##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546153_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2338973
Sequences flagged as poor quality	0
Sequence length	50
%GC	37
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.92445445073543	31.0	31.0	34.0	30.0	34.0
2	32.27486764490227	33.0	31.0	34.0	30.0	34.0
3	32.18795172069109	33.0	31.0	34.0	30.0	34.0
4	35.931218530526	37.0	35.0	37.0	35.0	37.0
5	35.91985713387884	37.0	35.0	37.0	35.0	37.0
6	35.97239771472351	37.0	35.0	37.0	35.0	37.0
7	36.182536523508396	37.0	35.0	37.0	35.0	37.0
8	36.0608514933691	37.0	35.0	37.0	35.0	37.0
9	37.79710753394759	39.0	38.0	39.0	35.0	39.0
10	37.51066130305908	39.0	37.0	39.0	35.0	39.0
11	37.41076275784287	39.0	37.0	39.0	35.0	39.0
12	37.040541724936546	39.0	35.0	39.0	33.0	39.0
13	36.913081083022334	39.0	35.0	39.0	33.0	39.0
14	38.15439383011262	40.0	37.0	41.0	33.0	41.0
15	38.24855011152331	40.0	37.0	41.0	33.0	41.0
16	38.32223629772554	40.0	36.0	41.0	34.0	41.0
17	38.17950399598456	40.0	36.0	41.0	34.0	41.0
18	37.97605059998555	39.0	36.0	41.0	34.0	41.0
19	37.76501823663634	39.0	36.0	41.0	34.0	41.0
20	37.43388316154141	39.0	35.0	41.0	33.0	41.0
21	37.40871656064435	39.0	35.0	41.0	33.0	41.0
22	37.33326079437428	39.0	35.0	41.0	33.0	41.0
23	37.284498367445885	39.0	35.0	41.0	33.0	41.0
24	37.23096547074293	39.0	35.0	41.0	33.0	41.0
25	37.228143291949074	39.0	35.0	41.0	33.0	41.0
26	37.16152986802327	39.0	35.0	41.0	33.0	41.0
27	37.08677269895805	39.0	35.0	41.0	33.0	41.0
28	37.017522647760366	39.0	35.0	41.0	33.0	41.0
29	36.935234395608674	39.0	35.0	41.0	33.0	41.0
30	36.74656398342349	39.0	35.0	41.0	32.0	41.0
31	36.461578650116955	39.0	35.0	41.0	31.0	41.0
32	36.1073214611712	39.0	35.0	41.0	30.0	41.0
33	35.70167120355814	39.0	35.0	41.0	27.0	41.0
34	35.233184820859414	38.0	35.0	41.0	23.0	41.0
35	34.98951633900862	38.0	35.0	41.0	22.0	41.0
36	34.83956462943352	38.0	35.0	41.0	21.0	41.0
37	34.785326722454684	38.0	35.0	41.0	21.0	41.0
38	34.732332951256815	38.0	35.0	41.0	20.0	41.0
39	34.63185509195703	38.0	35.0	41.0	19.0	41.0
40	34.53513657489847	38.0	34.0	41.0	18.0	41.0
41	34.42119639688017	38.0	34.0	41.0	18.0	41.0
42	34.384814617355566	38.0	34.0	41.0	18.0	41.0
43	34.262304866281056	38.0	34.0	40.0	18.0	41.0
44	34.12324981947205	38.0	34.0	40.0	17.0	41.0
45	34.06111742204805	38.0	34.0	40.0	18.0	41.0
46	34.017940352453834	38.0	34.0	40.0	18.0	41.0
47	33.95289385555113	38.0	34.0	40.0	18.0	41.0
48	33.88895639239957	38.0	34.0	40.0	17.0	41.0
49	33.83122891970108	37.0	34.0	40.0	17.0	41.0
50	33.67680131408101	37.0	33.0	40.0	15.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	3.0
11	7.0
12	5.0
13	11.0
14	16.0
15	46.0
16	95.0
17	303.0
18	729.0
19	1492.0
20	2865.0
21	4969.0
22	8524.0
23	13295.0
24	20411.0
25	32930.0
26	51230.0
27	61092.0
28	58662.0
29	51956.0
30	50029.0
31	52712.0
32	61946.0
33	79659.0
34	136951.0
35	257577.0
36	149539.0
37	178385.0
38	306056.0
39	757476.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	28.749583684805252	21.989864782534898	28.335598572535897	20.924952960123953
2	34.890911523989374	25.08626649388428	28.795159242966896	11.22766273915945
3	25.554848217572413	23.915068707505387	39.43769338081286	11.092389694109338
4	22.45485518644294	25.171261062013112	40.31521526755546	12.058668483988486
5	20.625847327010614	28.78814761863433	37.583247006271556	13.002758048083496
6	18.766698033709666	36.86511131167397	35.72559409621231	8.642596558404051
7	74.93374228774766	4.9557647736848605	18.08977700896932	2.020715929598161
8	75.35597888474985	4.820491728634747	17.19699201316133	2.6265373734540756
9	70.45263027833157	6.199986062258948	19.048402867412324	4.298980791997171
10	34.604204494878736	30.879193560592622	24.26017743684942	10.256424507679226
11	30.29068740853357	22.0023061403445	34.42698996525398	13.280016485867943
12	27.468850645133568	20.96086615792487	37.48546904987787	14.08481414706369
13	17.205713789770126	32.234189962859766	36.53308524724313	14.02701100012698
14	12.994592070964478	33.82847942237896	39.18386402921282	13.993064477443735
15	11.616893397230323	24.61533331081633	50.26240148988467	13.505371802068685
16	15.047031325286783	23.02134312794547	44.74570676959503	17.185918777172716
17	14.251169209734357	23.712073632316404	39.187583610413625	22.849173547535607
18	16.815371532719702	23.590268036441636	43.13316143452703	16.46119899631163
19	20.995838771973855	25.014611113510078	37.80804652298252	16.18150359153355
20	21.931121051846258	23.55927152643489	38.582788257923454	15.926819163795392
21	18.051426844174774	26.552422794106644	38.859405388604316	16.536744973114267
22	17.04282178545883	25.038895275832594	36.924325334238574	20.993957604469994
23	14.706711022316204	28.673310893285215	37.121292122653834	19.498685961744748
24	14.857802975921484	25.32979217802001	44.4612229384435	15.351181907615008
25	14.126798385445236	28.565400284654846	41.636820946629136	15.670980383270777
26	14.622657037939302	29.775290266283534	38.53533153225796	17.0667211635192
27	14.222780681948871	31.33187086811177	36.12183637861574	18.323512071323613
28	12.396765588999958	27.78501504720234	42.522380549070036	17.29583881472766
29	13.911917751936425	25.144155148434805	41.71886550208147	19.2250615975473
30	15.591501056232799	29.907057499167372	38.51472419732934	15.98671724727049
31	18.44441983725336	29.693074695603585	36.51286269657666	15.3496427705664
32	20.589164560685393	27.231267740157755	35.56834559441259	16.61122210474426
33	20.032424487157396	29.392729202089978	32.92397988347878	17.65086642727385
34	16.89523564401983	26.935881688245228	36.71560124892421	19.453281418810732
35	16.974586709637094	29.873581268360088	35.881773752839386	17.27005826916343
36	20.59053268250638	29.830314415771365	33.19020783908151	16.38894506264074
37	16.970012052298166	31.99361429140054	35.12545890867488	15.910914747626414
38	17.281045997538236	30.28525767505653	34.830585902445215	17.603110424960015
39	17.81760627420667	31.739271894117632	32.55099567203213	17.89212615964357
40	18.993036687469246	26.740197514037146	33.991243165269545	20.275522633224067
41	14.755664131223403	28.74808730156355	34.20364407797781	22.29260448923523
42	16.44623516389458	28.952022960504458	33.73690076798663	20.864841107614325
43	16.625117091988663	28.329784054796697	34.361149102618974	20.68394975059567
44	15.94353590229558	28.78040917958437	35.651587256458285	19.62446766166176
45	15.888212476159408	33.23753630332629	31.28479892670843	19.58945229380587
46	18.6368974759435	30.90702628888833	32.99828599988114	17.45779023528703
47	17.055476912302964	30.20385442670779	34.538662908892064	18.20200575209718
48	18.066561691819444	27.025750190361325	35.2922415094146	19.61544660840463
49	17.98383307545662	26.47080577672337	36.688452581538996	18.856908566281014
50	16.90400017443553	29.97383894555431	33.83023232846211	19.29192855154805
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	7888.0
1	11123.5
2	14359.0
3	45807.0
4	77255.0
5	53446.5
6	29638.0
7	30269.0
8	30900.0
9	31910.5
10	32921.0
11	32561.5
12	32202.0
13	30853.0
14	29504.0
15	29307.5
16	29111.0
17	26588.0
18	24065.0
19	24102.0
20	24139.0
21	24400.5
22	24662.0
23	29703.0
24	34744.0
25	39224.0
26	43704.0
27	47058.5
28	50413.0
29	63897.5
30	77382.0
31	90170.0
32	102958.0
33	112201.5
34	121445.0
35	128458.0
36	135471.0
37	137083.5
38	138696.0
39	148359.5
40	158023.0
41	167461.0
42	176899.0
43	184778.0
44	192657.0
45	180973.0
46	169289.0
47	152504.0
48	135719.0
49	115889.0
50	96059.0
51	82814.5
52	69570.0
53	62847.5
54	56125.0
55	49838.5
56	43552.0
57	41422.5
58	39293.0
59	39379.5
60	39466.0
61	35766.5
62	32067.0
63	28282.0
64	24497.0
65	19805.0
66	15113.0
67	12620.0
68	10127.0
69	8686.5
70	7246.0
71	6319.0
72	5392.0
73	4314.5
74	3237.0
75	2285.0
76	1333.0
77	1086.5
78	840.0
79	648.5
80	457.0
81	416.0
82	375.0
83	227.5
84	80.0
85	72.5
86	65.0
87	44.5
88	24.0
89	16.0
90	8.0
91	4.5
92	1.0
93	1.0
94	1.0
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	2338973.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	22.221172933820867
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.69913575457656	17.265659324108697
2	8.543321043900018	3.796852286913066
3	3.088518713686718	2.0589152533852357
4	1.6396243915212574	1.4573750860201868
5	1.0834908786446664	1.2038219093290323
6	0.7625544083947157	1.0166912028231863
7	0.5589079093422065	0.869371251530141
8	0.45521271308695516	0.8092288335343207
9	0.4065876329100986	0.8131368693283357
>10	4.4303788498186565	22.13770604422067
>50	0.7592776437298246	11.574741196033694
>100	0.5112924416830382	24.030146335203195
>500	0.053133911377137785	7.850559366733116
>1k	0.007785188480166709	2.3888587848253837
>5k	5.838891360125031E-4	0.779433566038256
>10k+	1.946297120041677E-4	1.947502689973514
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	45030	1.925203924970489	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTATAGGACTCGTATGCC	6820	0.29158096309790665	No Hit
GAATCTGTCTCTTATACACATCTGACGCTATAGGACTCGTATGCCGTCTT	5727	0.24485105214981104	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	5475	0.2340770928095365	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTATAGGACTCGTATG	3754	0.16049779112456622	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTATAGGACTCGTATGC	2385	0.10196782947045563	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.07614453009932137	0.0
2	0.0	0.0	0.0	0.3085969782464355	0.0
3	0.0	0.0	0.0	0.43134315787313493	0.0
4	0.0	0.0	0.0	0.7118508849824261	0.0
5	0.0	0.0	0.0	1.4358866049330197	0.0
6	0.0	0.0	0.0	1.9622287217509564	0.0
7	0.0	0.0	0.0	2.351160103173487	0.0
8	0.0	0.0	0.0	3.129706926929041	0.0
9	0.0	0.0	0.0	3.479005529349847	0.0
10	0.0	0.0	0.0	4.335407035480957	0.0
11	0.0	0.0	0.0	4.945845890482703	0.0
12	0.0	0.0	0.0	5.60626394575739	0.0
13	0.0	0.0	0.0	5.810840911801889	0.0
14	0.0	0.0	0.0	5.892457929185159	0.0
15	0.0	0.0	0.0	6.02734618997312	0.0
16	0.0	0.0	0.0	6.265142863983466	0.0
17	0.0	0.0	0.0	6.557536149412584	0.0
18	0.0	0.0	0.0	6.951897264312158	0.0
19	0.0	0.0	0.0	7.188625093149857	0.0
20	0.0	0.0	0.0	7.466824114686232	0.0
21	0.0	0.0	0.0	7.75797753971508	0.0
22	0.0	0.0	0.0	8.053876637310477	0.0
23	0.0	0.0	0.0	8.395436800681324	0.0
24	0.0	0.0	0.0	8.621005885916597	0.0
25	0.0	0.0	0.0	8.820324133711676	0.0
26	0.0	0.0	0.0	9.041703345870175	0.0
27	0.0	0.0	0.0	9.260773852455758	0.0
28	0.0	0.0	0.0	9.5416663638272	0.0
29	0.0	0.0	0.0	9.784336971824814	0.0
30	4.275380690585142E-5	0.0	0.0	10.101527465259325	0.0
31	4.275380690585142E-5	0.0	0.0	10.399478745586205	0.0
32	4.275380690585142E-5	0.0	0.0	10.649417500757812	0.0
33	4.275380690585142E-5	0.0	0.0	10.911412829476868	0.0
34	4.275380690585142E-5	0.0	0.0	11.183498056625707	0.0
35	4.275380690585142E-5	0.0	0.0	11.502697978984793	0.0
36	4.275380690585142E-5	0.0	0.0	11.789319500481621	0.0
37	4.275380690585142E-5	0.0	0.0	12.045457557654577	0.0
38	4.275380690585142E-5	0.0	0.0	12.288983241790307	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGTTAC	20	7.858155E-4	44.000004	16
CCCGTTA	20	7.858155E-4	44.000004	33
CCGTATG	40	8.3182385E-9	44.000004	42
CCCGTAG	20	7.858155E-4	44.000004	27
TGCCGAT	20	7.858155E-4	44.000004	18
CGTGATA	25	4.4442575E-5	44.0	18
TTGCGTA	30	2.5288464E-6	44.0	26
GGTTACG	25	4.4442575E-5	44.0	1
CAATACG	60	0.0	44.0	32
TACGTTA	50	2.7284841E-11	44.0	21
AGTCGAC	50	2.7284841E-11	44.0	27
GGCGTAA	65	0.0	43.999996	24
TACGTAG	65	0.0	43.999996	1
ACGGGTA	195	0.0	42.871796	5
CGTTTTT	25425	0.0	42.6588	1
TAGTACG	135	0.0	42.37037	1
TTGAGCG	175	0.0	41.485714	1
TTCGTAC	165	0.0	41.333336	33
GGCGATA	395	0.0	41.215187	8
TGCACGG	230	0.0	41.130436	2
>>END_MODULE