##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546162_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1383764
Sequences flagged as poor quality	0
Sequence length	50
%GC	37
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.827459740244723	31.0	31.0	34.0	30.0	34.0
2	32.235750460338615	33.0	31.0	34.0	30.0	34.0
3	32.283486924070864	34.0	31.0	34.0	30.0	34.0
4	35.958909178154656	37.0	35.0	37.0	35.0	37.0
5	35.850523644205225	37.0	35.0	37.0	35.0	37.0
6	35.94507878511076	37.0	35.0	37.0	35.0	37.0
7	36.13228700847832	37.0	35.0	37.0	35.0	37.0
8	36.01267268117974	37.0	35.0	37.0	35.0	37.0
9	37.672856065051555	39.0	38.0	39.0	35.0	39.0
10	37.52327853593532	39.0	37.0	39.0	35.0	39.0
11	37.37981476610173	39.0	37.0	39.0	34.0	39.0
12	36.98052124495217	39.0	35.0	39.0	33.0	39.0
13	36.810649070217174	39.0	35.0	39.0	33.0	39.0
14	38.06716101878644	40.0	36.0	41.0	33.0	41.0
15	38.20413596538138	40.0	36.0	41.0	33.0	41.0
16	38.22049280079551	40.0	36.0	41.0	34.0	41.0
17	38.13911982100994	40.0	36.0	41.0	34.0	41.0
18	37.999561341384805	39.0	36.0	41.0	34.0	41.0
19	37.8413508372815	39.0	36.0	41.0	34.0	41.0
20	37.57477720189281	39.0	35.0	41.0	34.0	41.0
21	37.51551998751232	39.0	35.0	41.0	33.0	41.0
22	37.43760713532076	39.0	35.0	41.0	33.0	41.0
23	37.38004891007426	39.0	35.0	41.0	33.0	41.0
24	37.305652553470104	39.0	35.0	41.0	33.0	41.0
25	37.28692103566793	39.0	35.0	41.0	33.0	41.0
26	37.256902188523476	39.0	35.0	41.0	33.0	41.0
27	37.18175859467366	39.0	35.0	41.0	33.0	41.0
28	37.10229200933107	39.0	35.0	41.0	33.0	41.0
29	37.03512665454514	39.0	35.0	41.0	33.0	41.0
30	36.87736853972209	39.0	35.0	41.0	32.0	41.0
31	36.649116467837004	39.0	35.0	41.0	31.0	41.0
32	36.3928531165719	39.0	35.0	41.0	31.0	41.0
33	36.03780124356465	39.0	35.0	41.0	30.0	41.0
34	35.721222694043206	39.0	35.0	41.0	27.0	41.0
35	35.48310116464946	39.0	35.0	41.0	25.0	41.0
36	35.30332628974305	39.0	35.0	41.0	24.0	41.0
37	35.21013482067752	39.0	35.0	41.0	23.0	41.0
38	35.18721328203364	39.0	35.0	41.0	23.0	41.0
39	35.11347888801848	39.0	35.0	41.0	23.0	41.0
40	35.00976972952035	38.0	35.0	41.0	23.0	41.0
41	34.94280744404393	38.0	35.0	41.0	22.0	41.0
42	34.86259217612252	38.0	35.0	41.0	22.0	41.0
43	34.763947465030164	38.0	35.0	41.0	21.0	41.0
44	34.60749593138714	38.0	34.0	41.0	20.0	41.0
45	34.53852246481336	38.0	34.0	40.0	20.0	41.0
46	34.48623536961505	38.0	34.0	40.0	20.0	41.0
47	34.439238916462635	38.0	34.0	40.0	20.0	41.0
48	34.387285693225145	38.0	34.0	40.0	20.0	41.0
49	34.25387638354517	38.0	34.0	40.0	20.0	41.0
50	34.11439306124455	38.0	34.0	40.0	18.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	4.0
10	5.0
11	5.0
12	6.0
13	8.0
14	16.0
15	27.0
16	62.0
17	186.0
18	402.0
19	864.0
20	1578.0
21	2582.0
22	4248.0
23	6647.0
24	10190.0
25	16575.0
26	26133.0
27	32930.0
28	32345.0
29	29442.0
30	29139.0
31	32087.0
32	37797.0
33	48022.0
34	82660.0
35	153371.0
36	81577.0
37	102344.0
38	175866.0
39	476645.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	26.613497677349606	26.60757181137824	28.142949231227288	18.635981280044863
2	33.323745956680476	27.196400542289002	28.17575829404436	11.304095206986164
3	27.707759415622895	24.74778936292605	36.97913806111447	10.56531316033659
4	26.954813103968593	26.130611867341543	36.04581417062447	10.868760858065393
5	21.171601515865422	32.968410798373135	34.42429489421607	11.43569279154538
6	19.61396596529466	41.19040530032578	30.89363504181349	8.301993692566073
7	76.67181687050683	6.820310399750247	14.519961496324518	1.9879112334184152
8	77.55621623340396	6.29529312801894	13.813988512492015	2.334502126085084
9	69.1762468166537	11.090041365435146	15.99188879028505	3.7418230276260984
10	34.261478113319896	35.974486978993525	20.9982338028739	8.76580110481267
11	32.948392934055235	22.936281042143026	32.38377353363724	11.731552490164509
12	32.601512974755806	23.082042891706966	32.309917008969734	12.006527124567484
13	18.020702952237517	36.35179120138983	30.957663300967507	14.66984254540514
14	12.590947589328817	38.277697642083474	32.923244137006016	16.208110631581686
15	15.021997970752238	28.06815324000335	43.60136555077311	13.3084832384713
16	15.079883563960328	26.32500917786559	40.71604695598383	17.879060302190258
17	13.777060250158263	28.96621100129791	33.14409104442665	24.112637704117176
18	15.57144137295088	32.143197828531456	37.003491924923615	15.281868873594052
19	22.470594696783554	28.68935743378206	31.714078412214803	17.125969457219583
20	22.010400617446326	27.242940270161675	35.630136352730666	15.11652275966133
21	16.58620978721805	32.85083294550227	33.84529442881879	16.717662838460896
22	19.03062950040614	30.101303401447066	30.853816113152245	20.01425098499455
23	18.51862022714856	30.35517617165933	31.77911840458344	19.34708519660867
24	14.963534244278648	28.36885480472104	42.793062978947276	13.874547972053039
25	13.622915468244583	35.123836145469895	36.09676216464657	15.156486221638952
26	14.201410067034553	34.84987324428154	33.21180490314822	17.736911785535682
27	17.32152303427463	34.08160640109151	31.794366669460977	16.802503895172876
28	13.481561884830073	33.1432238445284	37.441427873539126	15.933786397102395
29	13.390794962146725	29.73512824441162	36.32808773750437	20.545989055937284
30	14.30981005431562	35.499550501386075	33.93215895196002	16.25848049233829
31	21.40263802209047	34.33945383750408	29.518545069824043	14.7393630705814
32	19.91177686368485	32.12093969780974	30.101881534712565	17.865401903792844
33	18.198623464694847	35.61228648815838	29.279559231198384	16.909530815948383
34	15.322844068786296	31.815107200360753	32.24155275032447	20.62049598052847
35	20.21175576182066	29.07641765503366	32.46709699052729	18.244729592618395
36	21.139948719579348	33.87391202546099	29.160102445214648	15.826036809745014
37	15.37509286265577	36.27424907715478	33.05397452166699	15.296683538522466
38	14.922342249111843	34.46368022292819	32.8952769402875	17.718700587672465
39	18.16552533524503	33.091914517215365	31.91519652195027	16.827363625589335
40	18.045129082704854	33.23926623325943	30.87954304346695	17.83606164056877
41	14.105945811569025	32.63316577104188	31.711404545861864	21.549483871527226
42	15.821122676988272	32.27985407916379	29.161186445087456	22.737836798760483
43	19.05454976426616	30.396295900167946	30.11423913326261	20.434915202303284
44	16.358569813927808	33.70408537872065	29.008559262995714	20.92878554435583
45	14.700122275185654	39.74377133673083	27.180212810855032	18.375893577228485
46	16.960912409919608	38.20550325055429	29.16819631093163	15.66538802859447
47	18.741707400973	30.882505976452634	32.503808452886474	17.871978169687893
48	17.45861288485609	29.396342150829184	34.076475468360215	19.068569495954513
49	16.137289306558056	33.06416412047141	32.20737062100184	18.59117595196869
50	15.420404057339256	35.015147091556074	31.887590658522697	17.676858192581975
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	3255.0
1	5212.5
2	7170.0
3	20058.0
4	32946.0
5	23718.5
6	14491.0
7	14861.5
8	15232.0
9	15524.0
10	15816.0
11	15796.0
12	15776.0
13	14806.0
14	13836.0
15	13042.0
16	12248.0
17	11209.5
18	10171.0
19	9815.5
20	9460.0
21	9799.0
22	10138.0
23	12178.0
24	14218.0
25	21255.5
26	28293.0
27	38117.0
28	47941.0
29	57498.5
30	67056.0
31	74347.0
32	81638.0
33	85076.5
34	88515.0
35	91290.5
36	94066.0
37	89685.0
38	85304.0
39	90249.5
40	95195.0
41	100690.5
42	106186.0
43	103102.0
44	100018.0
45	96412.0
46	92806.0
47	87636.5
48	82467.0
49	74646.5
50	66826.0
51	55453.0
52	44080.0
53	37228.0
54	30376.0
55	25746.0
56	21116.0
57	19377.5
58	17639.0
59	17396.0
60	17153.0
61	15773.5
62	14394.0
63	12422.5
64	10451.0
65	8291.5
66	6132.0
67	5243.5
68	4355.0
69	3531.0
70	2707.0
71	2272.5
72	1838.0
73	1477.0
74	1116.0
75	861.0
76	606.0
77	505.0
78	404.0
79	259.5
80	115.0
81	105.0
82	95.0
83	82.5
84	70.0
85	53.5
86	37.0
87	21.0
88	5.0
89	4.0
90	3.0
91	2.5
92	2.0
93	1.5
94	1.0
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1383764.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	22.455830120830463
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.55051508351998	17.41461192498425
2	8.963575690296265	4.025690659529971
3	3.242994508606224	2.184724013041423
4	1.6944105757256314	1.5219758417373326
5	1.1213159605653642	1.2590040361115824
6	0.7951829846532434	1.07138964110089
7	0.5776629809059125	0.9080331236420989
8	0.460801514565135	0.8278144424396845
9	0.4012087545792765	0.8108528072239973
>10	3.983626480757755	20.098169924888424
>50	0.6208881985003931	9.674315943618335
>100	0.5135700780157791	24.76744171840906
>500	0.06387203621786508	9.151777906814202
>1k	0.008754035420722625	2.713374482145058
>5k	0.001296894136403352	2.165646773945604
>10k+	3.24223534100838E-4	1.405176760368134
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	19300	1.3947465030164103	No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA	9375	0.677499920506676	No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA	8261	0.5969948632859361	No Hit
GAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA	6245	0.4513052803801805	No Hit
AAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA	5864	0.4237716836107891	No Hit
GAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA	2234	0.16144371439060418	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2125	0.15356664864817987	No Hit
AGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	1908	0.1378847838215187	No Hit
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGA	1874	0.13542771744314783	No Hit
GGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGA	1822	0.13166985121740413	No Hit
AAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA	1602	0.1157711864161808	No Hit
TAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA	1599	0.11555438644161867	No Hit
GAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA	1584	0.11447038656880798	No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1530	0.11056798702668953	No Hit
AAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA	1440	0.10406398778982544	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.09344078903628075	0.0
2	0.0	0.0	0.0	0.35179409205615986	0.0
3	0.0	0.0	0.0	0.5425058030126525	0.0
4	0.0	0.0	0.0	0.7775169754380082	0.0
5	0.0	0.0	0.0	1.3420641091978112	0.0
6	0.0	0.0	0.0	1.7857091238101295	0.0
7	0.0	0.0	0.0	2.0877837550333727	0.0
8	0.0	0.0	0.0	2.7973700717752448	0.0
9	0.0	0.0	0.0	3.057819107882558	0.0
10	0.0	0.0	0.0	3.5646974484088325	0.0
11	0.0	0.0	0.0	4.139361914314868	0.0
12	0.0	0.0	0.0	4.661994386325992	0.0
13	0.0	0.0	0.0	4.918107422942063	0.0
14	0.0	0.0	0.0	4.990229547813066	0.0
15	0.0	0.0	0.0	5.127174865078149	0.0
16	0.0	0.0	0.0	5.4012100329246895	0.0
17	0.0	0.0	0.0	5.736527326914127	0.0
18	0.0	0.0	0.0	6.18472514099225	0.0
19	0.0	0.0	0.0	6.406294714994753	0.0
20	0.0	0.0	0.0	6.639571487623612	0.0
21	0.0	0.0	0.0	6.96137491653201	0.0
22	0.0	0.0	0.0	7.307315409274992	0.0
23	0.0	0.0	0.0	7.689389231111663	0.0
24	0.0	0.0	0.0	7.970651064776941	0.0
25	0.0	0.0	0.0	8.203205170823926	0.0
26	0.0	0.0	0.0	8.445587542384395	0.0
27	0.0	0.0	0.0	8.67149311587814	0.0
28	0.0	0.0	0.0	8.909611754605555	0.0
29	0.0	0.0	0.0	9.162689591577754	0.0
30	0.0	0.0	0.0	9.490852486406641	0.0
31	0.0	0.0	0.0	9.82862684677445	0.0
32	0.0	0.0	0.0	10.111406280261663	0.0
33	0.0	0.0	0.0	10.375685449252908	0.0
34	0.0	0.0	0.0	10.663668082129611	0.0
35	0.0	0.0	0.0	11.060845635527446	0.0
36	0.0	0.0	0.0	11.357428000728447	0.0
37	0.0	0.0	0.0	11.654516232536762	0.0
38	0.0	0.0	0.0	11.930863933445298	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACCGTTG	65	0.0	44.000004	1
TCACGAG	20	7.8570395E-4	44.0	1
CGAACTA	25	4.4433124E-5	44.0	27
AGATCGC	20	7.8570395E-4	44.0	19
ACGTTAG	20	7.8570395E-4	44.0	1
TAGGTCG	20	7.8570395E-4	44.0	3
GACCGTA	25	4.4433124E-5	44.0	24
AAGACCG	25	4.4433124E-5	44.0	22
CCTACGG	25	4.4433124E-5	44.0	1
ATAGACG	50	2.7284841E-11	44.0	1
TAACGTT	20	7.8570395E-4	44.0	20
TCGATAG	20	7.8570395E-4	44.0	1
CGTTAAT	20	7.8570395E-4	44.0	18
TAGCACG	25	4.4433124E-5	44.0	1
TACGCGG	20	7.8570395E-4	44.0	2
TACGCCA	25	4.4433124E-5	44.0	24
TGACATA	20	7.8570395E-4	44.0	43
TGATTCG	50	2.7284841E-11	44.0	15
ACGGATG	20	7.8570395E-4	44.0	5
AGACCAC	50	2.7284841E-11	44.0	40
>>END_MODULE