FastQCFastQC Report
Thu 26 May 2016
SRR1546162_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546162_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1383764
Sequences flagged as poor quality0
Sequence length50
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT193001.3947465030164103No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA93750.677499920506676No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA82610.5969948632859361No Hit
GAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA62450.4513052803801805No Hit
AAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA58640.4237716836107891No Hit
GAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA22340.16144371439060418No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA21250.15356664864817987No Hit
AGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG19080.1378847838215187No Hit
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGA18740.13542771744314783No Hit
GGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGA18220.13166985121740413No Hit
AAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA16020.1157711864161808No Hit
TAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA15990.11555438644161867No Hit
GAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA15840.11447038656880798No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15300.11056798702668953No Hit
AAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA14400.10406398778982544No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGTTG650.044.0000041
TCACGAG207.8570395E-444.01
CGAACTA254.4433124E-544.027
AGATCGC207.8570395E-444.019
ACGTTAG207.8570395E-444.01
TAGGTCG207.8570395E-444.03
GACCGTA254.4433124E-544.024
AAGACCG254.4433124E-544.022
CCTACGG254.4433124E-544.01
ATAGACG502.7284841E-1144.01
TAACGTT207.8570395E-444.020
TCGATAG207.8570395E-444.01
CGTTAAT207.8570395E-444.018
TAGCACG254.4433124E-544.01
TACGCGG207.8570395E-444.02
TACGCCA254.4433124E-544.024
TGACATA207.8570395E-444.043
TGATTCG502.7284841E-1144.015
ACGGATG207.8570395E-444.05
AGACCAC502.7284841E-1144.040
Produced by FastQC (version 0.11.5)