FastQCFastQC Report
Thu 26 May 2016
SRR1546154_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546154_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1663320
Sequences flagged as poor quality0
Sequence length50
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT249211.4982685231945747No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA32690.196534641560253No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGATCA650.044.00000417
GCCCAAT351.4466059E-744.09
ACCGCTC207.8575E-444.018
CGACCGG302.5284025E-644.033
GTTAGCG351.4466059E-744.01
CCTCGCA408.3164196E-944.037
CGTTAGT302.5284025E-644.032
CGTTAGA207.8575E-444.042
TATTGCG700.044.01
AGTGCCG207.8575E-444.01
CTACGGT351.4466059E-744.03
ACTACGT302.5284025E-644.022
TCCGTAG900.044.034
CGTAATA207.8575E-444.023
ACGTAAG302.5284025E-644.01
TTCGATC254.4437013E-544.027
GTAACGT254.4437013E-544.031
CCGCAAT207.8575E-444.033
TACGTTA454.802132E-1044.027
TACGTAC502.7284841E-1144.024
Produced by FastQC (version 0.11.5)