FastQCFastQC Report
Thu 26 May 2016
SRR1546150_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546150_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences4319189
Sequences flagged as poor quality0
Sequence length50
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT494191.1441731306502216No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCC188970.43751269046110275No Hit
GAATCTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCTT138820.3214029300408016No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATG80940.18739629129449997No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA53160.12307866129497923No Hit
GCTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCTTCTG47100.10904824956722199No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATGCG2000.044.01
CGCGATA207.8588974E-444.011
TACGCTA254.444886E-544.033
ATAACCG302.5293466E-644.01
CACGTAT207.8588974E-444.018
GCTCGAA207.8588974E-444.040
GGCTCGA207.8588974E-444.039
TCGCGAT207.8588974E-444.012
CGTTTTT331400.042.519611
GCACGTT800.041.2542
CACGTTA800.041.2543
CACGTAA750.041.06666644
AACGCCC2600.040.61538335
GGATGCC61300.040.5546468
TGTTGCG2300.040.1739161
CTACGGG6100.040.0327873
TAGTACG1100.040.0000041
TTAGCGG7400.039.8378372
GATGCCA59700.039.835859
CGCAATA1050.039.80952517
Produced by FastQC (version 0.11.5)