FastQCFastQC Report
Thu 26 May 2016
SRR1546160_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546160_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences172051
Sequences flagged as poor quality0
Sequence length50
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28631.6640414760739546No Hit
GGCTCTGGGCTCCTCTACAAAAACAAAAGTTAAAAAAAAACACACAAAAA2820.16390488866673256No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCTTAT207.837807E-444.00000425
ACACGCG207.837807E-444.00000436
CATGCGG207.837807E-444.0000042
TAGACGG207.837807E-444.0000042
TCGGGAT302.5151367E-644.0000045
CTTATGG302.5151367E-644.0000042
TCGTAGG207.837807E-444.0000042
TACTACC207.837807E-444.00000434
TGGGACG207.837807E-444.0000046
CTGTGCG207.837807E-444.0000041
GGGTCAA254.4270244E-544.07
GACCGAT254.4270244E-544.09
TATGCGG351.4365105E-744.02
TACGAGG254.4270244E-544.02
CCTCAAT351.4365105E-744.026
CGGGATC351.4365105E-744.06
ATGCGGG750.044.03
GCTACTA254.4270244E-544.032
AGGGCGA1300.042.3076936
CGTTTTT15850.041.3627781
Produced by FastQC (version 0.11.5)