##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546163_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1349745
Sequences flagged as poor quality	0
Sequence length	50
%GC	37
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.893637687118677	31.0	31.0	34.0	30.0	34.0
2	32.26145383016792	33.0	31.0	34.0	30.0	34.0
3	32.19943396715676	34.0	31.0	34.0	30.0	34.0
4	35.92571633901218	37.0	35.0	37.0	35.0	37.0
5	35.89019036929198	37.0	35.0	37.0	35.0	37.0
6	35.96127564836321	37.0	35.0	37.0	35.0	37.0
7	36.15154566232881	37.0	35.0	37.0	35.0	37.0
8	36.03311366221027	37.0	35.0	37.0	35.0	37.0
9	37.70810856865556	39.0	38.0	39.0	35.0	39.0
10	37.53423350336545	39.0	37.0	39.0	35.0	39.0
11	37.38702495656587	39.0	37.0	39.0	34.0	39.0
12	36.988153317848926	39.0	35.0	39.0	33.0	39.0
13	36.84861881318323	39.0	35.0	39.0	33.0	39.0
14	38.11304802018159	40.0	36.0	41.0	33.0	41.0
15	38.22446610285647	40.0	36.0	41.0	33.0	41.0
16	38.25589500238934	40.0	36.0	41.0	34.0	41.0
17	38.16706711267684	40.0	36.0	41.0	34.0	41.0
18	38.01469314574234	39.0	36.0	41.0	34.0	41.0
19	37.85575053065579	39.0	36.0	41.0	34.0	41.0
20	37.588725277737645	39.0	35.0	41.0	34.0	41.0
21	37.53002307843333	39.0	35.0	41.0	33.0	41.0
22	37.45063919481087	39.0	35.0	41.0	33.0	41.0
23	37.401729215518486	39.0	35.0	41.0	33.0	41.0
24	37.32550741065905	39.0	35.0	41.0	33.0	41.0
25	37.31413489214629	39.0	35.0	41.0	33.0	41.0
26	37.27242701399153	39.0	35.0	41.0	33.0	41.0
27	37.20791556923715	39.0	35.0	41.0	33.0	41.0
28	37.12920736879929	39.0	35.0	41.0	33.0	41.0
29	37.06886041437457	39.0	35.0	41.0	33.0	41.0
30	36.90291944033873	39.0	35.0	41.0	32.0	41.0
31	36.66026249402665	39.0	35.0	41.0	31.0	41.0
32	36.3952991120545	39.0	35.0	41.0	31.0	41.0
33	36.0586733049576	39.0	35.0	41.0	30.0	41.0
34	35.67958614404943	39.0	35.0	41.0	26.0	41.0
35	35.4718113421424	39.0	35.0	41.0	24.0	41.0
36	35.299869975439805	39.0	35.0	41.0	24.0	41.0
37	35.26256366943386	39.0	35.0	41.0	23.0	41.0
38	35.22244349858677	39.0	35.0	41.0	23.0	41.0
39	35.14510148213181	39.0	35.0	41.0	23.0	41.0
40	35.01678317015436	38.0	35.0	41.0	23.0	41.0
41	34.93029942692879	38.0	35.0	41.0	22.0	41.0
42	34.879612815754086	38.0	35.0	41.0	22.0	41.0
43	34.74993795124264	38.0	35.0	41.0	21.0	41.0
44	34.60244935154418	38.0	34.0	41.0	20.0	41.0
45	34.53911664795943	38.0	34.0	40.0	20.0	41.0
46	34.46823955636064	38.0	34.0	40.0	20.0	41.0
47	34.444638061263426	38.0	34.0	40.0	20.0	41.0
48	34.378398882751924	38.0	34.0	40.0	20.0	41.0
49	34.249410073754674	38.0	34.0	40.0	20.0	41.0
50	34.13284953824611	38.0	34.0	40.0	18.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	3.0
11	4.0
12	7.0
13	5.0
14	11.0
15	32.0
16	71.0
17	161.0
18	352.0
19	807.0
20	1519.0
21	2636.0
22	3972.0
23	6468.0
24	10060.0
25	16455.0
26	25919.0
27	32057.0
28	31028.0
29	28173.0
30	28121.0
31	30555.0
32	36596.0
33	46327.0
34	79045.0
35	152586.0
36	78907.0
37	99492.0
38	170569.0
39	467805.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	26.53078914906149	26.488558950023894	28.145538601735883	18.835113299178737
2	33.19049153729038	27.26329788219256	28.211921511100247	11.334289069416815
3	27.7889527281079	24.698591215377718	37.03203197641036	10.48042408010402
4	26.921900062604422	26.05699595108706	36.12415678517053	10.896947201137992
5	21.12769449044079	33.037129235522265	34.47125197722533	11.36392429681162
6	19.52220604632727	41.291873650208	30.90820858754802	8.27771171591671
7	76.7295303927779	6.848182434459843	14.469622039718613	1.952665133043649
8	77.66615175459069	6.229843414867252	13.808386028472045	2.295618802070021
9	69.29271825418876	11.036380946030548	15.927601139474493	3.743299660306206
10	34.2375782092173	36.15327339608593	20.937436330566143	8.671712064130633
11	32.97926645403391	22.943074432578005	32.386932346480265	11.690726766907824
12	32.71610563476805	23.017532941407453	32.30491685466514	11.96144456915936
13	17.931831568185103	36.425324783570225	30.95755124116037	14.685292407084303
14	12.526588355578275	38.36124601313581	32.89754731449273	16.21461831679317
15	15.003945189646933	28.078785252029085	43.618905793316515	13.298363765007466
16	15.017355130043084	26.258737761577187	40.81059755731638	17.91330955106335
17	13.608940948105012	28.9622113806682	33.19071380149584	24.238133869730948
18	15.532859910575702	32.23905256178019	37.04403424350525	15.184053284138857
19	22.423050279867677	28.742762521809674	31.693764377715787	17.140422820606855
20	21.977929164397718	27.28441298171136	35.65303075766163	15.084627096229289
21	16.55223764488848	32.802492322623905	33.921296244846246	16.72397378764137
22	19.071306061515322	30.136581354255803	30.815376237733794	19.976736346495077
23	18.403624388310387	30.394407832590602	31.83534667659447	19.366621102504546
24	14.939044041652313	28.34883626166424	42.9482605973721	13.763859099311352
25	13.518553504550859	35.21509618483491	36.09533652652909	15.17101378408514
26	14.180085868071377	34.964678513348815	33.048835150343216	17.806400468236593
27	17.263260838158317	34.284994573048984	31.714731301097615	16.73701328769508
28	13.415793353559376	33.145816431992706	37.452259500868685	15.986130713579232
29	13.31629307758132	29.814965048953688	36.30811745922378	20.560624414241207
30	14.187272410714616	35.662439942359484	33.981826196800135	16.168461450125765
31	21.46516564239912	34.37026993987754	29.485791760665904	14.678772657057443
32	19.92346702525292	32.06094484513741	30.115243990531543	17.900344139078122
33	18.149205961125993	35.77898047408955	29.212406787948836	16.859406776835627
34	15.227987508751653	31.814602017418103	32.39797146868483	20.559439005145418
35	20.243157040774367	29.05148750319505	32.546443957932794	18.158911498097787
36	21.184520038970327	33.84943081841385	29.22070465161938	15.745344490996446
37	15.309188031813417	36.39650452492879	33.09091717324383	15.203390270013967
38	14.85502817198804	34.4662880766367	33.03327665596094	17.64540709541432
39	18.17669263453467	33.16004134114222	31.915361790560436	16.74790423376267
40	18.042296878299236	33.298067412733516	30.847826811731103	17.811808897236144
41	14.010127838962175	32.81234603573267	31.661610155992427	21.515915969312722
42	15.71904322668356	32.355148565099334	29.15572941555627	22.770078792660836
43	19.007368058411032	30.51413415126561	30.096129268861898	20.382368521461462
44	16.36234992535627	33.713405124671695	29.007182838239814	20.917062111732214
45	14.728041222601307	39.85678776361461	27.17261408636446	18.242556927419624
46	16.95246139085531	38.321016191947365	29.19944137596361	15.527081041233714
47	18.824518705385092	30.97977766170647	32.444350599557694	17.751353033350746
48	17.44040540991076	29.44971087131273	34.14437541906064	18.96550829971587
49	16.215433285546528	33.102252647722345	32.207120604262286	18.475193462468837
50	15.335933824537227	35.0669200478609	32.051609748508056	17.54553637909383
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	3266.0
1	5075.5
2	6885.0
3	19496.5
4	32108.0
5	23290.0
6	14472.0
7	14688.5
8	14905.0
9	15201.5
10	15498.0
11	15450.5
12	15403.0
13	14443.5
14	13484.0
15	12780.5
16	12077.0
17	10956.0
18	9835.0
19	9614.5
20	9394.0
21	9632.0
22	9870.0
23	11873.0
24	13876.0
25	20667.0
26	27458.0
27	37000.0
28	46542.0
29	56301.5
30	66061.0
31	73266.0
32	80471.0
33	84080.0
34	87689.0
35	89909.0
36	92129.0
37	87771.5
38	83414.0
39	88325.5
40	93237.0
41	98508.5
42	103780.0
43	100708.0
44	97636.0
45	93548.0
46	89460.0
47	84818.5
48	80177.0
49	71827.5
50	63478.0
51	52722.0
52	41966.0
53	35835.5
54	29705.0
55	25138.5
56	20572.0
57	18855.0
58	17138.0
59	16811.5
60	16485.0
61	15247.5
62	14010.0
63	12043.0
64	10076.0
65	7987.5
66	5899.0
67	5181.5
68	4464.0
69	3529.0
70	2594.0
71	2189.0
72	1784.0
73	1417.5
74	1051.0
75	849.5
76	648.0
77	525.5
78	403.0
79	260.0
80	117.0
81	117.5
82	118.0
83	81.5
84	45.0
85	47.0
86	49.0
87	29.5
88	10.0
89	7.0
90	4.0
91	2.0
92	0.0
93	0.0
94	0.0
95	1.0
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1349745.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	22.373094324782063
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.70161528026307	17.384255678532533
2	8.658355881804193	3.8742842568227362
3	3.376940410195288	2.2665781897940227
4	1.7369542773809605	1.5544416754271162
5	1.0966825975549408	1.2268091599721849
6	0.7324646611315971	0.9832500571840054
7	0.5876897796203817	0.920390721121003
8	0.46758097376992885	0.8368986584502454
9	0.3900270712181854	0.7853501208224658
>10	4.03065942882043	20.285598789961362
>50	0.6286621472109638	9.766912690485185
>100	0.5184464213230471	24.90799402257333
>500	0.06359875817129008	9.012327243494813
>1k	0.008657422578290797	2.6262580889846876
>5k	0.0013319111658908918	2.1627350461326547
>10k+	3.3297779147272295E-4	1.405915600241654
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	18872	1.3981900284868625	No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA	8824	0.6537531163293807	No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA	8264	0.6122637979766549	No Hit
GAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA	6287	0.4657916865778351	No Hit
AAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA	5656	0.41904211536253144	No Hit
GAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA	2167	0.16054884441135178	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2138	0.15840029042522846	No Hit
AGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	1886	0.1397300971665018	No Hit
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGA	1870	0.13854468807070966	No Hit
GGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGA	1709	0.12661650904430094	No Hit
GAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA	1580	0.11705914820947662	No Hit
TAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA	1578	0.11691097207250259	No Hit
AAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA	1520	0.11261386410025596	No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1484	0.10994669363472359	No Hit
AAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA	1375	0.10187109416963944	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.09075788389658787	0.0
2	0.0	0.0	0.0	0.3496956832586896	0.0
3	0.0	0.0	0.0	0.5365457919829301	0.0
4	0.0	0.0	0.0	0.761180815635546	0.0
5	0.0	0.0	0.0	1.325583721369592	0.0
6	0.0	0.0	0.0	1.7519605555123374	0.0
7	0.0	0.0	0.0	2.0398667896528604	0.0
8	0.0	0.0	0.0	2.7320716135269993	0.0
9	0.0	0.0	0.0	2.994491552107991	0.0
10	0.0	0.0	0.0	3.490362994491552	0.0
11	0.0	0.0	0.0	4.063063763896143	0.0
12	0.0	0.0	0.0	4.561157848334315	0.0
13	0.0	0.0	0.0	4.820169735764904	0.0
14	0.0	0.0	0.0	4.893739187772505	0.0
15	0.0	0.0	0.0	5.019837080337397	0.0
16	0.0	0.0	0.0	5.275663180823044	0.0
17	0.0	7.408806848701051E-5	0.0	5.584462250276904	0.0
18	0.0	7.408806848701051E-5	0.0	6.014469399775513	0.0
19	0.0	7.408806848701051E-5	0.0	6.228139389292052	0.0
20	0.0	7.408806848701051E-5	0.0	6.457219697053888	0.0
21	0.0	7.408806848701051E-5	0.0	6.764685181274982	0.0
22	0.0	7.408806848701051E-5	0.0	7.098155577535016	0.0
23	0.0	7.408806848701051E-5	0.0	7.463409755175977	0.0
24	0.0	7.408806848701051E-5	0.0	7.73894328187917	0.0
25	0.0	7.408806848701051E-5	0.0	7.9650600669015255	0.0
26	0.0	7.408806848701051E-5	0.0	8.188657857595324	0.0
27	0.0	7.408806848701051E-5	0.0	8.400179293125738	0.0
28	0.0	7.408806848701051E-5	0.0	8.63037092191488	0.0
29	0.0	7.408806848701051E-5	0.0	8.868860414374566	0.0
30	0.0	7.408806848701051E-5	0.0	9.187290932731738	0.0
31	0.0	7.408806848701051E-5	0.0	9.500461198226331	0.0
32	0.0	7.408806848701051E-5	0.0	9.771179000477868	0.0
33	0.0	7.408806848701051E-5	0.0	10.025819691867722	0.0
34	0.0	7.408806848701051E-5	0.0	10.293499883311291	0.0
35	0.0	7.408806848701051E-5	0.0	10.674905259882422	0.0
36	0.0	7.408806848701051E-5	0.0	10.952957780914172	0.0
37	0.0	7.408806848701051E-5	0.0	11.236566907082448	0.0
38	0.0	7.408806848701051E-5	0.0	11.498023700773109	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTTAGCG	65	0.0	44.000004	1
ACGCGAG	40	8.314601E-9	44.0	1
TAATACG	55	1.8189894E-12	44.0	1
GGTACGC	20	7.85697E-4	44.0	16
ACCGGTT	25	4.4432552E-5	44.0	31
GTCGATA	25	4.4432552E-5	44.0	30
GTCGAAT	25	4.4432552E-5	44.0	12
ATGTGCG	55	1.8189894E-12	44.0	1
ACCGACT	25	4.4432552E-5	44.0	25
TAACGTG	35	1.446333E-7	44.0	27
CGCGAGG	60	0.0	44.0	2
ATCCGTT	85	0.0	44.0	22
ATCGTAG	35	1.446333E-7	44.0	1
TGTTGCG	20	7.85697E-4	44.0	1
ACGATGT	20	7.85697E-4	44.0	9
ATATCGT	25	4.4432552E-5	44.0	29
CGAGGGT	60	0.0	44.0	4
ACTACGG	80	0.0	44.0	2
TATTACG	60	0.0	44.0	1
GCGATCG	25	4.4432552E-5	44.0	9
>>END_MODULE