FastQCFastQC Report
Thu 26 May 2016
SRR1546155_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546155_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1629308
Sequences flagged as poor quality0
Sequence length50
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT246451.5126053514743683No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA32430.19904155629261014No Hit
GATAATCGGGGGGAATTCAATACCTTTACAAATGCTTTAACAAGAGGAAA16460.10102448401407224No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACCGGC207.85745E-444.00000438
TCGTTGC207.85745E-444.00000427
CGTGCCA207.85745E-444.00000418
GCACGTC207.85745E-444.00000425
GTTAGCG600.044.0000041
CGTTAGT408.3164196E-944.00000432
GTCCGTT207.85745E-444.00000410
TCGAGTA302.5283698E-644.00000410
ATACGAG408.3164196E-944.0000041
CAATACG207.85745E-444.0000041
ACCCACG207.85745E-444.00000438
AACGTAC1150.044.038
TTGGCGA1050.044.01
ACTTCGC254.443661E-544.031
TGTATCG254.443661E-544.01
ATGTACG351.4465877E-744.01
ACTACGT254.443661E-544.022
ATGCGTA254.443661E-544.044
TTACGCA750.044.014
TAGTGCG2000.044.01
Produced by FastQC (version 0.11.5)