##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546161_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	169301
Sequences flagged as poor quality	0
Sequence length	50
%GC	39
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.88258781696505	31.0	31.0	34.0	30.0	34.0
2	32.262000815116274	33.0	31.0	34.0	31.0	34.0
3	32.21019367871424	33.0	31.0	34.0	30.0	34.0
4	35.950691372171455	37.0	35.0	37.0	35.0	37.0
5	35.95464882073939	37.0	35.0	37.0	35.0	37.0
6	35.99155941193496	37.0	35.0	37.0	35.0	37.0
7	36.15555135527847	37.0	35.0	37.0	35.0	37.0
8	36.04098617255657	37.0	35.0	37.0	35.0	37.0
9	37.73087577746144	39.0	38.0	39.0	35.0	39.0
10	37.47020986290689	39.0	37.0	39.0	35.0	39.0
11	37.37641833184683	39.0	37.0	39.0	34.0	39.0
12	37.19587598419383	39.0	37.0	39.0	34.0	39.0
13	37.1432537315196	39.0	37.0	39.0	33.0	39.0
14	38.389542885157205	40.0	38.0	41.0	34.0	41.0
15	38.47788849445662	40.0	38.0	41.0	34.0	41.0
16	38.50085350942995	40.0	38.0	41.0	34.0	41.0
17	38.40478792210324	40.0	37.0	41.0	34.0	41.0
18	38.20109154700799	40.0	37.0	41.0	34.0	41.0
19	38.037129136862745	40.0	37.0	41.0	34.0	41.0
20	37.80127701549311	40.0	35.0	41.0	34.0	41.0
21	37.73694189638573	40.0	35.0	41.0	33.0	41.0
22	37.629801359708445	39.0	35.0	41.0	33.0	41.0
23	37.572282502761354	39.0	35.0	41.0	33.0	41.0
24	37.49144423246171	39.0	35.0	41.0	33.0	41.0
25	37.473110022976826	39.0	35.0	41.0	33.0	41.0
26	37.432265609771946	39.0	35.0	41.0	33.0	41.0
27	37.37201197866522	39.0	35.0	41.0	33.0	41.0
28	37.28183531107318	39.0	35.0	41.0	33.0	41.0
29	37.20058948263743	39.0	35.0	41.0	33.0	41.0
30	37.01698749564385	39.0	35.0	41.0	32.0	41.0
31	36.80414764236478	39.0	35.0	41.0	31.0	41.0
32	36.544922947885716	39.0	35.0	41.0	31.0	41.0
33	36.19780745536057	39.0	35.0	41.0	30.0	41.0
34	35.76148398414658	39.0	35.0	41.0	26.0	41.0
35	35.53687810467747	39.0	35.0	41.0	24.0	41.0
36	35.3766900372709	39.0	35.0	41.0	23.0	41.0
37	35.31119721679139	39.0	35.0	41.0	23.0	41.0
38	35.22312331291605	39.0	35.0	41.0	23.0	41.0
39	35.12102113986332	39.0	35.0	41.0	22.0	41.0
40	35.00807437640652	39.0	35.0	41.0	22.0	41.0
41	34.88261735016332	39.0	35.0	41.0	21.0	41.0
42	34.82289531662542	39.0	35.0	41.0	20.0	41.0
43	34.71570752683091	38.0	34.0	41.0	20.0	41.0
44	34.60150855576754	38.0	34.0	41.0	18.0	41.0
45	34.54185149526583	38.0	34.0	40.0	20.0	41.0
46	34.47431497746617	38.0	34.0	40.0	20.0	41.0
47	34.41345296247512	38.0	34.0	40.0	20.0	41.0
48	34.312366731442815	38.0	34.0	40.0	19.0	41.0
49	34.2304416394469	38.0	34.0	40.0	19.0	41.0
50	34.116413960933485	38.0	34.0	40.0	18.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	4.0
15	1.0
16	9.0
17	25.0
18	63.0
19	105.0
20	197.0
21	327.0
22	547.0
23	759.0
24	1288.0
25	2063.0
26	3218.0
27	4105.0
28	3981.0
29	3573.0
30	3378.0
31	3612.0
32	4364.0
33	5596.0
34	9069.0
35	14417.0
36	10989.0
37	14427.0
38	25186.0
39	57998.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.80311988706505	20.90418839817839	27.288675199792085	20.00401651496447
2	37.27680285408828	23.452903408721745	27.831495383961112	11.438798353228865
3	27.36782417115079	23.952605123419236	36.973201575891466	11.706369129538514
4	24.16996946267299	25.87580699464268	36.43865068723752	13.515572855446809
5	21.941984985321998	28.500717656717917	36.13091476128316	13.426382596676925
6	20.33242567970656	36.82967023230814	34.062409554580306	8.775494533405
7	77.22399749558478	4.750119609452986	15.746510652624615	2.2793722423376117
8	77.8300187240477	4.588277682943397	15.25803155326903	2.3236720397398716
9	70.78576027312302	7.379164919285769	17.95264056325716	3.8824342443340556
10	32.540268515838655	29.86337942481143	25.4227677332089	12.173584326141015
11	26.939002132296913	24.70570167925765	34.68201605424658	13.673280134198853
12	25.18591148309815	23.209549854991995	36.36127370777491	15.243264954134943
13	19.362555448579748	26.961447362980728	37.198835210660306	16.47716197777922
14	15.777225178823514	29.757059911046007	37.65837177571308	16.807343134417398
15	15.535052953024495	26.135108475437242	42.401403417581705	15.928435153956563
16	18.143425024069558	24.833875759741524	39.80366329791318	17.219035918275733
17	17.994577704797962	25.34598141771165	36.45400795033697	20.205432927153414
18	18.57874436654243	25.7913420475957	39.01689889604905	16.613014689812818
19	21.076071612099163	26.36546742192899	35.73044459276673	16.82801637320512
20	22.04239785943379	25.69565448520682	36.644792411149375	15.617155244210016
21	20.58345786498603	26.595235704455376	36.64006709942647	16.181239331132126
22	19.839811932593427	24.68148445667775	36.228964979533494	19.249738631195328
23	17.785482661059294	27.41153330458769	35.99801536907638	18.804968665276636
24	17.159969521739388	25.30227228427475	40.82669328592271	16.711064908063154
25	16.84632695613139	28.895281185580714	37.88577740237801	16.37261445590989
26	16.933745223005182	29.219555702565252	35.96730084287748	17.879398231552088
27	16.36316383246407	28.87460794679299	36.52784094600741	18.234387274735532
28	15.756551940035795	27.45287978216313	39.52605123419236	17.26451704360872
29	17.243253140855636	25.44462229992735	38.43745754602749	18.874667013189526
30	17.410411043053497	28.570416004630804	37.362449129065986	16.656723823249713
31	19.65965942315757	29.084884318462382	34.69914530924212	16.556310949137927
32	20.44524249709098	28.017554533050603	34.098440056467474	17.438762913390942
33	20.16585844147406	28.674963526500143	33.31344764649943	17.84573038552637
34	17.495466654065837	28.262089414711077	35.11379141292727	19.128652518295816
35	19.423393837012185	29.045900496748395	33.7924761224092	17.73822954383022
36	19.979208628419208	30.95906108056066	32.206543375408295	16.85518691561184
37	18.53976054482844	31.62592069745601	33.03347292691715	16.8008458307984
38	18.16291693492655	30.815529736977336	33.357156779936325	17.664396548159786
39	18.422809079686473	31.631827337109648	31.695028381403535	18.250335201800343
40	19.647846143850302	29.858063449123158	32.47293282378722	18.02115758323932
41	18.27337109644952	29.143950714998727	32.1208971004306	20.461781088121157
42	17.98867106514433	29.783639789487363	31.206549282047952	21.02113986332036
43	18.558071127754708	29.279212763066965	32.024028210110984	20.138687899067342
44	17.67857248332851	30.06243318113892	32.66194529270353	19.597049042829042
45	17.708105681596685	32.8013419885293	29.830302242751078	19.660250087122936
46	18.148150335792465	32.22367263040384	31.579848908157658	18.048328125646037
47	17.99930301652087	30.095510363199274	33.30990366270725	18.595282957572607
48	17.866994288279457	29.52374764472744	33.69796988795105	18.91128817904206
49	18.335981476778045	29.676729611756574	33.24020531479436	18.74708359667102
50	17.39918842771159	30.847425591106965	33.181138918258014	18.572247062923434
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	249.0
1	469.0
2	689.0
3	2823.5
4	4958.0
5	3448.5
6	1939.0
7	1953.0
8	1967.0
9	1987.0
10	2007.0
11	1974.0
12	1941.0
13	1894.0
14	1847.0
15	1763.0
16	1679.0
17	1587.0
18	1495.0
19	1541.5
20	1588.0
21	1737.0
22	1886.0
23	2004.0
24	2122.0
25	2497.5
26	2873.0
27	3770.0
28	4667.0
29	5245.0
30	5823.0
31	6837.0
32	7851.0
33	8408.5
34	8966.0
35	9900.5
36	10835.0
37	10729.0
38	10623.0
39	10647.5
40	10672.0
41	10436.5
42	10201.0
43	10070.5
44	9940.0
45	9490.5
46	9041.0
47	8832.5
48	8624.0
49	8383.0
50	8142.0
51	7682.5
52	7223.0
53	6469.0
54	5715.0
55	5210.0
56	4705.0
57	4487.5
58	4270.0
59	3996.5
60	3723.0
61	3402.5
62	3082.0
63	2760.5
64	2439.0
65	2082.0
66	1725.0
67	1503.5
68	1282.0
69	1116.5
70	951.0
71	813.0
72	675.0
73	551.0
74	427.0
75	320.5
76	214.0
77	160.0
78	106.0
79	92.5
80	79.0
81	58.0
82	37.0
83	24.5
84	12.0
85	7.5
86	3.0
87	3.0
88	3.0
89	3.0
90	3.0
91	2.0
92	1.0
93	1.0
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	169301.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	44.23659635796599
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.08799220220848	31.004542205893642
2	11.310803412869026	10.007028901187825
3	5.93780460123109	7.880047961913987
4	3.490312846327427	6.175982421840391
5	2.0656136087484813	4.568785772086403
6	1.5101544870682173	4.008245668956474
7	1.0521677593366536	3.2581024329448733
8	0.6836419959141709	2.4193596021287527
9	0.6168800822506777	2.4559807679812877
>10	3.183208043475358	24.69152574408893
>50	0.054744769204064465	1.501467799953928
>100	0.00534095309307946	0.35439837921807904
>500	0.0	0.0
>1k	0.001335238273269865	1.6745323418054232
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2835	1.6745323418054232	No Hit
GGCTCTGGGCTCCTCTACAAAAACAAAAGTTAAAAAAAAACACACAAAAA	244	0.14412200754868548	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0354398379218079	0.0
2	0.0	0.0	0.0	0.13467138410287002	0.0
3	0.0	0.0	0.0	0.20791371580793971	0.0
4	0.0	0.0	0.0	0.30950791785045567	0.0
5	0.0	0.0	0.0	0.5788506860561957	0.0
6	0.0	0.0	0.0	0.8605973975345687	0.0
7	0.0	0.0	0.0	1.0791430647190507	0.0
8	0.0	0.0	0.0	1.4849292089237511	0.0
9	0.0	0.0	0.0	1.6786669895629678	0.0
10	0.0	0.0	0.0	1.9999881867206928	0.0
11	0.0	0.0	0.0	2.285278881991246	0.0
12	0.0	0.0	0.0	2.5587562979545306	0.0
13	0.0	0.0	0.0	2.70524096136467	0.0
14	0.0	0.0	0.0	2.754266070489838	0.0
15	0.0	0.0	0.0	2.829280394090998	0.0
16	0.0	0.0	0.0	2.960407794401687	0.0
17	0.0	0.0	0.0	3.0879912109201952	0.0
18	0.0	0.0	0.0	3.266371728459962	0.0
19	0.0	0.0	0.0	3.383323193601928	0.0
20	0.0	0.0	0.0	3.4961400109863496	0.0
21	0.0	0.0	0.0	3.6337647149160373	0.0
22	0.0	0.0	0.0	3.819233200040165	0.0
23	0.0	0.0	0.0	3.979303134653664	0.0
24	0.0	0.0	0.0	4.108658543068263	0.0
25	0.0	0.0	0.0	4.228563328037047	0.0
26	0.0	0.0	0.0	4.3384268255946505	0.0
27	0.0	0.0	0.0	4.458331610563435	0.0
28	0.0	0.0	0.0	4.580008387428308	0.0
29	0.0	0.0	0.0	4.710545123773634	0.0
30	0.0	0.0	0.0	4.875931034075404	0.0
31	0.0	0.0	0.0	5.039544952481084	0.0
32	0.0	0.0	0.0	5.174807000549317	0.0
33	0.0	0.0	0.0	5.3236543198209105	0.0
34	0.0	0.0	0.0	5.4683669913349595	0.0
35	0.0	0.0	0.0	5.676871371108263	0.0
36	0.0	0.0	0.0	5.838713297617852	0.0
37	0.0	0.0	0.0	5.99346725654308	0.0
38	0.0	0.0	0.0	6.160034494775577	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTCACGA	20	7.837451E-4	44.000004	24
GTACAGG	20	7.837451E-4	44.000004	2
TTCAACC	20	7.837451E-4	44.000004	25
CGCGAGG	20	7.837451E-4	44.000004	2
TATTGCG	20	7.837451E-4	44.000004	1
GCCGATT	20	7.837451E-4	44.000004	9
TGCGAAG	20	7.837451E-4	44.000004	1
TAGGGTC	20	7.837451E-4	44.000004	5
TTGCGAG	20	7.837451E-4	44.000004	1
CGGTAGT	20	7.837451E-4	44.000004	12
CGCCGGT	20	7.837451E-4	44.000004	28
GTTATAC	20	7.837451E-4	44.000004	31
TAGACGG	30	2.5148984E-6	44.0	2
GTGGGAT	45	4.7475623E-10	44.0	5
TGAGTAG	25	4.4267224E-5	44.0	1
CGAGGGT	25	4.4267224E-5	44.0	4
CGGGATC	35	1.4363286E-7	44.0	6
CGGGATA	35	1.4363286E-7	44.0	6
TGGGCGA	70	0.0	44.0	6
CGTTTTT	1725	0.0	42.469566	1
>>END_MODULE