FastQCFastQC Report
Thu 26 May 2016
SRR1546152_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546152_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2379840
Sequences flagged as poor quality0
Sequence length50
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT453631.906136546994756No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTATAGGACTCGTATGCC66000.27732956837434447No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA55370.23266270001344627No Hit
GAATCTGTCTCTTATACACATCTGACGCTATAGGACTCGTATGCCGTCTT54760.2300995024875622No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTATAGGACTCGTATG35140.1476569853435525No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTATAGGACTCGTATGC24850.1044187844560979No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24070.10114125319349201No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTACGC207.858181E-444.00000435
TACGAAC207.858181E-444.00000441
TATAGCG950.044.0000041
AGTCGAC207.858181E-444.00000427
TCTATCG454.802132E-1044.00000422
TACTTCG302.5288646E-644.013
GAATACG502.7284841E-1144.01
CGACTAT351.4469515E-744.022
CGTTTTT251400.042.433571
CGACGGT1250.042.2428
AATACGG1250.042.242
CGCGTTA1100.042.018
GCTCGAT800.041.25000413
TTCGTAC2250.041.06666633
ATATCGG750.041.0666662
CCCTACA36650.040.99863427
CCCTAGC37150.040.7429321
ATTAGCG2000.040.71
TCGTTAA650.040.61538336
TTGGGAC22200.040.1351365