FastQCFastQC Report
Thu 26 May 2016
SRR1546151_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546151_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences4210181
Sequences flagged as poor quality0
Sequence length50
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT483911.149380513569369No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCC191940.4558948890795907No Hit
GAATCTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCTT141760.3367076142332123No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATG81540.19367338363837563No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA49760.1181896930321998No Hit
GCTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCTTCTG45550.10819012294245782No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAACCG207.8588736E-444.02
AACGCAT254.444868E-544.037
CCGAATA302.5293302E-644.025
ATCGACG302.5293302E-644.01
CGTTTTT332300.042.5633471
CTATGCG1500.042.5333331
GACGTAA2650.042.3396229
CACGTTA1000.041.843
ACGTAGC650.040.61538716
GGATGCC62950.040.6100048
TAATGCG1900.040.5263181
CGAGGGT4050.040.197534
CGACGAA557.8216544E-1140.019
GTGATCG1650.040.09
CGCTTAA1100.040.041
CGACGGT2150.039.90697528
GGGATGC76050.039.8921747
GTATGCG1600.039.8751
TAGGGAC47050.039.6046755
CTTAACG501.3496901E-939.61
Produced by FastQC (version 0.11.5)