FastQCFastQC Report
Thu 26 May 2016
SRR1546157_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546157_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1762702
Sequences flagged as poor quality0
Sequence length50
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT235531.336187285201923No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA39760.2255628007456734No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCA30880.17518559575016082No Hit
GAAGAAGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTAT19280.10937753517043719No Hit
TGTGTTGGGTTAACAGAGAAGTTATAGGTGGATTATTTATAGTGTGATTA18990.10773233365594412No Hit
TAGTATGGGGTCTATTTAAGAAGCAAAACTCAAAGCTTAAAAATTTCTCT17640.10007363695054523No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTTGCG207.8576274E-444.01
AACCGCA207.8576274E-444.043
CGGCGAA207.8576274E-444.031
ATCTCGA254.4438104E-544.034
CCTAGCG207.8576274E-444.01
CCGGATG207.8576274E-444.01
CCGGATC254.4438104E-544.039
GATCCGG1450.044.02
GCCGGAT207.8576274E-444.019
CGTCTGG502.7284841E-1144.02
GTTAGCG1600.044.01
TTCCGTA207.8576274E-444.042
CACTGCG551.8189894E-1244.01
TACGGAC302.5284899E-644.017
TCGATAG302.5284899E-644.01
TGGCCGG207.8576274E-444.011
ACGATGA207.8576274E-444.030
GTAGCGC207.8576274E-444.09
ACCTACG207.8576274E-444.035
TACGATC207.8576274E-444.018
Produced by FastQC (version 0.11.5)