FastQCFastQC Report
Thu 26 May 2016
SRR1546159_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546159_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1557221
Sequences flagged as poor quality0
Sequence length50
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT313032.0101835256524283No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCC20070.1288834404365212No Hit
GAATCTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCCGTCTT17360.11148064404474381No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAAGCG207.857343E-444.0000041
TATCTCG600.044.00000425
CCCGATA207.857343E-444.00000416
CGTTCGA351.4465331E-744.014
TAGCCGT454.802132E-1044.044
TAAGACG454.802132E-1044.01
CGTTTTT177500.042.2523961
TATCGTA603.6379788E-1240.33333641
CGGTCTA1100.040.031
TACCGGT557.8216544E-1140.037
ACGGGAT7200.039.722225
CTAGACG1000.039.61
TAGTTCG501.3478711E-939.61
ATAACGG1400.039.2857132
GGCGATA3550.039.0422558
CGGGCAC1250.038.726
ACGGGTA2050.038.6341485
CGATTCA404.1257954E-738.50000410
ACGATAC404.1257954E-738.50000437
GTTCGAA404.1257954E-738.50000415