##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546156_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1813268
Sequences flagged as poor quality	0
Sequence length	50
%GC	37
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.802805762854693	31.0	31.0	34.0	30.0	34.0
2	32.19483882139871	33.0	31.0	34.0	30.0	34.0
3	32.23122064692037	33.0	31.0	34.0	30.0	34.0
4	35.93178614523612	37.0	35.0	37.0	35.0	37.0
5	35.834592018388896	37.0	35.0	37.0	35.0	37.0
6	35.911542033499735	37.0	35.0	37.0	35.0	37.0
7	36.14418607729249	37.0	35.0	37.0	35.0	37.0
8	36.05220298378397	37.0	35.0	37.0	35.0	37.0
9	37.78865120875679	39.0	38.0	39.0	35.0	39.0
10	37.45847497446599	39.0	37.0	39.0	35.0	39.0
11	37.27459426846997	39.0	37.0	39.0	34.0	39.0
12	36.80110386330096	39.0	35.0	39.0	33.0	39.0
13	36.54747726204841	39.0	35.0	39.0	33.0	39.0
14	37.71598241407227	40.0	35.0	41.0	33.0	41.0
15	37.918878511064	40.0	35.0	41.0	33.0	41.0
16	38.01716900094195	40.0	35.0	41.0	34.0	41.0
17	37.94776227231716	40.0	35.0	41.0	34.0	41.0
18	37.77212745165083	39.0	36.0	41.0	34.0	41.0
19	37.55133824674566	39.0	36.0	41.0	34.0	41.0
20	37.28598420090135	38.0	35.0	41.0	34.0	41.0
21	37.19397022392719	38.0	35.0	41.0	33.0	41.0
22	37.09477253224565	38.0	35.0	41.0	33.0	41.0
23	37.026049100298465	38.0	35.0	41.0	33.0	41.0
24	36.98360639464216	38.0	35.0	41.0	33.0	41.0
25	36.946829701952495	38.0	35.0	41.0	33.0	41.0
26	36.90465998407296	38.0	35.0	41.0	33.0	41.0
27	36.82918520593757	38.0	35.0	41.0	33.0	41.0
28	36.76921778799383	38.0	35.0	41.0	33.0	41.0
29	36.69206261843257	38.0	35.0	41.0	32.0	41.0
30	36.51584487235202	38.0	35.0	41.0	32.0	41.0
31	36.23618185508154	38.0	35.0	41.0	31.0	41.0
32	35.87008208383978	38.0	35.0	41.0	30.0	41.0
33	35.38929656289087	38.0	35.0	41.0	26.0	41.0
34	34.9439542307039	38.0	35.0	41.0	23.0	41.0
35	34.64594257440158	38.0	35.0	41.0	21.0	41.0
36	34.441434470800786	38.0	34.0	41.0	18.0	41.0
37	34.342658117829245	38.0	34.0	40.0	18.0	41.0
38	34.33988026039174	38.0	34.0	41.0	18.0	41.0
39	34.27666290917835	38.0	34.0	41.0	18.0	41.0
40	34.15404286625033	38.0	34.0	40.0	18.0	41.0
41	34.08200607963081	38.0	34.0	40.0	17.0	41.0
42	34.01364497691461	38.0	34.0	40.0	15.0	41.0
43	33.915144920662584	38.0	34.0	40.0	15.0	41.0
44	33.79087040636023	38.0	34.0	40.0	15.0	41.0
45	33.73151790027729	38.0	34.0	40.0	15.0	41.0
46	33.67311120033001	37.0	34.0	40.0	15.0	41.0
47	33.608039186705994	37.0	33.0	40.0	15.0	41.0
48	33.56101028640003	37.0	33.0	40.0	13.0	41.0
49	33.45526971192344	37.0	33.0	40.0	12.0	41.0
50	33.29018710968263	37.0	33.0	40.0	11.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	1.0
11	2.0
12	4.0
13	14.0
14	13.0
15	30.0
16	98.0
17	271.0
18	660.0
19	1350.0
20	2592.0
21	4352.0
22	6927.0
23	10654.0
24	16989.0
25	28662.0
26	45253.0
27	53875.0
28	48222.0
29	40670.0
30	39439.0
31	42363.0
32	50465.0
33	65515.0
34	117294.0
35	236584.0
36	100684.0
37	124520.0
38	215301.0
39	560463.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	25.187782500987165	23.716957449202212	31.04124707434312	20.054012975467497
2	33.41739886216489	26.380104871425512	29.50904113457029	10.69345513183931
3	23.348120630816847	24.28532351533254	41.90969012854139	10.456865725309221
4	21.643077581471683	26.40017912410079	40.36347633113252	11.593266963295001
5	20.12708546116735	30.396775324993325	39.08048892938054	10.395650284458778
6	17.471989799632485	37.97353728185795	35.344637417083405	9.209835501426154
7	78.18458165036829	5.118382941738342	15.153303317546	1.5437320903473728
8	79.90969895238874	3.6996185891991695	14.70284591136004	1.6878365470520629
9	75.11735716948625	5.94048976764604	16.328088291416382	2.614064771451324
10	37.837980927253994	33.33417895203577	22.215634975083663	6.612205145626571
11	33.6560839324358	20.406856570567616	35.59264267609642	10.344416820900165
12	33.46085631026412	20.663961422139472	33.721876744088576	12.153305523507832
13	17.01110922378821	37.775331611212465	32.35644151884884	12.857117646150487
14	11.641191484104942	38.588007950286446	34.37230459038598	15.398495975222637
15	10.501812197645357	26.646419613647844	50.143332370063334	12.708435818643466
16	11.447177140941108	23.176110756931685	46.762144371378085	18.614567730749123
17	12.199189529622759	25.696532448595576	35.25573715523574	26.848540866545928
18	14.68321285105125	28.483710074848283	40.75784715772848	16.075229916371985
19	18.20387278659305	28.222469044840587	33.78662172387094	19.78703644469543
20	20.699091364321216	24.824681183366167	39.23192820917812	15.244299243134495
21	16.116701998822016	29.788812243970554	37.69597213429014	16.398513622917296
22	19.486474145024342	26.332511244890437	32.02174195982061	22.159272650264604
23	14.061076465254999	30.104044189827427	33.683603306295595	22.15127603862198
24	12.658360485046888	26.488307299307106	48.39615544971841	12.457176765927596
25	11.398370235398188	32.245371340585066	41.226834643307	15.129423780709747
26	13.07208862672258	32.1299443877022	35.97818965536258	18.819777330212634
27	12.622623903361225	36.33516942889854	34.14944729626288	16.892759371477357
28	10.286069130431905	31.937584515912704	41.84737170677473	15.92897464688066
29	12.11216433533267	24.789606390230237	39.49394132582718	23.604287948609915
30	13.262297685725441	35.2683111376807	35.332504627004944	16.13688654958892
31	18.281026301682928	34.5431563343091	31.80230390653781	15.373513457470159
32	20.19806228312638	28.440528371978107	33.03929700408324	18.32211234081228
33	19.912996865328235	33.41298693849999	29.430233148106073	17.2437830480657
34	15.431254508434494	28.212707663732	34.53543546789554	21.82060235993797
35	18.22686993869632	27.991835735258107	33.75678609008707	20.024508235958503
36	23.800894296926874	30.999995588076334	30.242523443859376	14.956586671137417
37	15.135655622886413	33.68376875343303	36.307484607901316	14.873091015779243
38	15.347427958801457	31.410966277461466	35.617625193848895	17.62398056988818
39	16.581884200239568	32.27399369536108	34.99874260175551	16.145379502643845
40	18.940167697218502	28.59345667601259	32.25077594707456	20.215599679694343
41	14.50392330311901	29.271459045215597	33.86526426319772	22.359353388467675
42	16.392171482649008	28.36640805440784	31.70011272464964	23.54130773829351
43	17.5158332910524	30.26386612458831	30.90122364702846	21.31907693733083
44	16.10010213603284	31.462861529569814	30.34090934158657	22.09612699281077
45	15.357354787047475	39.02192064272904	26.440437927542977	19.180286642680507
46	18.822093590136703	35.91145930993102	28.99549321997631	16.270953879955968
47	16.477983397931247	30.82511796380899	33.13216799722931	19.564730641030447
48	16.99279974057889	28.138146153795248	35.76597612708105	19.103077978544817
49	18.487945521566587	30.52747856356589	32.880081708826275	18.104494206041245
50	16.9116754941906	32.30195426158737	32.04749656421445	18.73887368000759
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	4722.0
1	5570.5
2	6419.0
3	21739.5
4	37060.0
5	28066.5
6	19073.0
7	20753.5
8	22434.0
9	24434.0
10	26434.0
11	26730.0
12	27026.0
13	26120.0
14	25214.0
15	23021.0
16	20828.0
17	19634.0
18	18440.0
19	16766.5
20	15093.0
21	15043.0
22	14993.0
23	15753.0
24	16513.0
25	22277.5
26	28042.0
27	31450.5
28	34859.0
29	42796.5
30	50734.0
31	66089.0
32	81444.0
33	104959.5
34	128475.0
35	129428.0
36	130381.0
37	126675.0
38	122969.0
39	133485.5
40	144002.0
41	151754.0
42	159506.0
43	163296.5
44	167087.0
45	146196.0
46	125305.0
47	117077.0
48	108849.0
49	102419.0
50	95989.0
51	85221.0
52	74453.0
53	59006.0
54	43559.0
55	33578.0
56	23597.0
57	18901.5
58	14206.0
59	11751.0
60	9296.0
61	7845.5
62	6395.0
63	5137.0
64	3879.0
65	3265.0
66	2651.0
67	2016.0
68	1381.0
69	1063.0
70	745.0
71	664.5
72	584.0
73	417.5
74	251.0
75	222.0
76	193.0
77	135.5
78	78.0
79	68.5
80	59.0
81	42.0
82	25.0
83	20.5
84	16.0
85	11.0
86	6.0
87	3.5
88	1.0
89	1.0
90	1.0
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1813268.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	22.43172857009789
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.2080333421458	17.5434137593218
2	9.06811004438987	4.068267663190637
3	3.42918568976988	2.3076768782814554
4	1.7401064988498112	1.5613438666104924
5	1.0968821118055443	1.2302480902708868
6	0.7473418520401854	1.0058501744423818
7	0.5579343256555076	0.8760801947131464
8	0.4173574829405921	0.7489639819218098
9	0.339799294485675	0.6860056987992078
>10	3.0042180347492455	14.545567346739263
>50	0.7008356977884475	11.090586657025835
>100	0.6039211722493076	27.331105635911733
>500	0.06847571208327789	10.597987275713013
>1k	0.017551536310154263	5.057193903053863
>5k	0.0	0.0
>10k+	2.4720473676273605E-4	1.349708874004441
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	24340	1.3423277750448361	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3949	0.2177835819084658	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCA	3053	0.16837003686162222	No Hit
TGTGTTGGGTTAACAGAGAAGTTATAGGTGGATTATTTATAGTGTGATTA	1980	0.10919511070619456	No Hit
GAAGAAGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTAT	1961	0.10814727883578158	No Hit
AAGAATGGGATGCTACTACCACTAACCTGACTATCAAGCCCTAAAAAAAC	1881	0.10373535517088484	No Hit
TAGTATGGGGTCTATTTAAGAAGCAAAACTCAAAGCTTAAAAATTTCTCT	1877	0.10351475898764001	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.06518617214884947	0.0
2	0.0	0.0	0.0	0.27673791188064867	0.0
3	0.0	0.0	0.0	0.3975694712530084	0.0
4	0.0	0.0	0.0	0.6408319123262529	0.0
5	0.0	0.0	0.0	1.169766410701562	0.0
6	0.0	0.0	0.0	1.5971163666926236	0.0
7	0.0	0.0	0.0	1.898064709684393	0.0
8	0.0	0.0	0.0	2.6249842825219436	0.0
9	0.0	0.0	0.0	2.9006192134863684	0.0
10	0.0	0.0	0.0	3.447366853658698	0.0
11	0.0	0.0	0.0	3.9618523020314704	0.0
12	0.0	0.0	0.0	4.438064312611263	0.0
13	0.0	0.0	0.0	4.598106843555393	0.0
14	0.0	0.0	0.0	4.64994694661793	0.0
15	0.0	0.0	0.0	4.745520243008755	0.0
16	0.0	0.0	0.0	4.962642036367487	0.0
17	0.0	0.0	0.0	5.242027102447073	0.0
18	0.0	0.0	0.0	5.640589256524684	0.0
19	0.0	0.0	0.0	5.8396773119031495	0.0
20	0.0	0.0	0.0	6.037882982548636	0.0
21	0.0	0.0	0.0	6.34131303260191	0.0
22	0.0	0.0	0.0	6.639448774257308	0.0
23	0.0	0.0	0.0	7.001116216687219	0.0
24	0.0	0.0	0.0	7.268809685054829	0.0
25	0.0	0.0	0.0	7.486207223642617	0.0
26	0.0	0.0	0.0	7.7321168189147995	0.0
27	0.0	0.0	0.0	7.9597720800234715	0.0
28	0.0	0.0	0.0	8.20921121422757	0.0
29	0.0	0.0	0.0	8.478504004923707	0.0
30	0.0	0.0	0.0	8.866146647930698	0.0
31	5.5149045811209376E-5	0.0	0.0	9.244027909829104	0.0
32	5.5149045811209376E-5	0.0	0.0	9.541115819614088	0.0
33	5.5149045811209376E-5	0.0	0.0	9.82116267424341	0.0
34	5.5149045811209376E-5	0.0	0.0	10.129114946053203	0.0
35	5.5149045811209376E-5	0.0	0.0	10.565564494603114	0.0
36	5.5149045811209376E-5	0.0	0.0	10.89960226508161	0.0
37	5.5149045811209376E-5	0.0	0.0	11.234467271247272	0.0
38	5.5149045811209376E-5	0.0	0.0	11.558412766342316	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CACGGGC	60	0.0	44.000004	4
TCCGATA	30	2.528528E-6	44.000004	16
GCCGGAG	30	2.528528E-6	44.000004	1
CCGTACT	30	2.528528E-6	44.000004	34
TTACGCG	65	0.0	44.000004	1
GATCTCG	60	0.0	44.000004	9
CACGTAA	30	2.528528E-6	44.000004	36
CGCGTGG	30	2.528528E-6	44.000004	3
CCGTTTA	65	0.0	44.000004	31
AATCGTC	30	2.528528E-6	44.000004	39
ACGCGAG	20	7.8576873E-4	44.0	1
GTGTTCG	20	7.8576873E-4	44.0	21
CGTGGAT	45	4.802132E-10	44.0	21
CTCCGTA	70	0.0	44.0	32
CACGGTA	25	4.443861E-5	44.0	4
CTTCCGT	25	4.443861E-5	44.0	11
ACGTTAG	100	0.0	44.0	1
CGTGCCG	20	7.8576873E-4	44.0	41
AGCTTCG	20	7.8576873E-4	44.0	1
TAGAACG	25	4.443861E-5	44.0	2
>>END_MODULE