##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546158_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1600879
Sequences flagged as poor quality	0
Sequence length	50
%GC	38
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.811098152952223	31.0	31.0	34.0	30.0	34.0
2	32.19990205380919	33.0	31.0	34.0	30.0	34.0
3	32.227784860692154	33.0	31.0	34.0	30.0	34.0
4	35.9461077320647	37.0	35.0	37.0	35.0	37.0
5	35.865499516203286	37.0	35.0	37.0	35.0	37.0
6	35.93526181554009	37.0	35.0	37.0	35.0	37.0
7	36.13173075541624	37.0	35.0	37.0	35.0	37.0
8	36.01352694363534	37.0	35.0	37.0	35.0	37.0
9	37.690704294328306	39.0	38.0	39.0	35.0	39.0
10	37.436537677113634	39.0	37.0	39.0	34.0	39.0
11	37.364298613449236	39.0	37.0	39.0	34.0	39.0
12	37.139739480622836	39.0	37.0	39.0	34.0	39.0
13	37.04219556880938	39.0	37.0	39.0	33.0	39.0
14	38.22455725885592	40.0	37.0	41.0	33.0	41.0
15	38.33062523776	40.0	37.0	41.0	33.0	41.0
16	38.36598456223113	40.0	37.0	41.0	34.0	41.0
17	38.27929156419692	40.0	37.0	41.0	34.0	41.0
18	38.073049868228644	39.0	37.0	41.0	34.0	41.0
19	37.86645773977921	39.0	37.0	41.0	34.0	41.0
20	37.58733858086713	39.0	35.0	41.0	33.0	41.0
21	37.50849377123443	39.0	35.0	41.0	33.0	41.0
22	37.429076775946214	39.0	35.0	41.0	33.0	41.0
23	37.36662233685369	39.0	35.0	41.0	33.0	41.0
24	37.30054238952476	39.0	35.0	41.0	33.0	41.0
25	37.26449531788474	39.0	35.0	41.0	33.0	41.0
26	37.23251726082983	39.0	35.0	41.0	33.0	41.0
27	37.149282363001824	39.0	35.0	41.0	33.0	41.0
28	37.06407667287784	39.0	35.0	41.0	33.0	41.0
29	36.98456035715379	39.0	35.0	41.0	32.0	41.0
30	36.79949702632116	39.0	35.0	41.0	32.0	41.0
31	36.55041011844118	39.0	35.0	41.0	31.0	41.0
32	36.237959271125426	39.0	35.0	41.0	30.0	41.0
33	35.81123995005244	39.0	35.0	41.0	27.0	41.0
34	35.376347619026795	39.0	35.0	41.0	23.0	41.0
35	35.08192061985947	39.0	35.0	41.0	21.0	41.0
36	34.92411981167846	39.0	35.0	41.0	21.0	41.0
37	34.81270414565998	39.0	35.0	41.0	20.0	41.0
38	34.729962726727	39.0	35.0	41.0	18.0	41.0
39	34.6164682027811	39.0	34.0	41.0	18.0	41.0
40	34.529396038051594	39.0	34.0	41.0	18.0	41.0
41	34.39793825766969	38.0	34.0	41.0	18.0	41.0
42	34.337194753632225	38.0	34.0	41.0	17.0	41.0
43	34.2542915485805	38.0	34.0	41.0	17.0	41.0
44	34.13693789474407	38.0	34.0	40.0	15.0	41.0
45	34.07843066215498	38.0	34.0	40.0	17.0	41.0
46	34.03497828380534	38.0	34.0	40.0	17.0	41.0
47	33.967295467052786	38.0	34.0	40.0	16.0	41.0
48	33.898966130482066	38.0	34.0	40.0	15.0	41.0
49	33.81483047750642	38.0	33.0	40.0	15.0	41.0
50	33.645518493277756	38.0	33.0	40.0	15.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	3.0
10	3.0
11	6.0
12	4.0
13	11.0
14	14.0
15	25.0
16	83.0
17	225.0
18	610.0
19	1285.0
20	2226.0
21	3751.0
22	5906.0
23	8940.0
24	13812.0
25	22523.0
26	35759.0
27	43978.0
28	41608.0
29	36827.0
30	35069.0
31	37263.0
32	42731.0
33	54189.0
34	89000.0
35	137405.0
36	102816.0
37	130708.0
38	225387.0
39	528711.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.93152012113345	20.741480149342955	26.63393048444011	20.693069245083482
2	37.40201476813675	24.121997977361186	27.37639759157313	11.099589662928928
3	26.12208667863093	24.13199248662766	38.219128366353736	11.52679246838768
4	23.151343730538034	25.703004411951184	38.76482857230309	12.380823285207688
5	21.77247624586243	29.004940410861785	37.452674436981184	11.769908906294605
6	19.562065590216374	36.72632347604035	34.64552911244385	9.066081821299424
7	75.1452795620406	4.999066138040414	17.75518324620412	2.1004710537148656
8	75.24060219416958	4.785558433835411	17.320047299015105	2.6537920729799067
9	69.04756699288328	7.561720779646682	19.26666537570922	4.124046851760814
10	31.362707612505382	31.979243902880857	26.22334355063687	10.434704933976896
11	27.036022085366852	24.220318962270103	35.6093121341463	13.134346818216741
12	24.430703382329334	23.311068481752837	37.831716200912126	14.426511935005706
13	19.095884198618386	28.508213300318136	38.032918165582785	14.362984335480695
14	15.691629411092281	30.219023424006437	38.696241252461924	15.393105912439353
15	14.909059335527544	25.813693602077358	45.2348990773194	14.042347985075699
16	17.426114028605536	24.497229334634284	41.67779076369919	16.398865873060984
17	17.55710456567923	24.51859259819137	38.01224202453777	19.91206081159163
18	18.553931933643955	25.63529161167084	40.342586791381486	15.468189663303722
19	20.898081616411986	26.27999992504118	36.50913029654333	16.3127881620035
20	21.702639612362958	26.214348492297045	37.17426488822703	14.908747007112968
21	19.524336317735443	25.97491752968213	38.291151298755246	16.209594853827177
22	18.792863170795542	24.500914809926297	37.52244860479774	19.18377341448042
23	17.02933201072661	26.823951091868903	37.78861487970047	18.358102017704024
24	16.204847461925603	25.693447162465123	42.17214417829205	15.929561197317224
25	16.26937451237726	28.784873809950657	38.877579129965476	16.068172547706602
26	17.00497039438958	29.04454365383018	36.731882921819825	17.218603029960416
27	16.417543112252705	29.230753854601126	37.01329082335392	17.338412209792246
28	14.930360133401713	27.864067178094032	40.509057836351154	16.6965148521531
29	16.60356591597491	26.225030124075587	38.855841072310895	18.315562887638603
30	17.228909867641466	28.861519202887916	37.484094675487654	16.425476253982968
31	19.53782890524518	28.774754369318355	35.6060639186347	16.081352806801764
32	21.024012433169528	27.584095987266995	34.34194589347477	17.049945686088705
33	20.2633053466252	28.86089454605876	33.76451312060437	17.111286986711676
34	17.215479745814644	28.33018610400911	35.837686670885184	18.616647479291064
35	18.23298325482438	29.230816320284042	35.52367168286922	17.01252874202235
36	20.603805784197306	30.2140261693732	33.16265626571403	16.01951178071547
37	17.373892717688218	32.3801486558322	34.31558537528445	15.930373251195123
38	17.609888067742784	31.28450057749524	34.25736735880726	16.84824399595472
39	17.874305303523876	31.320793139269114	33.72509727468472	17.07980428252229
40	19.455436669479706	28.656631762925244	34.188780038966094	17.69915152862896
41	16.820946492520672	29.428145412613944	33.70054826129895	20.050359833566432
42	18.218928476168404	29.303651306563456	32.75687918949527	19.720541027772867
43	18.194504394148463	29.28784748878585	33.44212773107774	19.07552038598795
44	18.1635214154224	29.954418791176597	32.71790060335603	19.16415919004497
45	17.266076948976156	32.114544572075715	31.884358530532285	18.73501994841584
46	18.503022402067863	31.579088738124494	32.354162931739374	17.563725928068266
47	17.2330950683968	30.927009474170124	33.66194446925721	18.17795098817587
48	18.10168038933611	28.86339317337538	34.30471634645716	18.73021009083135
49	18.180512081175404	28.88600575059077	34.257617221538915	18.675864946694908
50	17.629252429446574	30.692513300505535	33.65501077845359	18.023223491594305
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2589.0
1	4727.0
2	6865.0
3	29591.0
4	52317.0
5	36122.5
6	19928.0
7	19975.5
8	20023.0
9	20399.5
10	20776.0
11	20847.0
12	20918.0
13	20393.5
14	19869.0
15	18172.5
16	16476.0
17	16186.5
18	15897.0
19	16551.5
20	17206.0
21	18257.0
22	19308.0
23	21713.0
24	24118.0
25	27132.5
26	30147.0
27	35158.5
28	40170.0
29	48680.5
30	57191.0
31	61061.0
32	64931.0
33	77975.5
34	91020.0
35	94846.0
36	98672.0
37	97046.5
38	95421.0
39	99043.5
40	102666.0
41	104528.5
42	106391.0
43	109821.0
44	113251.0
45	110068.5
46	106886.0
47	98256.0
48	89626.0
49	81060.0
50	72494.0
51	63016.5
52	53539.0
53	48573.0
54	43607.0
55	40702.0
56	37797.0
57	35659.0
58	33521.0
59	30526.0
60	27531.0
61	25031.0
62	22531.0
63	19890.5
64	17250.0
65	15355.5
66	13461.0
67	11861.0
68	10261.0
69	8446.0
70	6631.0
71	5452.0
72	4273.0
73	3344.5
74	2416.0
75	1937.5
76	1459.0
77	1084.0
78	709.0
79	532.5
80	356.0
81	289.5
82	223.0
83	148.5
84	74.0
85	54.0
86	34.0
87	32.5
88	31.0
89	21.5
90	12.0
91	7.5
92	3.0
93	2.5
94	2.0
95	1.5
96	1.0
97	1.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1600879.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	25.425511267140276
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.09459847274404	19.855950933715494
2	7.30974383294785	3.717079483690494
3	2.8509457345189784	2.1746025868505336
4	1.6548449564403644	1.6830111634137896
5	1.134132376967766	1.4417947764511259
6	0.8579622401006043	1.3088477161475294
7	0.651846062689677	1.1601463587950187
8	0.550344781922926	1.1194237962874571
9	0.47557639522760814	1.0882595695720956
>10	5.217814904042896	28.552284887515032
>50	0.7152411900271116	12.629117169263775
>100	0.4805190496673017	21.922229292978788
>500	0.004946155925344171	0.833842020746418
>1k	0.0012365389813360427	0.4912128697738495
>5k	0.0	0.0
>10k+	2.473077962672085E-4	2.022197374798643
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	32160	2.0088963625608183	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCC	2050	0.12805464997666907	No Hit
GAATCTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCCGTCTT	1761	0.1100020676141045	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.05059720316151314	0.0
2	0.0	0.0	0.0	0.19501786206202967	0.0
3	0.0	0.0	0.0	0.2940884351659307	0.0
4	0.0	0.0	0.0	0.47467672447449183	0.0
5	0.0	0.0	0.0	0.9234926562219881	0.0
6	0.0	0.0	0.0	1.3342045213910607	0.0
7	0.0	0.0	0.0	1.6215466628021231	0.0
8	0.0	0.0	0.0	2.223153654960806	0.0
9	0.0	0.0	0.0	2.504186762397408	0.0
10	0.0	0.0	0.0	3.052635458395044	0.0
11	0.0	0.0	0.0	3.425618051083186	0.0
12	0.0	0.0	0.0	3.8106565205740095	0.0
13	6.246568291544833E-5	0.0	0.0	3.955327042206188	0.0
14	6.246568291544833E-5	0.0	0.0	4.017355465341228	0.0
15	6.246568291544833E-5	0.0	0.0	4.118300008932593	0.0
16	6.246568291544833E-5	0.0	0.0	4.2886439262430205	0.0
17	6.246568291544833E-5	0.0	0.0	4.481725352134671	0.0
18	6.246568291544833E-5	0.0	0.0	4.746642313379088	0.0
19	6.246568291544833E-5	0.0	0.0	4.8972470748882335	0.0
20	6.246568291544833E-5	0.0	0.0	5.062468806199594	0.0
21	6.246568291544833E-5	0.0	0.0	5.266106932503956	0.0
22	6.246568291544833E-5	0.0	0.0	5.490358734170415	0.0
23	6.246568291544833E-5	0.0	0.0	5.722918471664629	0.0
24	6.246568291544833E-5	0.0	0.0	5.908191687191849	0.0
25	6.246568291544833E-5	0.0	0.0	6.0742879380640264	0.0
26	6.246568291544833E-5	0.0	0.0	6.242570487838244	0.0
27	6.246568291544833E-5	0.0	0.0	6.425220144683014	0.0
28	6.246568291544833E-5	0.0	0.0	6.608869252454433	0.0
29	6.246568291544833E-5	0.0	0.0	6.834557764827948	0.0
30	6.246568291544833E-5	0.0	0.0	7.088668162927991	0.0
31	6.246568291544833E-5	0.0	0.0	7.305361616961681	0.0
32	6.246568291544833E-5	0.0	0.0	7.519556443678754	0.0
33	6.246568291544833E-5	0.0	0.0	7.722882241568538	0.0
34	6.246568291544833E-5	0.0	0.0	7.940200352431383	0.0
35	6.246568291544833E-5	0.0	0.0	8.203180877505421	0.0
36	6.246568291544833E-5	0.0	0.0	8.418687483563717	0.0
37	6.246568291544833E-5	0.0	0.0	8.640503123596474	0.0
38	6.246568291544833E-5	0.0	0.0	8.884306684015469	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CAACGTA	40	8.3164196E-9	44.000004	22
CGCATCG	20	7.857409E-4	44.000004	21
GTTACGA	20	7.857409E-4	44.000004	30
TATTACG	80	0.0	44.000004	1
CACGTTA	40	8.3164196E-9	44.000004	23
ACGTAAG	30	2.5283425E-6	44.0	1
CCGAAAC	25	4.443626E-5	44.0	42
CGTTTTT	18200	0.0	42.1022	1
TCGATAG	85	0.0	41.411762	1
CGTTGAT	65	0.0	40.615383	25
TTTACGG	170	0.0	40.117645	2
GCTAGCG	55	7.8216544E-11	40.0	1
ACGGGAT	710	0.0	39.661972	5
TAGTCGG	50	1.3478711E-9	39.600002	2
ATGGGAC	820	0.0	38.634148	5
GTCGATT	40	4.1258863E-7	38.500004	35
CACGGGA	350	0.0	38.342857	4
GCCCTAC	1985	0.0	37.904278	26
ATAGCCG	35	7.2919993E-6	37.714287	1
AGCGGTA	70	0.0	37.714287	4
>>END_MODULE