FastQCFastQC Report
Thu 26 May 2016
SRR1546156_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546156_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1813268
Sequences flagged as poor quality0
Sequence length50
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT243401.3423277750448361No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA39490.2177835819084658No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCA30530.16837003686162222No Hit
TGTGTTGGGTTAACAGAGAAGTTATAGGTGGATTATTTATAGTGTGATTA19800.10919511070619456No Hit
GAAGAAGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTAT19610.10814727883578158No Hit
AAGAATGGGATGCTACTACCACTAACCTGACTATCAAGCCCTAAAAAAAC18810.10373535517088484No Hit
TAGTATGGGGTCTATTTAAGAAGCAAAACTCAAAGCTTAAAAATTTCTCT18770.10351475898764001No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACGGGC600.044.0000044
TCCGATA302.528528E-644.00000416
GCCGGAG302.528528E-644.0000041
CCGTACT302.528528E-644.00000434
TTACGCG650.044.0000041
GATCTCG600.044.0000049
CACGTAA302.528528E-644.00000436
CGCGTGG302.528528E-644.0000043
CCGTTTA650.044.00000431
AATCGTC302.528528E-644.00000439
ACGCGAG207.8576873E-444.01
GTGTTCG207.8576873E-444.021
CGTGGAT454.802132E-1044.021
CTCCGTA700.044.032
CACGGTA254.443861E-544.04
CTTCCGT254.443861E-544.011
ACGTTAG1000.044.01
CGTGCCG207.8576873E-444.041
AGCTTCG207.8576873E-444.01
TAGAACG254.443861E-544.02
Produced by FastQC (version 0.11.5)