FastQCFastQC Report
Thu 26 May 2016
SRR1546158_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546158_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1600879
Sequences flagged as poor quality0
Sequence length50
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT321602.0088963625608183No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCC20500.12805464997666907No Hit
GAATCTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCCGTCTT17610.1100020676141045No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAACGTA408.3164196E-944.00000422
CGCATCG207.857409E-444.00000421
GTTACGA207.857409E-444.00000430
TATTACG800.044.0000041
CACGTTA408.3164196E-944.00000423
ACGTAAG302.5283425E-644.01
CCGAAAC254.443626E-544.042
CGTTTTT182000.042.10221
TCGATAG850.041.4117621
CGTTGAT650.040.61538325
TTTACGG1700.040.1176452
GCTAGCG557.8216544E-1140.01
ACGGGAT7100.039.6619725
TAGTCGG501.3478711E-939.6000022
ATGGGAC8200.038.6341485
GTCGATT404.1258863E-738.50000435
CACGGGA3500.038.3428574
GCCCTAC19850.037.90427826
ATAGCCG357.2919993E-637.7142871
AGCGGTA700.037.7142874
Produced by FastQC (version 0.11.5)