FastQCFastQC Report
Thu 26 May 2016
SRR1546153_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546153_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2338973
Sequences flagged as poor quality0
Sequence length50
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT450301.925203924970489No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTATAGGACTCGTATGCC68200.29158096309790665No Hit
GAATCTGTCTCTTATACACATCTGACGCTATAGGACTCGTATGCCGTCTT57270.24485105214981104No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA54750.2340770928095365No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTATAGGACTCGTATG37540.16049779112456622No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTATAGGACTCGTATGC23850.10196782947045563No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTTAC207.858155E-444.00000416
CCCGTTA207.858155E-444.00000433
CCGTATG408.3182385E-944.00000442
CCCGTAG207.858155E-444.00000427
TGCCGAT207.858155E-444.00000418
CGTGATA254.4442575E-544.018
TTGCGTA302.5288464E-644.026
GGTTACG254.4442575E-544.01
CAATACG600.044.032
TACGTTA502.7284841E-1144.021
AGTCGAC502.7284841E-1144.027
GGCGTAA650.043.99999624
TACGTAG650.043.9999961
ACGGGTA1950.042.8717965
CGTTTTT254250.042.65881
TAGTACG1350.042.370371
TTGAGCG1750.041.4857141
TTCGTAC1650.041.33333633
GGCGATA3950.041.2151878
TGCACGG2300.041.1304362
Produced by FastQC (version 0.11.5)