.978331056536458 26.31001414010689 28.98054280206932 17.731112001287332 2 32.12149535910051 28.804904186224867 28.5198766078808 10.553723846793824 3 28.279700216038922 24.920654484947462 36.37448344101042 10.425161858003197 4 27.328067598612694 25.464346730485452 35.27152086607298 11.936064804828863 5 22.35112658648233 32.79563265851128 33.67690711695888 11.176333638047502 6 18.346874283151358 41.3307107372371 31.74762134642577 8.574793633185768 7 78.45636595829181 6.0016707922910735 13.743978471430479 1.797984777986627 8 79.2943299198499 5.854106964944176 12.867668457290371 1.9838946579155499 9 69.45377417598783 12.130876446964328 15.229203258044969 3.186146119002866 10 31.787508088620463 39.108865128031304 20.932665016416905 8.170961766931322 11 32.66244860088264 22.291210879322918 33.19775264741832 11.84858787237612 12 31.629672997189097 23.03211139528275 32.9478185546962 12.390397052831958 13 17.83502295627523 36.37533937968412 31.271034692906323 14.518602971134323 14 13.149272280939615 37.59334011236763 33.543551871595504 15.71383573509725 15 16.570459159742125 27.45748552607703 42.538953769040354 13.433101545140493 16 16.19641395933264 26.967203853778283 38.670110963889655 18.166271222999413 17 13.632192880644487 30.50428482900057 33.74829240234596 22.115229888008983 18 15.331915898889676 32.40018899125916 37.03424097070293 15.23365413914824 19 23.174197215117932 28.064174857999774 31.845711918432468 16.915916008449827 20 21.729886297106585 29.47835673469667 34.86649068567536 13.92526628252139 21 17.058686579223973 32.9224827699545 34.1201121622038 15.898718488617728 22 18.61615258989924 29.959565457054133 31.17636787559445 20.24791407745218 23 18.7847725086193 29.11629467571907 33.04556675123341 19.053366064428214 24 14.272263820841765 30.081793499659337 41.81089233318611 13.835050346312789 25 12.681587389626708 36.506640372230606 36.34726459118657 14.46450764695611 26 15.119129544606388 34.89422309870343 33.16146084765318 16.825186509037003 27 18.0394211115562 33.14006238081054 31.576604799419332 17.24391170821393 28 13.172040249660192 33.72107355252211 37.35538916107738 15.75149703674031 29 13.40622507078613 31.593210009689226 34.59738356666222 20.40318135286243 30 13.832140154822186 37.36994011853039 33.332819770129106 15.465099956518316 31 22.1061569380677 33.66595110193545 30.374695713801497 13.853196246195354 32 19.73383731002441 32.325551138912004 30.826117770313992 17.114493780749594 33 18.002615748586845 36.28015899916803 29.156181417913768 16.561043834331358 34 15.169629926355036 33.3377842144366 33.26982268374435 18.222763175464003 35 22.649849183605696 29.261290687044855 32.2178740537598 15.870986075589656 36 20.737339811077216 35.77566874488577 29.1546407283011 14.33235071573592 37 15.775463319604077 37.06128863279204 32.50546944812498 14.657778599478904 38 15.411347007809583 35.35506048062668 33.518216086853805 15.715376424709923 39 19.83757707727757 32.78878514912164 31.647818897071662 15.725818876529136 40 17.530479976170668 34.15657514970367 30.960500142085817 17.35244473203984 41 14.866114072658924 34.564344333857164 30.98634949003174 19.58319210345217 42 15.547270069194083 34.6364143701832 29.04764839408786 20.76866716653485 43 20.631374603272427 30.59381601427021 30.12441924560989 18.650390136847474 44 16.613940844366383 34.74015413743636 29.491367002537 19.154538015660254 45 14.876556524478135 40.68704485461026 27.32977947596011 17.1066191449515 46 17.124422669364588 37.92390362815285 29.97668423052825 14.974989471954315 47 20.33932832780397 31.27822457776545 32.504613509451275 15.877833584979303 48 17.4250283315701 31.510697521543978 33.55758926584428 17.506684881041643 49 16.619418851878102 34.6093667080941 32.312027307867446 16.459187132160356 50 15.9089897527022 35.501425993830395 31.586020124830092 17.00356412863731 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1136.0 1 1888.5 2 2641.0 3 7993.5 4 13346.0 5 9724.0 6 6102.0 7 6009.0 8 5916.0 9 6194.0 10 6472.0 11 6329.5 12 6187.0 13 6215.0 14 6243.0 15 5677.0 16 5111.0 17 5350.0 18 5589.0 19 5246.5 20 4904.0 21 4957.5 22 5011.0 23 5537.5 24 6064.0 25 9002.5 26 11941.0 27 16939.5 28 21938.0 29 27846.0 30 33754.0 31 36870.0 32 39986.0 33 40271.0 34 40556.0 35 42875.0 36 45194.0 37 41722.0 38 38250.0 39 40551.0 40 42852.0 41 41547.5 42 40243.0 43 38519.5 44 36796.0 45 33713.5 46 30631.0 47 28767.5 48 26904.0 49 25500.0 50 24096.0 51 22222.5 52 20349.0 53 16967.5 54 13586.0 55 11816.0 56 10046.0 57 9023.5 58 8001.0 59 7282.5 60 6564.0 61 6007.0 62 5450.0 63 4883.0 64 4316.0 65 3516.0 66 2716.0 67 2202.5 68 1689.0 69 1554.0 70 1419.0 71 1128.0 72 837.0 73 745.5 74 654.0 75 476.5 76 299.0 77 257.5 78 216.0 79 135.5 80 55.0 81 62.0 82 69.0 83 38.5 84 8.0 85 9.0 86 10.0 87 7.0 88 4.0 89 3.0 90 2.0 91 1.0 92 0.0 93 0.5 94 1.0 95 0.5 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 50 584154.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 25.50642089310327 #Duplication Level Percentage of deduplicated Percentage of total 1 76.96386860783517 19.63072826272942 2 7.66740297531695 3.911360148909329 3 3.12130864976692 2.3884023647471677 4 1.842832019604094 1.8801619650923824 5 1.2677915656930974 1.6168412639647265 6 0.9917112674191221 1.5177002994735014 7 0.8501352863975381 1.517873590165415 8 0.6645640383731907 1.3560520058533632 9 0.5996047384796028 1.376439374624388 >10 4.94506882499042 24.530564074251995 >50 0.5563263855945203 9.956977130837048 >100 0.5118741562370889 23.657612743992953 >500 0.012123335279299475 1.9950030265771332 >1k 0.004041111759766491 2.447327401758188 >5k 0.0013470372532554972 2.216956347022983 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7548 1.2921250218264362 No Hit AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA 5357 0.9170526950085081 No Hit AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA 4908 0.8401894021097176 No Hit AAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA 4205 0.7198444245866673 No Hit GAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA 1637 0.2802343217713137 No Hit GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGA 1279 0.21894911273397083 No Hit AAGGAAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA 1216 0.2081642854452764 No Hit GAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA 1001 0.17135892247592244 No Hit GTGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA 900 0.15406896126706313 No Hit TAAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA 838 0.1434553217131099 No Hit TAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA 809 0.13849087740561564 No Hit AGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG 734 0.12565179730002704 No Hit GGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGA 722 0.12359754448313287 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 659 0.11281271719443846 No Hit AGTGAGGAGAGCTAAAAGGACATGTAAGAAACCAATCAAGATCAAGGAAG 639 0.10938896249961484 No Hit GGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGA 635 0.1087042115606501 No Hit ATTGCTGGGAATATTTTTTAAAATAAATACTATAAGTATCAAATTATTAC 626 0.10716352194797946 No Hit GAATGATACCTGTCTCTTATACACATCTGACGCAGGGAAGCTCGTATGCC 608 0.1040821427226382 No Hit GAATCTGTCTCTTATACACATCTGACGCAGGGAAGCTCGTATGCCGTCTT 588 0.10065838802781459 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.04331049688951886 0.0 2 0.0 0.0 0.0 0.20765072224105288 0.0 3 0.0 0.0 0.0 0.30197516408344377 0.0 4 0.0 0.0 0.0 0.47196458468143676 0.0 5 0.0 0.0 0.0 0.9105475610883431 0.0 6 0.0 0.0 0.0 1.22313636472574 0.0 7 0.0 0.0 0.0 1.4153801908400867 0.0 8 0.0 0.0 0.0 1.8575581096765579 0.0 9 0.0 0.0 0.0 2.0328543500515277 0.0 10 0.0 0.0 0.0 2.4442184766345862 0.0 11 0.0 0.0 0.0 2.844626588194209 0.0 12 0.0 0.0 0.0 3.2594144694720915 0.0 13 0.0 0.0 0.0 3.4092037373706248 0.0 14 0.0 0.0 0.0 3.4629566860793557 0.0 15 0.0 0.0 0.0 3.566867641067253 0.0 16 0.0 0.0 0.0 3.7599674058553054 0.0 17 0.0 0.0 0.0 4.001855675044594 0.0 18 0.0 0.0 0.0 4.309480034374497 0.0 19 0.0 0.0 0.0 4.476216888012408 0.0 20 0.0 0.0 0.0 4.643467304854542 0.0 21 0.0 0.0 0.0 4.86789442510023 0.0 22 0.0 0.0 0.0 5.097285989653414 0.0 23 0.0 0.0 0.0 5.356464220051562 0.0 24 0.0 0.0 0.0 5.556411494229262 0.0 25 0.0 0.0 0.0 5.721950033723984 0.0 26 0.0 0.0 0.0 5.892110642056718 0.0 27 0.0 0.0 0.0 6.053711863652393 0.0 28 0.0 0.0 0.0 6.233116609661151 0.0 29 0.0 0.0 0.0 6.4245044971017915 0.0 30 0.0 0.0 0.0 6.6776911567839985 0.0 31 0.0 0.0 0.0 6.905199656255029 0.0 32 0.0 0.0 0.0 7.1128503784960815 0.0 33 0.0 0.0 0.0 7.32272654128877 0.0 34 0.0 0.0 0.0 7.539792588940587 0.0 35 0.0 0.0 0.0 7.849471201087384 0.0 36 0.0 0.0 0.0 8.08211533260065 0.0 37 0.0 0.0 0.0 8.314245900909691 0.0 38 0.0 0.0 0.0 8.538159457951156 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTTGACG 20 7.853298E-4 44.000004 10 AAATCGG 20 7.853298E-4 44.000004 2 GATCGTA 20 7.853298E-4 44.000004 10 CATGCGG 40 8.300049E-9 44.000004 2 ACGCATG 20 7.853298E-4 44.000004 1 TCACACG 20 7.853298E-4 44.000004 28 TTTCGCG 20 7.853298E-4 44.000004 1 TGAACGG 80 0.0 44.000004 2 ATTCGAA 20 7.853298E-4 44.000004 12 CGACAGG 20 7.853298E-4 44.000004 2 ACGGCAT 20 7.853298E-4 44.000004 23 TGCGATT 20 7.853298E-4 44.000004 2 TTGACGT 20 7.853298E-4 44.000004 11 GTCAACG 40 8.300049E-9 44.000004 1 TCGTATC 20 7.853298E-4 44.000004 22 TCCGTAT 20 7.853298E-4 44.000004 25 CTTTCGT 20 7.853298E-4 44.000004 19 GCGGACT 40 8.300049E-9 44.000004 5 CACACGG 20 7.853298E-4 44.000004 2 CGGTATC 20 7.853298E-4 44.000004 6 >>END_MODULE