##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546155_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1629308
Sequences flagged as poor quality	0
Sequence length	50
%GC	38
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.860254783012174	31.0	31.0	34.0	30.0	34.0
2	32.235103491789154	33.0	31.0	34.0	30.0	34.0
3	32.135697486294795	33.0	31.0	34.0	30.0	34.0
4	35.89487131960317	37.0	35.0	37.0	35.0	37.0
5	35.88334311253612	37.0	35.0	37.0	35.0	37.0
6	35.920084477581895	37.0	35.0	37.0	35.0	37.0
7	36.13391943082585	37.0	35.0	37.0	35.0	37.0
8	36.01919342444768	37.0	35.0	37.0	35.0	37.0
9	37.751982436715465	39.0	38.0	39.0	35.0	39.0
10	37.47786360835398	39.0	37.0	39.0	35.0	39.0
11	37.3740538928183	39.0	37.0	39.0	34.0	39.0
12	36.92977263967279	39.0	35.0	39.0	33.0	39.0
13	36.78143665899879	39.0	35.0	39.0	33.0	39.0
14	37.96915561698586	40.0	36.0	41.0	33.0	41.0
15	38.10706938160249	40.0	36.0	41.0	33.0	41.0
16	38.178774669982595	40.0	36.0	41.0	34.0	41.0
17	38.065873364643146	40.0	36.0	41.0	34.0	41.0
18	37.86826370459115	39.0	36.0	41.0	34.0	41.0
19	37.66916629636631	39.0	36.0	41.0	34.0	41.0
20	37.3480041833711	39.0	35.0	41.0	33.0	41.0
21	37.27429743179313	39.0	35.0	41.0	33.0	41.0
22	37.20687739825742	39.0	35.0	41.0	33.0	41.0
23	37.137333763781925	39.0	35.0	41.0	33.0	41.0
24	37.06945341212343	38.0	35.0	41.0	33.0	41.0
25	37.0508933854127	38.0	35.0	41.0	33.0	41.0
26	36.975152641489515	38.0	35.0	41.0	33.0	41.0
27	36.9200421283146	38.0	35.0	41.0	33.0	41.0
28	36.85306522769176	38.0	35.0	41.0	33.0	41.0
29	36.78046078457848	38.0	35.0	41.0	32.0	41.0
30	36.607844557321265	38.0	35.0	41.0	32.0	41.0
31	36.33402462886084	38.0	35.0	41.0	31.0	41.0
32	36.01695136831096	38.0	35.0	41.0	30.0	41.0
33	35.62345486550118	38.0	35.0	41.0	27.0	41.0
34	35.13711158356799	38.0	35.0	41.0	23.0	41.0
35	34.88407593898759	38.0	35.0	41.0	22.0	41.0
36	34.707152975373596	38.0	35.0	41.0	21.0	41.0
37	34.65088184677176	38.0	34.0	41.0	21.0	41.0
38	34.57697930655223	38.0	34.0	41.0	20.0	41.0
39	34.520375521386995	38.0	34.0	41.0	19.0	41.0
40	34.388842993467165	38.0	34.0	40.0	18.0	41.0
41	34.265338413608724	38.0	34.0	40.0	18.0	41.0
42	34.176265629334665	38.0	34.0	40.0	18.0	41.0
43	34.03455945714377	38.0	34.0	40.0	17.0	41.0
44	33.88664512787024	38.0	34.0	40.0	15.0	41.0
45	33.8239491857893	37.0	34.0	40.0	17.0	41.0
46	33.76042773987484	37.0	34.0	40.0	15.0	41.0
47	33.714117281692594	37.0	33.0	40.0	15.0	41.0
48	33.65277651616515	37.0	33.0	40.0	15.0	41.0
49	33.5622656980755	37.0	33.0	40.0	15.0	41.0
50	33.39644867637058	37.0	33.0	40.0	15.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	2.0
11	4.0
12	0.0
13	7.0
14	16.0
15	35.0
16	109.0
17	229.0
18	559.0
19	1198.0
20	2185.0
21	3734.0
22	5852.0
23	8935.0
24	14048.0
25	23254.0
26	36797.0
27	44571.0
28	42717.0
29	37675.0
30	37088.0
31	39443.0
32	45847.0
33	57590.0
34	101101.0
35	196384.0
36	99366.0
37	119662.0
38	200747.0
39	510152.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	25.988579200494932	23.80483002599877	29.346139588095067	20.86045118541123
2	35.56718557817184	25.00552381747343	28.819474279878328	10.607816324476403
3	24.27042646325925	24.24219361839505	40.18251920447209	11.30486071387362
4	22.369926373650653	26.056460779668424	39.20633790541751	12.367274941263409
5	20.306903298823794	29.07989158587572	37.90455825417908	12.708646861121409
6	18.68578562187137	36.769904769386756	35.395333478998445	9.148976129743424
7	75.73700000245503	5.307590707220489	16.6957383134435	2.2596709768809826
8	75.93720769799202	4.724152830526824	16.422125221259577	2.9165142502215664
9	70.56658409582474	6.290707465991698	19.006535289828566	4.136173148355008
10	36.28178343198462	28.75527524568713	24.30430587709629	10.658635445231964
11	31.32421862533051	21.61169036179777	33.6853437164735	13.378747296398224
12	29.22989391815421	20.37944943497485	37.352360634085144	13.038296012785796
13	16.51124280983092	34.22330216263592	34.89604175515004	14.369413272383122
14	11.54048221699028	35.62678143113518	38.00147056296292	14.831265788911614
15	10.912546921760649	24.47112516479389	51.087701036268165	13.528626877177304
16	13.769158440270349	21.932992411502305	45.5944486861907	18.703400462036644
17	13.06517859115649	22.731061284913594	37.61719699406128	26.58656312986863
18	15.919150952428884	25.40845561428533	42.39922715655972	16.27316627672607
19	20.762188610133872	25.96335376736627	35.1175468358346	18.156910786665257
20	22.66066330000221	23.45916180366143	37.651015032148614	16.229159864187743
21	16.98334507656011	28.004772578297043	37.489228555926815	17.522653789216037
22	17.77466261750387	25.013379913435642	34.46113319274195	22.750824276318536
23	14.416918102654625	28.812538820161688	35.02014352105311	21.750399556130578
24	14.080149364024482	23.82091047242142	46.821472674288714	15.277467489265382
25	13.387033022608371	28.44575733992591	41.56212330633618	16.605086331129534
26	14.571278113162153	30.488587793099892	36.61591301337746	18.3242210803605
27	13.342719731321518	32.10301551333449	35.68521114485414	18.869053610489853
28	12.327994461452347	28.52419554804862	41.72691719429353	17.420892796205507
29	13.81101670156901	24.960903647438055	39.90675796104849	21.32132168994444
30	14.477434591863538	31.14481730894343	37.86975820409646	16.50798989509657
31	18.111185853135193	31.812708217230874	34.137621616048044	15.93848431358589
32	19.72604320361773	27.155209450883444	34.94581748816062	18.172929857338207
33	19.645702347254172	29.010721115958432	33.21809013397098	18.12548640281641
34	15.176136126502785	27.343939881225648	36.40502593739183	21.07489805487974
35	16.997522874741914	27.55065340623136	36.085565160178426	19.366258558848294
36	20.545961843923923	29.363508925261524	33.520672580015564	16.56985665079899
37	16.64405993219207	31.957923241032386	35.83601136187878	15.562005464896753
38	15.6023907082025	29.181959457634775	36.62941567831251	18.586234155850214
39	16.891465579252053	31.203983531658842	34.35194573401714	17.55260515507197
40	18.306728991694634	27.375241513575087	34.04009554976714	20.277933944963138
41	15.037120053421452	28.768225528874837	33.332371779921296	22.86228263778242
42	17.274818511908123	28.490929891708628	31.926191978435014	22.308059617948235
43	17.087499723809128	28.211486103302754	33.80079150166819	20.90022267121993
44	15.813646038686363	28.119422478745577	34.97932864749943	21.08760283506863
45	15.817267207918945	35.29676402497257	29.371549148472848	19.514419618635642
46	19.387003562248513	31.74623828030059	31.65442015874224	17.212337998708655
47	17.023177938118515	29.924483277563237	33.190593798103244	19.861744986215008
48	17.290898958330775	26.444846523800287	35.41847213663715	20.845782381231786
49	17.75631126834214	26.63725949912478	36.446577319941966	19.15985191259111
50	16.91933016961802	30.21227416792896	34.35077959477275	18.51761606768027
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	4485.0
1	6410.0
2	8335.0
3	25703.5
4	43072.0
5	31144.5
6	19217.0
7	20531.0
8	21845.0
9	23158.5
10	24472.0
11	24441.5
12	24411.0
13	23173.0
14	21935.0
15	20083.5
16	18232.0
17	17178.0
18	16124.0
19	15549.0
20	14974.0
21	16676.0
22	18378.0
23	22826.0
24	27274.0
25	24649.5
26	22025.0
27	25148.0
28	28271.0
29	37223.5
30	46176.0
31	55977.0
32	65778.0
33	79150.0
34	92522.0
35	100556.5
36	108591.0
37	101219.5
38	93848.0
39	100273.0
40	106698.0
41	117259.0
42	127820.0
43	125623.0
44	123426.0
45	121462.5
46	119499.0
47	115867.0
48	112235.0
49	99977.5
50	87720.0
51	72559.0
52	57398.0
53	49233.5
54	41069.0
55	35380.5
56	29692.0
57	27867.5
58	26043.0
59	24248.5
60	22454.0
61	20744.0
62	19034.0
63	16216.5
64	13399.0
65	10753.5
66	8108.0
67	6620.5
68	5133.0
69	4556.5
70	3980.0
71	3187.0
72	2394.0
73	1893.0
74	1392.0
75	1110.5
76	829.0
77	646.0
78	463.0
79	389.0
80	315.0
81	242.0
82	169.0
83	104.5
84	40.0
85	27.0
86	14.0
87	11.0
88	8.0
89	7.0
90	6.0
91	5.0
92	4.0
93	2.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1629308.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	23.336348658280507
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.10826308691021	18.22761660488838
2	8.42406555547595	3.931738618455964
3	3.1986634784655665	2.239353785219423
4	1.7280293948892629	1.613035858035733
5	1.07328895579275	1.2523322641730712
6	0.7488398303382794	1.0485112421989022
7	0.5688357937259623	0.929218528818915
8	0.45731086913662083	0.8537572709914778
9	0.3887054819864069	0.8163869987717789
>10	4.048660906338426	21.182965717846898
>50	0.7129133599163143	11.467257847159344
>100	0.4735945634471479	23.13981830817307
>500	0.06194585121941027	9.71393223752606
>1k	0.006618146497800243	2.0615719354270334
>5k	0.0	0.0
>10k+	2.647258599120097E-4	1.5225027823139623
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	24645	1.5126053514743683	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3243	0.19904155629261014	No Hit
GATAATCGGGGGGAATTCAATACCTTTACAAATGCTTTAACAAGAGGAAA	1646	0.10102448401407224	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.051617005501722206	0.0
2	0.0	0.0	0.0	0.288588775111888	0.0
3	0.0	0.0	0.0	0.4882440889015459	0.0
4	0.0	0.0	0.0	0.8247059487831644	0.0
5	0.0	0.0	0.0	1.487564045594817	0.0
6	0.0	0.0	0.0	2.1348940777311594	0.0
7	0.0	0.0	0.0	2.5491190124887377	0.0
8	0.0	0.0	0.0	3.4680367370687435	0.0
9	0.0	0.0	0.0	3.908837371448492	0.0
10	0.0	0.0	0.0	4.637183393195148	0.0
11	0.0	0.0	0.0	5.278375850361012	0.0
12	6.137574970478264E-5	0.0	0.0	5.852423237349845	0.0
13	6.137574970478264E-5	0.0	0.0	6.074296572532633	0.0
14	6.137574970478264E-5	0.0	0.0	6.160897755366082	0.0
15	6.137574970478264E-5	0.0	0.0	6.291996356735497	0.0
16	6.137574970478264E-5	0.0	0.0	6.560944891941855	0.0
17	6.137574970478264E-5	0.0	0.0	6.868682900961635	0.0
18	6.137574970478264E-5	0.0	0.0	7.317707885801825	0.0
19	6.137574970478264E-5	0.0	0.0	7.549769595435608	0.0
20	6.137574970478264E-5	0.0	0.0	7.829029256592369	0.0
21	6.137574970478264E-5	0.0	0.0	8.131734454136357	0.0
22	6.137574970478264E-5	0.0	0.0	8.432168748941269	0.0
23	6.137574970478264E-5	0.0	0.0	8.765070815340009	0.0
24	6.137574970478264E-5	0.0	0.0	9.015361122636113	0.0
25	6.137574970478264E-5	0.0	0.0	9.228580477110528	0.0
26	6.137574970478264E-5	0.0	0.0	9.468436906956818	0.0
27	6.137574970478264E-5	0.0	0.0	9.693501781124256	0.0
28	6.137574970478264E-5	0.0	0.0	9.931885192977632	0.0
29	6.137574970478264E-5	0.0	0.0	10.163640023862891	0.0
30	6.137574970478264E-5	0.0	0.0	10.490036260792925	0.0
31	6.137574970478264E-5	0.0	0.0	10.79090018584577	0.0
32	6.137574970478264E-5	0.0	0.0	11.050458231347296	0.0
33	6.137574970478264E-5	0.0	0.0	11.298784514652846	0.0
34	6.137574970478264E-5	0.0	0.0	11.559385947899354	0.0
35	6.137574970478264E-5	0.0	0.0	11.893699656541305	0.0
36	6.137574970478264E-5	0.0	0.0	12.169338148465483	0.0
37	6.137574970478264E-5	0.0	0.0	12.444362882892614	0.0
38	6.137574970478264E-5	0.0	0.0	12.690234136209973	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AACCGGC	20	7.85745E-4	44.000004	38
TCGTTGC	20	7.85745E-4	44.000004	27
CGTGCCA	20	7.85745E-4	44.000004	18
GCACGTC	20	7.85745E-4	44.000004	25
GTTAGCG	60	0.0	44.000004	1
CGTTAGT	40	8.3164196E-9	44.000004	32
GTCCGTT	20	7.85745E-4	44.000004	10
TCGAGTA	30	2.5283698E-6	44.000004	10
ATACGAG	40	8.3164196E-9	44.000004	1
CAATACG	20	7.85745E-4	44.000004	1
ACCCACG	20	7.85745E-4	44.000004	38
AACGTAC	115	0.0	44.0	38
TTGGCGA	105	0.0	44.0	1
ACTTCGC	25	4.443661E-5	44.0	31
TGTATCG	25	4.443661E-5	44.0	1
ATGTACG	35	1.4465877E-7	44.0	1
ACTACGT	25	4.443661E-5	44.0	22
ATGCGTA	25	4.443661E-5	44.0	44
TTACGCA	75	0.0	44.0	14
TAGTGCG	200	0.0	44.0	1
>>END_MODULE