FastQCFastQC Report
Thu 26 May 2016
SRR1546161_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546161_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences169301
Sequences flagged as poor quality0
Sequence length50
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28351.6745323418054232No Hit
GGCTCTGGGCTCCTCTACAAAAACAAAAGTTAAAAAAAAACACACAAAAA2440.14412200754868548No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCACGA207.837451E-444.00000424
GTACAGG207.837451E-444.0000042
TTCAACC207.837451E-444.00000425
CGCGAGG207.837451E-444.0000042
TATTGCG207.837451E-444.0000041
GCCGATT207.837451E-444.0000049
TGCGAAG207.837451E-444.0000041
TAGGGTC207.837451E-444.0000045
TTGCGAG207.837451E-444.0000041
CGGTAGT207.837451E-444.00000412
CGCCGGT207.837451E-444.00000428
GTTATAC207.837451E-444.00000431
TAGACGG302.5148984E-644.02
GTGGGAT454.7475623E-1044.05
TGAGTAG254.4267224E-544.01
CGAGGGT254.4267224E-544.04
CGGGATC351.4363286E-744.06
CGGGATA351.4363286E-744.06
TGGGCGA700.044.06
CGTTTTT17250.042.4695661
Produced by FastQC (version 0.11.5)