##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546157_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1762702
Sequences flagged as poor quality	0
Sequence length	50
%GC	37
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.865355006121284	31.0	31.0	34.0	30.0	34.0
2	32.22167048088673	33.0	31.0	34.0	30.0	34.0
3	32.14074415301055	33.0	31.0	34.0	30.0	34.0
4	35.88865673267518	37.0	35.0	37.0	35.0	37.0
5	35.871538127261445	37.0	35.0	37.0	35.0	37.0
6	35.925863248580875	37.0	35.0	37.0	35.0	37.0
7	36.16092396786297	37.0	35.0	37.0	35.0	37.0
8	36.075389373813614	37.0	35.0	37.0	35.0	37.0
9	37.8159694605214	39.0	38.0	39.0	35.0	39.0
10	37.45696323031346	39.0	37.0	39.0	35.0	39.0
11	37.277574428349205	39.0	37.0	39.0	34.0	39.0
12	36.81249297952802	39.0	35.0	39.0	33.0	39.0
13	36.59554990009656	39.0	35.0	39.0	33.0	39.0
14	37.77557068636673	40.0	35.0	41.0	33.0	41.0
15	37.94066836027871	40.0	35.0	41.0	33.0	41.0
16	38.04993753907354	40.0	35.0	41.0	34.0	41.0
17	37.97521021704179	40.0	35.0	41.0	34.0	41.0
18	37.78563534845935	39.0	36.0	41.0	34.0	41.0
19	37.55369313701352	39.0	36.0	41.0	34.0	41.0
20	37.29229274148438	38.0	35.0	41.0	34.0	41.0
21	37.20766981599839	38.0	35.0	41.0	33.0	41.0
22	37.09882952421907	38.0	35.0	41.0	33.0	41.0
23	37.04146758782823	38.0	35.0	41.0	33.0	41.0
24	36.993934312209326	38.0	35.0	41.0	33.0	41.0
25	36.97060705666642	38.0	35.0	41.0	33.0	41.0
26	36.91151538944189	38.0	35.0	41.0	33.0	41.0
27	36.845159873875446	38.0	35.0	41.0	33.0	41.0
28	36.7918530755624	38.0	35.0	41.0	33.0	41.0
29	36.71391250477959	38.0	35.0	41.0	32.0	41.0
30	36.5319146401377	38.0	35.0	41.0	32.0	41.0
31	36.24599450162307	38.0	35.0	41.0	31.0	41.0
32	35.874944829018176	38.0	35.0	41.0	30.0	41.0
33	35.41305280189164	38.0	35.0	41.0	26.0	41.0
34	34.90168729598083	38.0	35.0	41.0	23.0	41.0
35	34.63800063765741	38.0	35.0	41.0	21.0	41.0
36	34.449058320691755	38.0	34.0	41.0	18.0	41.0
37	34.39841674883219	38.0	34.0	41.0	18.0	41.0
38	34.382878104183234	38.0	34.0	41.0	18.0	41.0
39	34.317034303018886	38.0	34.0	41.0	18.0	41.0
40	34.17690681692084	38.0	34.0	40.0	18.0	41.0
41	34.07439544517451	38.0	34.0	40.0	17.0	41.0
42	34.02349574687043	38.0	34.0	40.0	16.0	41.0
43	33.89273399587678	38.0	34.0	40.0	15.0	41.0
44	33.761449751574574	38.0	34.0	40.0	15.0	41.0
45	33.711657444083	37.0	34.0	40.0	15.0	41.0
46	33.65300714471306	37.0	34.0	40.0	15.0	41.0
47	33.60589594837925	37.0	33.0	40.0	15.0	41.0
48	33.55213416675082	37.0	33.0	40.0	13.0	41.0
49	33.44568282103271	37.0	33.0	40.0	12.0	41.0
50	33.30901763315637	37.0	33.0	40.0	11.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	0.0
9	2.0
10	0.0
11	2.0
12	5.0
13	5.0
14	17.0
15	31.0
16	80.0
17	271.0
18	613.0
19	1370.0
20	2450.0
21	3984.0
22	6673.0
23	10401.0
24	16626.0
25	28050.0
26	44439.0
27	52525.0
28	46574.0
29	39152.0
30	38061.0
31	40874.0
32	48568.0
33	62899.0
34	111062.0
35	234394.0
36	97305.0
37	120244.0
38	206714.0
39	549310.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	25.039229546457655	23.6823354146078	31.02021782468052	20.258217214254024
2	33.25014664985913	26.413313197579626	29.60727337916449	10.72926677339675
3	23.34365082696905	24.317213005942016	41.88138437467025	10.457751792418685
4	21.571485140426457	26.3713321934167	40.501003572923835	11.556179093233004
5	20.07940082895464	30.394757593739612	39.120339115743896	10.405502461561852
6	17.52610480954807	38.011927143669205	35.325710188108935	9.136257858673787
7	78.13969689714995	5.165081789207705	15.162631006261979	1.532590307380374
8	79.96150228456086	3.672089780348579	14.709065968042246	1.6573419670483158
9	75.22542097302892	5.9271504769382455	16.28800557326196	2.559422976770889
10	37.899713054163435	33.36894154542288	22.21135506739086	6.519990333022825
11	33.758060069143845	20.351766776233305	35.56477498749079	10.32539816713205
12	33.555757013947904	20.72636214175737	33.67284997691045	12.045030867384277
13	16.947504456226863	37.936815184869594	32.21860530027197	12.897075058631577
14	11.490371032653279	38.684984756357	34.41506278429366	15.409581426696061
15	10.433470887308234	26.716143738419767	50.19906938325367	12.651315991018333
16	11.329878788360142	23.20579428627187	46.748117378887635	18.716209546480346
17	12.142551605433024	25.725732426694925	35.2614338668703	26.87028210100176
18	14.63140111034083	28.53828951235092	40.73172890255982	16.098580474748424
19	18.151621771575684	28.273638992864363	33.76628607671631	19.80845315884364
20	20.66066754335106	24.832104348891644	39.28151213307751	15.225715974679781
21	16.10629590254053	29.842480464650293	37.69457344463216	16.356650188177017
22	19.511976499714642	26.279030715344966	32.04098026779342	22.16801251714697
23	14.021144810637306	30.17883907773407	33.61186405870079	22.188152052927833
24	12.52968454111926	26.568869837329284	48.55596691896872	12.345478702582739
25	11.334303813123261	32.419319885040125	41.22086433214463	15.025511969691985
26	13.059779815306275	32.14962029883667	35.979592693489884	18.811007192367175
27	12.553114479929109	36.53096212519189	34.064918517140164	16.851004877738834
28	10.192023382284697	31.971257762230938	41.890120962023076	15.94659789346129
29	12.03209617961516	24.823197568278697	39.49987008581144	23.6448361662947
30	13.162179426811793	35.38476724937057	35.40848084361395	16.044572480203687
31	18.247837694630174	34.68771238700586	31.802936627972283	15.261513290391681
32	20.221966049848472	28.431805262602527	33.051701308559245	18.294527378989756
33	19.910455652742208	33.46186706544839	29.460396595680947	17.167280686128457
34	15.412588174291514	28.16942398658423	34.6429515595943	21.77503627952995
35	18.218791378236364	28.041211730627186	33.849283656568154	19.890713234568295
36	23.72811740158007	31.085231650046346	30.340295750501216	14.846355197872358
37	15.106353768248972	33.76940628648518	36.399232541859035	14.725007403406815
38	15.291921152866452	31.375978469417976	35.78472141065251	17.547378967063064
39	16.48559994826125	32.371382116773	35.00915072428578	16.133867210679966
40	18.899791343063093	28.543508772327936	32.34477523710758	20.21192464750139
41	14.471192521481226	29.282374445595455	33.917871540396504	22.328561492526813
42	16.23501873827794	28.38426461194235	31.747907473866828	23.63280917591289
43	17.396928125117007	30.33893420442026	30.916173011660508	21.347964658802226
44	16.062953352296645	31.487568516969972	30.365654546259098	22.083823584474292
45	15.324654989896194	39.25042349756227	26.380692822723294	19.044228689818247
46	18.668215047126512	36.085452901284505	29.058740501797807	16.187591549791172
47	16.454568043832708	30.99758212108456	33.13702486296606	19.41082497211667
48	16.968721882655153	28.118252546374826	35.852231403833436	19.06079416713659
49	18.400898166564737	30.66230139864821	32.94385551272988	17.99294492205716
50	16.793139169298044	32.477923097608105	32.05459572860302	18.674342004490832
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	4747.0
1	5516.5
2	6286.0
3	20947.5
4	35609.0
5	27293.0
6	18977.0
7	20511.5
8	22046.0
9	24196.0
10	26346.0
11	26382.5
12	26419.0
13	25484.5
14	24550.0
15	22451.5
16	20353.0
17	19075.5
18	17798.0
19	16175.0
20	14552.0
21	14549.0
22	14546.0
23	15411.5
24	16277.0
25	21832.5
26	27388.0
27	30837.0
28	34286.0
29	41994.0
30	49702.0
31	64617.5
32	79533.0
33	102815.5
34	126098.0
35	126541.5
36	126985.0
37	123714.5
38	120444.0
39	130373.0
40	140302.0
41	148735.5
42	157169.0
43	159435.5
44	161702.0
45	141476.5
46	121251.0
47	112898.5
48	104546.0
49	98077.5
50	91609.0
51	81283.5
52	70958.0
53	56202.0
54	41446.0
55	31983.0
56	22520.0
57	18094.0
58	13668.0
59	11274.5
60	8881.0
61	7480.0
62	6079.0
63	4970.5
64	3862.0
65	3193.0
66	2524.0
67	1917.0
68	1310.0
69	1028.5
70	747.0
71	651.5
72	556.0
73	405.5
74	255.0
75	237.0
76	219.0
77	140.5
78	62.0
79	55.0
80	48.0
81	31.5
82	15.0
83	18.5
84	22.0
85	13.5
86	5.0
87	4.0
88	3.0
89	1.5
90	0.0
91	0.0
92	0.0
93	0.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1762702.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	22.326766130552496
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.38983354450136	17.501914805610213
2	8.9857325074364	4.012446964104711
3	3.3352407775779733	2.233954224901964
4	1.7505066810438852	1.5633261311054591
5	1.0324309494974548	1.1525422177686964
6	0.7264918562712122	0.9732128262430988
7	0.5488543441135252	0.8577899806532322
8	0.4305175819422911	0.7689652293693203
9	0.3505488319861218	0.7043959610183234
>10	3.044360082119524	14.70412354325886
>50	0.7050453402810568	11.099421759522583
>100	0.6145444371559184	27.591058836017694
>500	0.06902121380887472	10.731666753347971
>1k	0.016616218139173543	4.760897218661694
>5k	0.0	0.0
>10k+	2.5563412521805454E-4	1.3442835484162186
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	23553	1.336187285201923	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3976	0.2255628007456734	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCA	3088	0.17518559575016082	No Hit
GAAGAAGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTAT	1928	0.10937753517043719	No Hit
TGTGTTGGGTTAACAGAGAAGTTATAGGTGGATTATTTATAGTGTGATTA	1899	0.10773233365594412	No Hit
TAGTATGGGGTCTATTTAAGAAGCAAAACTCAAAGCTTAAAAATTTCTCT	1764	0.10007363695054523	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	5.67310867066583E-5	0.0	0.0	0.06558113623289699	0.0
2	5.67310867066583E-5	0.0	0.0	0.2713447877179467	0.0
3	5.67310867066583E-5	0.0	0.0	0.3919550780563022	0.0
4	5.67310867066583E-5	0.0	0.0	0.6334025830798399	0.0
5	5.67310867066583E-5	0.0	0.0	1.151073749278097	0.0
6	5.67310867066583E-5	0.0	0.0	1.5686712785258086	0.0
7	5.67310867066583E-5	0.0	0.0	1.8669066013427114	0.0
8	5.67310867066583E-5	0.0	0.0	2.569123992597728	0.0
9	5.67310867066583E-5	0.0	0.0	2.82730716819973	0.0
10	5.67310867066583E-5	0.0	0.0	3.361997660409984	0.0
11	5.67310867066583E-5	0.0	0.0	3.857316778445818	0.0
12	5.67310867066583E-5	0.0	0.0	4.316271269902684	0.0
13	5.67310867066583E-5	0.0	0.0	4.465417296854488	0.0
14	5.67310867066583E-5	0.0	0.0	4.518744518358747	0.0
15	5.67310867066583E-5	0.0	0.0	4.608379635355267	0.0
16	5.67310867066583E-5	0.0	0.0	4.815561564007983	0.0
17	5.67310867066583E-5	0.0	0.0	5.092976578003542	0.0
18	5.67310867066583E-5	0.0	0.0	5.4679690611345535	0.0
19	5.67310867066583E-5	0.0	0.0	5.6659038226540845	0.0
20	5.67310867066583E-5	0.0	0.0	5.851754862705097	0.0
21	5.67310867066583E-5	0.0	0.0	6.140289169695161	0.0
22	5.67310867066583E-5	0.0	0.0	6.428880207771932	0.0
23	5.67310867066583E-5	0.0	0.0	6.769890769965655	0.0
24	5.67310867066583E-5	0.0	0.0	7.0223441058102845	0.0
25	5.67310867066583E-5	0.0	0.0	7.239737630070199	0.0
26	5.67310867066583E-5	0.0	0.0	7.472902396434565	0.0
27	5.67310867066583E-5	0.0	0.0	7.687118979838907	0.0
28	5.67310867066583E-5	0.0	0.0	7.924368384446152	0.0
29	5.67310867066583E-5	0.0	0.0	8.187430433504925	0.0
30	5.67310867066583E-5	0.0	0.0	8.567528714439536	0.0
31	5.67310867066583E-5	0.0	0.0	8.913871998783685	0.0
32	5.67310867066583E-5	0.0	0.0	9.20121495295291	0.0
33	5.67310867066583E-5	0.0	0.0	9.467056825260311	0.0
34	5.67310867066583E-5	0.0	0.0	9.768185433499253	0.0
35	5.67310867066583E-5	0.0	0.0	10.186350273614032	0.0
36	1.134621734133166E-4	0.0	0.0	10.525715634293261	0.0
37	1.134621734133166E-4	0.0	0.0	10.847664551353548	0.0
38	1.134621734133166E-4	0.0	0.0	11.162749006922327	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTTTGCG	20	7.8576274E-4	44.0	1
AACCGCA	20	7.8576274E-4	44.0	43
CGGCGAA	20	7.8576274E-4	44.0	31
ATCTCGA	25	4.4438104E-5	44.0	34
CCTAGCG	20	7.8576274E-4	44.0	1
CCGGATG	20	7.8576274E-4	44.0	1
CCGGATC	25	4.4438104E-5	44.0	39
GATCCGG	145	0.0	44.0	2
GCCGGAT	20	7.8576274E-4	44.0	19
CGTCTGG	50	2.7284841E-11	44.0	2
GTTAGCG	160	0.0	44.0	1
TTCCGTA	20	7.8576274E-4	44.0	42
CACTGCG	55	1.8189894E-12	44.0	1
TACGGAC	30	2.5284899E-6	44.0	17
TCGATAG	30	2.5284899E-6	44.0	1
TGGCCGG	20	7.8576274E-4	44.0	11
ACGATGA	20	7.8576274E-4	44.0	30
GTAGCGC	20	7.8576274E-4	44.0	9
ACCTACG	20	7.8576274E-4	44.0	35
TACGATC	20	7.8576274E-4	44.0	18
>>END_MODULE