##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546154_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1663320
Sequences flagged as poor quality	0
Sequence length	50
%GC	38
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.797969723204194	31.0	31.0	34.0	30.0	34.0
2	32.2101531876007	33.0	31.0	34.0	30.0	34.0
3	32.23344335425534	33.0	31.0	34.0	30.0	34.0
4	35.938314936392274	37.0	35.0	37.0	35.0	37.0
5	35.85313048601592	37.0	35.0	37.0	35.0	37.0
6	35.91377485991872	37.0	35.0	37.0	35.0	37.0
7	36.11366784503283	37.0	35.0	37.0	35.0	37.0
8	36.003060745977926	37.0	35.0	37.0	35.0	37.0
9	37.728869369694344	39.0	38.0	39.0	35.0	39.0
10	37.485292667676696	39.0	37.0	39.0	35.0	39.0
11	37.36917249837674	39.0	37.0	39.0	34.0	39.0
12	36.91555082605873	39.0	35.0	39.0	33.0	39.0
13	36.73527583387442	39.0	35.0	39.0	33.0	39.0
14	37.91566265060241	40.0	36.0	41.0	33.0	41.0
15	38.08090505735517	40.0	36.0	41.0	33.0	41.0
16	38.143946444460475	40.0	36.0	41.0	34.0	41.0
17	38.041850034870016	40.0	36.0	41.0	34.0	41.0
18	37.853367361662215	39.0	36.0	41.0	33.0	41.0
19	37.658155375995	39.0	36.0	41.0	34.0	41.0
20	37.335574032657576	39.0	35.0	41.0	33.0	41.0
21	37.26080970588943	39.0	35.0	41.0	33.0	41.0
22	37.20146574321237	39.0	35.0	41.0	33.0	41.0
23	37.12145047254888	39.0	35.0	41.0	33.0	41.0
24	37.054677392203544	38.0	35.0	41.0	33.0	41.0
25	37.02924151696607	38.0	35.0	41.0	33.0	41.0
26	36.9751172354087	38.0	35.0	41.0	33.0	41.0
27	36.90920328018662	38.0	35.0	41.0	33.0	41.0
28	36.83457121900777	38.0	35.0	41.0	33.0	41.0
29	36.7734013899911	38.0	35.0	41.0	32.0	41.0
30	36.59677933290046	38.0	35.0	41.0	32.0	41.0
31	36.327389197508595	38.0	35.0	41.0	31.0	41.0
32	36.01481975807421	38.0	35.0	41.0	30.0	41.0
33	35.60485955799245	38.0	35.0	41.0	27.0	41.0
34	35.19458973619027	38.0	35.0	41.0	24.0	41.0
35	34.90662770843854	38.0	35.0	41.0	22.0	41.0
36	34.72577014645408	38.0	35.0	41.0	21.0	41.0
37	34.60062585672029	38.0	34.0	41.0	21.0	41.0
38	34.53978067960465	38.0	34.0	41.0	20.0	41.0
39	34.48807745953875	38.0	34.0	41.0	19.0	41.0
40	34.3767982108073	38.0	34.0	41.0	18.0	41.0
41	34.27113784479234	38.0	34.0	40.0	18.0	41.0
42	34.16247805593632	38.0	34.0	40.0	18.0	41.0
43	34.05249500998004	38.0	34.0	40.0	17.0	41.0
44	33.89807733929731	38.0	34.0	40.0	15.0	41.0
45	33.83576942500541	38.0	34.0	40.0	17.0	41.0
46	33.78357141139408	37.0	34.0	40.0	16.0	41.0
47	33.720287737777454	37.0	33.0	40.0	15.0	41.0
48	33.676759733544955	37.0	33.0	40.0	15.0	41.0
49	33.57995875718443	37.0	33.0	40.0	15.0	41.0
50	33.38392432003463	37.0	33.0	40.0	14.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	2.0
11	4.0
12	3.0
13	4.0
14	11.0
15	31.0
16	93.0
17	224.0
18	577.0
19	1231.0
20	2195.0
21	3864.0
22	6158.0
23	9172.0
24	14329.0
25	23395.0
26	37075.0
27	45320.0
28	43868.0
29	39051.0
30	37870.0
31	40153.0
32	47508.0
33	60225.0
34	104817.0
35	197275.0
36	102153.0
37	122708.0
38	206576.0
39	517426.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	26.058906283817905	23.876884784647572	29.47875333670009	20.58545559483443
2	35.5779405045331	24.91565062645793	28.853437702907435	10.652971166101533
3	24.22804992424789	24.215725176153715	40.15174470336436	11.404480196234038
4	22.426652718659067	26.080369381718494	39.135343770290746	12.357634129331698
5	20.32825914436188	29.092116970877523	37.81437125748503	12.765252627275569
6	18.749248490969865	36.77831084818315	35.2790202727076	9.193420388139383
7	75.82623908808888	5.275172546473319	16.651696606786427	2.246891758651372
8	76.00058918307963	4.722302383185436	16.338106918692734	2.9390015150422046
9	70.59766010148378	6.2806315080682005	18.960332347353486	4.161376043094534
10	36.34946973522834	28.740230382608278	24.230334511699493	10.679965370463892
11	31.37466031791838	21.64508332732126	33.616141211552794	13.364115143207561
12	29.19883125315634	20.37659620518	37.323665921169706	13.100906620493951
13	16.59500276555323	34.12963230166174	34.84416708751172	14.431197845273308
14	11.536144578313252	35.61383257581223	38.01421253877786	14.83581030709665
15	10.970408580429503	24.395666498328644	51.04561960416516	13.58830531707669
16	13.885061202895413	21.911478248322634	45.49305004448934	18.710410504292618
17	13.070846259288652	22.701344299353103	37.623367722386554	26.604441718971692
18	16.01622057090638	25.319541639612343	42.339718154053344	16.32451963542794
19	20.785537359016907	25.932712887478054	35.162746795565496	18.119002957939543
20	22.72960103888608	23.368804559555585	37.55801649712623	16.3435779044321
21	16.975747300579563	27.991246422817017	37.40284491258447	17.63016136401895
22	17.760683452372366	25.006793641632395	34.42500541086502	22.807517495130224
23	14.47346271312796	28.7307313084674	34.99855710266215	21.797248875742493
24	14.119471899574346	23.765300723853496	46.74746891758652	15.367758458985643
25	13.42597936656807	28.380047134646368	41.498268523194575	16.695704975590985
26	14.56250150301806	30.41339008729529	36.63065435394272	18.393454055743934
27	13.30615876680374	32.05486617127191	35.74429454344323	18.89468051848111
28	12.300399201596807	28.527763749609214	41.676045499362715	17.495791549431257
29	13.817665873073132	24.959538753817668	39.861902700622856	21.360892672486354
30	14.491919774908016	31.02842507755573	37.93653656542337	16.543118582112886
31	18.107940745015995	31.757629319673907	34.12313926364139	16.01129067166871
32	19.69200153909049	27.09526729673184	34.98394776711637	18.2287833970613
33	19.680277998220426	28.992196330231103	33.1312675853113	18.19625808623716
34	15.165452228073972	27.39070052665753	36.21762499098189	21.226222254286608
35	17.00370343650049	27.550381165380085	36.02277372964914	19.42314166847029
36	20.484031936127746	29.320756078205036	33.53557944352259	16.659632542144625
37	16.608349565928386	31.84282038332973	35.90205131904865	15.646778731693239
38	15.60866219368492	29.151095399562323	36.629151335882455	18.61109107087031
39	16.905887021138447	31.071411394079313	34.4005362768439	17.62216530793834
40	18.315657840944617	27.347293365077075	34.01636486064017	20.32068393333814
41	15.051343096938652	28.757665392107352	33.30297236851598	22.888019142438015
42	17.40549022437054	28.39032777817858	31.866928793016374	22.337253204434504
43	17.08733136137364	28.09068609768415	33.81207464588895	21.009907895053267
44	15.837120938845201	28.030986220330423	35.043226799413226	21.08866604141115
45	15.895438039583484	35.22280739725369	29.325926460332347	19.555828102830482
46	19.383822716013754	31.616826587788278	31.635464011735564	17.3638866844624
47	17.045186734963806	29.961943582714092	33.075174951302216	19.91769473101989
48	17.314467450640887	26.36281653560349	35.47886155400043	20.84385445975519
49	17.84839958636943	26.534641560252986	36.47716614962845	19.13979270374913
50	16.941839213139986	30.007394848856507	34.33939350215232	18.71137243585119
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	4529.0
1	6474.5
2	8420.0
3	26113.0
4	43806.0
5	31480.0
6	19154.0
7	20674.5
8	22195.0
9	23492.0
10	24789.0
11	24656.5
12	24524.0
13	23484.0
14	22444.0
15	20643.0
16	18842.0
17	17570.0
18	16298.0
19	15646.0
20	14994.0
21	16795.5
22	18597.0
23	23084.0
24	27571.0
25	25105.0
26	22639.0
27	25821.5
28	29004.0
29	37671.0
30	46338.0
31	56731.5
32	67125.0
33	80489.0
34	93853.0
35	101947.5
36	110042.0
37	102755.0
38	95468.0
39	101798.5
40	108129.0
41	119304.5
42	130480.0
43	128269.0
44	126058.0
45	124389.5
46	122721.0
47	119170.0
48	115619.0
49	102995.5
50	90372.0
51	75067.0
52	59762.0
53	51222.0
54	42682.0
55	36913.0
56	31144.0
57	28775.5
58	26407.0
59	24901.5
60	23396.0
61	21381.0
62	19366.0
63	16532.0
64	13698.0
65	10907.5
66	8117.0
67	6660.5
68	5204.0
69	4576.0
70	3948.0
71	3164.5
72	2381.0
73	1902.0
74	1423.0
75	1122.0
76	821.0
77	631.0
78	441.0
79	347.0
80	253.0
81	216.5
82	180.0
83	111.0
84	42.0
85	25.0
86	8.0
87	11.5
88	15.0
89	15.0
90	15.0
91	9.0
92	3.0
93	2.5
94	2.0
95	1.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1663320.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	23.304872792144018
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.83238127778874	18.138737447885184
2	8.58206188833387	4.000077212038562
3	3.2856989055208023	2.297183850793474
4	1.6913802905273285	1.5766961005551594
5	1.0493094903748048	1.2227012096387146
6	0.7818592368382877	1.0932678033527459
7	0.5890835690190652	0.9609962347952054
8	0.48689959797532223	0.9077706554688758
9	0.3966457522491694	0.8319400919740051
>10	4.0573524161772	21.197249806311202
>50	0.6958540105553332	11.13863233444481
>100	0.48097859483970756	23.091747972661143
>500	0.06295122764458916	9.775538366107227
>1k	0.007283613115902878	2.2566815647455005
>5k	0.0	0.0
>10k+	2.6012903985367424E-4	1.5107793492282773
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	24921	1.4982685231945747	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3269	0.196534641560253	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	6.0120722410600484E-5	0.0	0.0	0.05284611499891783	0.0
2	6.0120722410600484E-5	0.0	0.0	0.2813048601591997	0.0
3	6.0120722410600484E-5	0.0	0.0	0.48872135247577136	0.0
4	6.0120722410600484E-5	0.0	0.0	0.8349565928384195	0.0
5	6.0120722410600484E-5	0.0	0.0	1.4940600726258326	0.0
6	6.0120722410600484E-5	0.0	0.0	2.137472043864079	0.0
7	6.0120722410600484E-5	0.0	0.0	2.556393237621143	0.0
8	6.0120722410600484E-5	0.0	0.0	3.482552966356444	0.0
9	6.0120722410600484E-5	0.0	0.0	3.9323160907101458	0.0
10	6.0120722410600484E-5	0.0	0.0	4.6746867710362405	0.0
11	6.0120722410600484E-5	0.0	0.0	5.350323449486569	0.0
12	6.0120722410600484E-5	0.0	0.0	5.943534617511964	0.0
13	6.0120722410600484E-5	0.0	0.0	6.176141692518577	0.0
14	6.0120722410600484E-5	0.0	0.0	6.2719741240410745	0.0
15	6.0120722410600484E-5	0.0	0.0	6.401955125892793	0.0
16	6.0120722410600484E-5	0.0	0.0	6.69402159536349	0.0
17	6.0120722410600484E-5	0.0	0.0	7.0328619868696345	0.0
18	6.0120722410600484E-5	0.0	0.0	7.501202414448212	0.0
19	6.0120722410600484E-5	0.0	0.0	7.740663251809634	0.0
20	6.0120722410600484E-5	0.0	0.0	8.032970204169974	0.0
21	6.0120722410600484E-5	0.0	0.0	8.364776471154077	0.0
22	6.0120722410600484E-5	0.0	0.0	8.68738426760936	0.0
23	6.0120722410600484E-5	0.0	0.0	9.032657576413438	0.0
24	6.0120722410600484E-5	0.0	0.0	9.28811052593608	0.0
25	6.0120722410600484E-5	0.0	0.0	9.510076233076017	0.0
26	6.0120722410600484E-5	0.0	0.0	9.74057908279826	0.0
27	6.0120722410600484E-5	0.0	0.0	9.971803381189428	0.0
28	6.0120722410600484E-5	0.0	0.0	10.228037900103407	0.0
29	6.0120722410600484E-5	0.0	0.0	10.47657696654883	0.0
30	6.0120722410600484E-5	0.0	0.0	10.809465406536326	0.0
31	6.0120722410600484E-5	0.0	0.0	11.123776543298945	0.0
32	6.0120722410600484E-5	0.0	0.0	11.397145468099945	0.0
33	6.0120722410600484E-5	0.0	0.0	11.651696606786427	0.0
34	6.0120722410600484E-5	0.0	0.0	11.9243440829185	0.0
35	6.0120722410600484E-5	0.0	0.0	12.262162422143664	0.0
36	6.0120722410600484E-5	0.0	0.0	12.55597239256427	0.0
37	6.0120722410600484E-5	0.0	0.0	12.835714113940794	0.0
38	6.0120722410600484E-5	0.0	0.0	13.089303321068705	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGATCA	65	0.0	44.000004	17
GCCCAAT	35	1.4466059E-7	44.0	9
ACCGCTC	20	7.8575E-4	44.0	18
CGACCGG	30	2.5284025E-6	44.0	33
GTTAGCG	35	1.4466059E-7	44.0	1
CCTCGCA	40	8.3164196E-9	44.0	37
CGTTAGT	30	2.5284025E-6	44.0	32
CGTTAGA	20	7.8575E-4	44.0	42
TATTGCG	70	0.0	44.0	1
AGTGCCG	20	7.8575E-4	44.0	1
CTACGGT	35	1.4466059E-7	44.0	3
ACTACGT	30	2.5284025E-6	44.0	22
TCCGTAG	90	0.0	44.0	34
CGTAATA	20	7.8575E-4	44.0	23
ACGTAAG	30	2.5284025E-6	44.0	1
TTCGATC	25	4.4437013E-5	44.0	27
GTAACGT	25	4.4437013E-5	44.0	31
CCGCAAT	20	7.8575E-4	44.0	33
TACGTTA	45	4.802132E-10	44.0	27
TACGTAC	50	2.7284841E-11	44.0	24
>>END_MODULE