##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546160_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	172051
Sequences flagged as poor quality	0
Sequence length	50
%GC	39
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.81714142899489	31.0	31.0	34.0	30.0	34.0
2	32.22683390390059	33.0	31.0	34.0	31.0	34.0
3	32.29484280823709	34.0	31.0	34.0	30.0	34.0
4	35.98447553341742	37.0	35.0	37.0	35.0	37.0
5	35.92217423903377	37.0	35.0	37.0	35.0	37.0
6	35.981825156494295	37.0	35.0	37.0	35.0	37.0
7	36.127235529000124	37.0	35.0	37.0	35.0	37.0
8	36.009125201248466	37.0	35.0	37.0	35.0	37.0
9	37.69001633236657	39.0	38.0	39.0	35.0	39.0
10	37.44599566407635	39.0	37.0	39.0	35.0	39.0
11	37.36483949526594	39.0	37.0	39.0	34.0	39.0
12	37.18459061557329	39.0	37.0	39.0	34.0	39.0
13	37.107305392005856	39.0	37.0	39.0	33.0	39.0
14	38.31927161132455	40.0	38.0	41.0	33.0	41.0
15	38.428448541420856	40.0	38.0	41.0	34.0	41.0
16	38.44992473162028	40.0	38.0	41.0	34.0	41.0
17	38.35734753067405	40.0	37.0	41.0	34.0	41.0
18	38.16312023760397	40.0	37.0	41.0	34.0	41.0
19	38.01049688755079	40.0	37.0	41.0	34.0	41.0
20	37.77252675078901	40.0	35.0	41.0	34.0	41.0
21	37.69106253378359	40.0	35.0	41.0	33.0	41.0
22	37.613905179278234	39.0	35.0	41.0	33.0	41.0
23	37.51793363595678	39.0	35.0	41.0	33.0	41.0
24	37.46189792561508	39.0	35.0	41.0	33.0	41.0
25	37.449058709336185	39.0	35.0	41.0	33.0	41.0
26	37.40909962743605	39.0	35.0	41.0	33.0	41.0
27	37.32543838745488	39.0	35.0	41.0	33.0	41.0
28	37.238313058337354	39.0	35.0	41.0	33.0	41.0
29	37.14256237975949	39.0	35.0	41.0	33.0	41.0
30	36.971886243032586	39.0	35.0	41.0	32.0	41.0
31	36.78313988294168	39.0	35.0	41.0	31.0	41.0
32	36.51124375911794	39.0	35.0	41.0	31.0	41.0
33	36.139958500677125	39.0	35.0	41.0	29.0	41.0
34	35.77676967875804	39.0	35.0	41.0	26.0	41.0
35	35.49596921842942	39.0	35.0	41.0	24.0	41.0
36	35.32871067299812	39.0	35.0	41.0	23.0	41.0
37	35.222625849312124	39.0	35.0	41.0	23.0	41.0
38	35.136773398585305	39.0	35.0	41.0	22.0	41.0
39	35.036425246002636	39.0	35.0	41.0	21.0	41.0
40	34.9402212134774	39.0	35.0	41.0	21.0	41.0
41	34.84537143056419	39.0	35.0	41.0	20.0	41.0
42	34.75717083887917	38.0	35.0	41.0	20.0	41.0
43	34.661083050955824	38.0	34.0	41.0	19.0	41.0
44	34.54286810306247	38.0	34.0	41.0	18.0	41.0
45	34.47780018715381	38.0	34.0	40.0	19.0	41.0
46	34.41519084457516	38.0	34.0	40.0	19.0	41.0
47	34.368518636915795	38.0	34.0	40.0	19.0	41.0
48	34.28994309826737	38.0	34.0	40.0	18.0	41.0
49	34.20977500857304	38.0	34.0	40.0	18.0	41.0
50	34.07371070205927	38.0	34.0	40.0	18.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	2.0
13	0.0
14	4.0
15	9.0
16	12.0
17	28.0
18	65.0
19	105.0
20	207.0
21	357.0
22	477.0
23	780.0
24	1247.0
25	2144.0
26	3376.0
27	4116.0
28	4145.0
29	3755.0
30	3606.0
31	3976.0
32	4330.0
33	5716.0
34	9125.0
35	14693.0
36	11356.0
37	14673.0
38	25659.0
39	58087.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.857995594329587	20.94437114576492	27.504635253500414	19.692998006405077
2	37.157586994553945	23.658682599926767	27.73189345019791	11.451836955321387
3	27.275633387774555	23.931276191361864	37.011118796170905	11.781971624692678
4	24.01148496666686	25.728417736601354	36.616468372749935	13.643628923981844
5	21.741809114739237	28.58745372011787	36.414783988468535	13.255953176674359
6	20.35849835223277	36.7786295923883	34.08756705860472	8.775304996774212
7	76.97252558834299	4.8433313378010014	15.940622257353922	2.2435208165020835
8	77.57525384914939	4.616654364112967	15.422752555928184	2.3853392308094694
9	70.61336464187944	7.423961499787854	18.07429192506873	3.8883819332639744
10	32.54674486053554	30.0300492295889	25.38723983005039	12.035966079825169
11	27.035007061859567	24.781605454196722	34.60601798303991	13.577369500903803
12	25.258789544960507	23.037355202817768	36.29970183259615	15.404153419625576
13	19.321596503362375	27.189031159365534	37.07621577323003	16.41315656404206
14	15.721501182788824	29.533684779513052	38.024190501653585	16.720623536044545
15	15.475062626779268	26.14282974234384	42.41416789207852	15.967939738798378
16	18.25853961906644	24.63862459387042	39.7300800344084	17.372755752654736
17	18.06964214099308	25.444780907986587	36.388628953042996	20.096947997977345
18	18.854874426768806	25.655764860419296	38.983789690266256	16.505571022545638
19	21.05654718659002	26.6537247676561	35.521444222933894	16.768283822819978
20	21.917338463595097	25.474423281468866	36.88731829515667	15.720919959779367
21	20.561345182533085	26.343351680606332	36.710045277272435	16.385257859588144
22	19.507006643379	24.606076105340858	36.32236952996495	19.564547721315193
23	17.523873735113426	27.431401154308897	36.22704895641409	18.81767615416359
24	17.188508058657025	25.473260835449953	40.8564902267351	16.481740879157925
25	16.926376481392143	28.80657479468297	37.756827917303596	16.51022080662129
26	17.059476550557683	29.252954065945563	35.727778391290954	17.9597909922058
27	16.188223259382394	28.957692777141663	36.55718362578538	18.29690033769057
28	15.80170995809382	27.429076262271067	39.342985510110374	17.426228269524735
29	16.894409215872038	25.70807493127038	38.55077854822117	18.846737304636417
30	17.508180713858103	28.60954019447722	37.326141667296326	16.556137424368355
31	19.71392203474551	29.153564931328503	34.72574992298795	16.406763110938037
32	20.430570005405375	27.7533987015478	34.20148676845819	17.61454452458864
33	20.217842383944294	28.674055948526888	33.198295854136276	17.90980581339254
34	17.583739705087446	28.017273947841048	35.297673364293146	19.101312982778364
35	19.361119668005415	29.129153564931325	33.73011490778897	17.779611859274286
36	20.17715677328234	30.975117842965165	31.978889980296536	16.86883540345595
37	18.547988677775777	31.728382863220787	33.13494254610552	16.58868591289792
38	18.04058099052025	30.799588494109305	33.34243916048148	17.817391354888954
39	18.343979401456544	31.43602768946417	31.99516422456132	18.224828684517963
40	19.734846063085946	29.81034693201434	32.56069421276249	17.894112792137214
41	18.1120714206834	29.10590464455307	32.37412162672696	20.407902308036572
42	17.98768969665971	29.609824993751854	31.219812729946355	21.182672579642084
43	18.505559398085452	29.309332697862843	32.07537299986632	20.109734904185387
44	17.68429128572342	29.88416225421532	32.728667662495425	19.702878797565837
45	17.59827028032386	32.90594068037965	29.72258225758641	19.773206781710073
46	18.126601995919813	32.20905429204131	31.566221643582427	18.098122068456448
47	17.698821860959832	30.29741181393889	33.29826621176279	18.705500113338484
48	17.761593945981133	29.581345066288485	34.02305130455505	18.634009683175336
49	18.212623001319376	29.689452546047395	33.170397149682366	18.92752730295087
50	17.542472871416035	30.675206770085616	33.252349594015726	18.529970764482627
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	259.0
1	516.5
2	774.0
3	2924.5
4	5075.0
5	3573.5
6	2072.0
7	2060.5
8	2049.0
9	2084.0
10	2119.0
11	2036.0
12	1953.0
13	1877.5
14	1802.0
15	1731.5
16	1661.0
17	1562.0
18	1463.0
19	1580.0
20	1697.0
21	1795.5
22	1894.0
23	1983.0
24	2072.0
25	2534.0
26	2996.0
27	3875.5
28	4755.0
29	5243.0
30	5731.0
31	6847.5
32	7964.0
33	8587.5
34	9211.0
35	10137.0
36	11063.0
37	10906.0
38	10749.0
39	10754.0
40	10759.0
41	10618.5
42	10478.0
43	10244.5
44	10011.0
45	9558.5
46	9106.0
47	8863.5
48	8621.0
49	8506.0
50	8391.0
51	7828.0
52	7265.0
53	6575.5
54	5886.0
55	5378.5
56	4871.0
57	4510.5
58	4150.0
59	3990.5
60	3831.0
61	3484.5
62	3138.0
63	2824.5
64	2511.0
65	2172.5
66	1834.0
67	1577.0
68	1320.0
69	1128.5
70	937.0
71	819.0
72	701.0
73	546.5
74	392.0
75	319.0
76	246.0
77	185.0
78	124.0
79	96.0
80	68.0
81	52.0
82	36.0
83	23.5
84	11.0
85	6.5
86	2.0
87	1.5
88	1.0
89	1.0
90	1.0
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	172051.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	44.20317231518561
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.30058381107663	31.075088200591683
2	11.106874243938359	9.819181521758082
3	5.79866407195077	7.689580415109473
4	3.5909640772103297	6.349280155302788
5	2.147215063377689	4.7456858722123085
6	1.4766212591384842	3.916280637717886
7	0.9966864776731709	3.0839692881761804
8	0.8033976752748119	2.841018070223364
9	0.540419712828065	2.1499439119795873
>10	3.1728291169200022	24.5787586238964
>50	0.05654026192605059	1.5640711184474372
>100	0.007889338873402409	0.5231007085108486
>500	0.0	0.0
>1k	0.0013148898122337348	1.6640414760739546
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2863	1.6640414760739546	No Hit
GGCTCTGGGCTCCTCTACAAAAACAAAAGTTAAAAAAAAACACACAAAAA	282	0.16390488866673256	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.018599136302607948	0.0
2	0.0	0.0	0.0	0.10810747975890869	0.0
3	0.0	0.0	0.0	0.18773503205444897	0.0
4	0.0	0.0	0.0	0.30281718792683565	0.0
5	0.0	0.0	0.0	0.5509994129647604	0.0
6	0.0	0.0	0.0	0.8387048026457271	0.0
7	0.0	0.0	0.0	1.0444577479933275	0.0
8	0.0	0.0	0.0	1.4455016245183114	0.0
9	0.0	0.0	0.0	1.6204497503647175	0.0
10	0.0	0.0	0.0	1.9226857152820966	0.0
11	0.0	0.0	0.0	2.2138784430198024	0.0
12	0.0	0.0	0.0	2.4731039052374006	0.0
13	0.0	0.0	0.0	2.61259742750696	0.0
14	0.0	0.0	0.0	2.6573515992351107	0.0
15	0.0	0.0	0.0	2.7439538276441287	0.0
16	0.0	0.0	0.0	2.8787975658380365	0.0
17	0.0	0.0	0.0	3.0095727429657484	0.0
18	0.0	0.0	0.0	3.201376336086393	0.0
19	0.0	0.0	0.0	3.3077401468169323	0.0
20	0.0	0.0	0.0	3.427472086764971	0.0
21	0.0	0.0	0.0	3.564059493987248	0.0
22	0.0	0.0	0.0	3.733195389739089	0.0
23	0.0	0.0	0.0	3.9063998465571257	0.0
24	0.0	0.0	0.0	4.046474591836142	0.0
25	0.0	0.0	0.0	4.171437538869289	0.0
26	0.0	0.0	0.0	4.302793939006458	0.0
27	0.0	0.0	0.0	4.442868684285473	0.0
28	0.0	0.0	0.0	4.564925516271338	0.0
29	0.0	0.0	0.0	4.686401125247746	0.0
30	0.0	0.0	0.0	4.867161481188718	0.0
31	0.0	0.0	0.0	5.027579031798711	0.0
32	0.0	0.0	0.0	5.166491331058814	0.0
33	0.0	0.0	0.0	5.319934205555329	0.0
34	0.0	0.0	0.0	5.464077511900541	0.0
35	0.0	0.0	0.0	5.662274558125207	0.0
36	0.0	0.0	0.0	5.83431656892433	0.0
37	0.0	0.0	0.0	5.999383903609976	0.0
38	0.0	0.0	0.0	6.173169583437469	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCCTTAT	20	7.837807E-4	44.000004	25
ACACGCG	20	7.837807E-4	44.000004	36
CATGCGG	20	7.837807E-4	44.000004	2
TAGACGG	20	7.837807E-4	44.000004	2
TCGGGAT	30	2.5151367E-6	44.000004	5
CTTATGG	30	2.5151367E-6	44.000004	2
TCGTAGG	20	7.837807E-4	44.000004	2
TACTACC	20	7.837807E-4	44.000004	34
TGGGACG	20	7.837807E-4	44.000004	6
CTGTGCG	20	7.837807E-4	44.000004	1
GGGTCAA	25	4.4270244E-5	44.0	7
GACCGAT	25	4.4270244E-5	44.0	9
TATGCGG	35	1.4365105E-7	44.0	2
TACGAGG	25	4.4270244E-5	44.0	2
CCTCAAT	35	1.4365105E-7	44.0	26
CGGGATC	35	1.4365105E-7	44.0	6
ATGCGGG	75	0.0	44.0	3
GCTACTA	25	4.4270244E-5	44.0	32
AGGGCGA	130	0.0	42.307693	6
CGTTTTT	1585	0.0	41.362778	1
>>END_MODULE