FastQCFastQC Report
Thu 26 May 2016
SRR1546163_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546163_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1349745
Sequences flagged as poor quality0
Sequence length50
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT188721.3981900284868625No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA88240.6537531163293807No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA82640.6122637979766549No Hit
GAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA62870.4657916865778351No Hit
AAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA56560.41904211536253144No Hit
GAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA21670.16054884441135178No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA21380.15840029042522846No Hit
AGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG18860.1397300971665018No Hit
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGA18700.13854468807070966No Hit
GGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGA17090.12661650904430094No Hit
GAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA15800.11705914820947662No Hit
TAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA15780.11691097207250259No Hit
AAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA15200.11261386410025596No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14840.10994669363472359No Hit
AAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA13750.10187109416963944No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTAGCG650.044.0000041
ACGCGAG408.314601E-944.01
TAATACG551.8189894E-1244.01
GGTACGC207.85697E-444.016
ACCGGTT254.4432552E-544.031
GTCGATA254.4432552E-544.030
GTCGAAT254.4432552E-544.012
ATGTGCG551.8189894E-1244.01
ACCGACT254.4432552E-544.025
TAACGTG351.446333E-744.027
CGCGAGG600.044.02
ATCCGTT850.044.022
ATCGTAG351.446333E-744.01
TGTTGCG207.85697E-444.01
ACGATGT207.85697E-444.09
ATATCGT254.4432552E-544.029
CGAGGGT600.044.04
ACTACGG800.044.02
TATTACG600.044.01
GCGATCG254.4432552E-544.09
Produced by FastQC (version 0.11.5)