##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546150_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	4319189
Sequences flagged as poor quality	0
Sequence length	50
%GC	38
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.796523375105835	31.0	31.0	34.0	30.0	34.0
2	32.20388804472321	33.0	31.0	34.0	30.0	34.0
3	32.27306885621351	34.0	31.0	34.0	30.0	34.0
4	35.949926016203506	37.0	35.0	37.0	35.0	37.0
5	35.85177425669495	37.0	35.0	37.0	35.0	37.0
6	35.92475879152313	37.0	35.0	37.0	35.0	37.0
7	36.136110737455574	37.0	35.0	37.0	35.0	37.0
8	36.01460019461987	37.0	35.0	37.0	35.0	37.0
9	37.717352030670575	39.0	38.0	39.0	35.0	39.0
10	37.43836354463766	39.0	37.0	39.0	34.0	39.0
11	37.29260122675808	39.0	37.0	39.0	34.0	39.0
12	36.989462142082694	39.0	35.0	39.0	33.0	39.0
13	36.83002225649306	39.0	35.0	39.0	33.0	39.0
14	38.04549534646435	40.0	36.0	41.0	33.0	41.0
15	38.190344529956896	40.0	37.0	41.0	33.0	41.0
16	38.26574965809554	40.0	36.0	41.0	34.0	41.0
17	38.170536413201646	40.0	36.0	41.0	34.0	41.0
18	38.03742276617208	39.0	36.0	41.0	34.0	41.0
19	37.90212977482578	40.0	36.0	41.0	34.0	41.0
20	37.6635815195862	39.0	35.0	41.0	34.0	41.0
21	37.60128348169066	39.0	35.0	41.0	33.0	41.0
22	37.540056709720275	39.0	35.0	41.0	33.0	41.0
23	37.45661882358007	39.0	35.0	41.0	33.0	41.0
24	37.415743094363314	39.0	35.0	41.0	33.0	41.0
25	37.41606769233761	39.0	35.0	41.0	33.0	41.0
26	37.361020321176035	39.0	35.0	41.0	33.0	41.0
27	37.29653738236507	39.0	35.0	41.0	33.0	41.0
28	37.210757852921	39.0	35.0	41.0	33.0	41.0
29	37.1260229640333	39.0	35.0	41.0	33.0	41.0
30	36.99488283564345	39.0	35.0	41.0	32.0	41.0
31	36.79688061809752	39.0	35.0	41.0	31.0	41.0
32	36.50936854117752	39.0	35.0	41.0	31.0	41.0
33	36.21817568066598	39.0	35.0	41.0	30.0	41.0
34	35.87669305510826	39.0	35.0	41.0	27.0	41.0
35	35.66463866248965	39.0	35.0	41.0	25.0	41.0
36	35.55001853357193	39.0	35.0	41.0	25.0	41.0
37	35.49032283606946	39.0	35.0	41.0	24.0	41.0
38	35.44835755045681	39.0	35.0	41.0	24.0	41.0
39	35.36590179313755	39.0	35.0	41.0	24.0	41.0
40	35.28380837235879	39.0	35.0	41.0	23.0	41.0
41	35.15854318947377	39.0	35.0	41.0	23.0	41.0
42	35.084700854720644	39.0	35.0	41.0	23.0	41.0
43	34.99474021627671	39.0	35.0	41.0	23.0	41.0
44	34.91017665584905	38.0	35.0	41.0	22.0	41.0
45	34.857269732813265	38.0	34.0	40.0	22.0	41.0
46	34.77365704533884	38.0	34.0	40.0	22.0	41.0
47	34.67737045079528	38.0	34.0	40.0	21.0	41.0
48	34.624253071583574	38.0	34.0	40.0	21.0	41.0
49	34.567831599867475	38.0	34.0	40.0	20.0	41.0
50	34.409708628170705	38.0	34.0	40.0	20.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	0.0
9	1.0
10	6.0
11	7.0
12	4.0
13	14.0
14	23.0
15	59.0
16	189.0
17	433.0
18	1057.0
19	2303.0
20	4414.0
21	7482.0
22	12981.0
23	20162.0
24	31108.0
25	50131.0
26	78172.0
27	95588.0
28	94379.0
29	87373.0
30	87820.0
31	96781.0
32	117081.0
33	149879.0
34	253089.0
35	437007.0
36	256441.0
37	330407.0
38	586197.0
39	1518600.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	29.47717731268532	23.590354578139554	29.30645081750301	17.626017291672117
2	33.07000457724818	26.634282500719465	29.15644580498793	11.139267117044426
3	24.177849128621137	26.05831789254881	38.29901863521138	11.464814343618675
4	22.971395787496217	26.466797354781185	38.313859384250144	12.24794747347245
5	20.574788461444964	30.28550035666418	36.661975199510834	12.477735982380025
6	20.49442615268746	38.37935779147428	32.125012357643996	9.001203698194267
7	79.21345882294106	4.063424869807735	14.539025729135723	2.184090578115475
8	78.97737283550222	4.569005894393601	13.51316184589283	2.940459424211351
9	72.84809717750254	6.671761758978363	15.431971140878531	5.04816992264057
10	33.68018857243802	34.51645667739939	21.651402612851626	10.151952137310962
11	28.2171490990554	22.511749312197267	35.19910798068804	14.071993608059291
12	26.77944864186309	22.774946870813014	35.41523651778146	15.03036796954243
13	17.056627991967936	31.49489406460333	37.20862874951756	14.239849193911173
14	13.021171335637316	33.586907171693575	36.979581120437196	16.412340372231917
15	11.477293538208215	26.3070451420394	47.88651295416801	14.329148365584373
16	13.418329228010165	25.328528110254034	42.927711660684444	18.325431001051353
17	13.713315161712073	25.405880594713498	37.17339065273597	23.707413590838467
18	15.67266447474283	27.16491452446281	39.89630923768328	17.266111763111084
19	18.20357942197019	28.190986780157107	35.369857628364954	18.23557616950775
20	20.282534522105884	26.73191657044876	37.59490496942829	15.390643938017067
21	16.46174779570887	28.618335525488696	38.10854306213504	16.81137361666739
22	17.28641650087551	25.17831472528755	35.20820691106594	22.327061862770996
23	14.762539912006629	28.906699845734927	35.72388242329752	20.60687781896092
24	14.192803324883444	26.137082679178892	43.61747077981538	16.052643216122288
25	13.835815010642044	31.016771898613378	39.34583552606751	15.801577564677071
26	14.242071833392798	30.663163848583615	36.82976132787891	18.265002990144676
27	14.365821917031182	31.56622690046673	35.67679024928059	18.391160933221492
28	12.456227314896385	29.141906038378963	40.69808012569026	17.703786521034388
29	13.817362472445637	26.351220101736693	38.845973167647905	20.985444258169764
30	14.774741276660967	30.891748427771976	36.54209158247069	17.79141871309637
31	17.29079232235496	30.622276543119554	34.08250947110673	18.004421663418757
32	18.512665224883655	28.41415367560901	34.950126053756854	18.123055045750487
33	17.547136742569034	29.60134414122651	33.36216590660885	19.4893532095956
34	15.472603768901985	28.0653150394669	34.969713064188674	21.492368127442443
35	16.381223419489167	27.400884749428656	36.09029380284123	20.12759802824095
36	18.75847526005461	29.386465838841502	33.545348443886105	18.30971045721778
37	15.419677166245794	30.5193173996322	36.801723656918	17.25928177720401
38	14.706858162493006	29.62880299982242	35.810773735532294	19.853565102152277
39	16.182135118421538	30.698841842762608	34.79697230197614	18.322050736839717
40	17.51215332322804	27.959461834154514	34.79048960348806	19.737895239129383
41	14.569540717018867	29.288438176704005	34.034167062381385	22.107854043895742
42	16.49751839986627	28.606759278188566	33.59132929816223	21.304393023782936
43	16.335520395148258	28.515723669420346	34.17171603280153	20.97703990262987
44	16.108695405549515	29.66135540723038	33.11123917013125	21.11871001708886
45	15.205308218741992	32.65652417618215	32.06090310009587	20.077264504979986
46	17.961404328451476	31.122578798936562	32.71933689403265	18.19667997857931
47	16.183732640548957	29.540754062857633	34.76810114120961	19.5074121553838
48	16.17787505941509	27.213465305639556	36.0164141925718	20.592245442373557
49	16.80507613813612	27.08714529510054	35.84522464749748	20.262553919265862
50	15.974549851835611	29.955067953729277	34.43806233068291	19.632319863752198
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	7841.0
1	11807.0
2	15773.0
3	52392.0
4	89011.0
5	63521.0
6	38031.0
7	39223.0
8	40415.0
9	43095.5
10	45776.0
11	45791.5
12	45807.0
13	43062.0
14	40317.0
15	38783.5
16	37250.0
17	34829.0
18	32408.0
19	33296.5
20	34185.0
21	38144.5
22	42104.0
23	54966.0
24	67828.0
25	85619.5
26	103411.0
27	119208.0
28	135005.0
29	156308.0
30	177611.0
31	204737.0
32	231863.0
33	254736.0
34	277609.0
35	274739.5
36	271870.0
37	281989.5
38	292109.0
39	298199.0
40	304289.0
41	317839.0
42	331389.0
43	320722.0
44	310055.0
45	326894.0
46	343733.0
47	313580.0
48	283427.0
49	256446.5
50	229466.0
51	188517.5
52	147569.0
53	120680.0
54	93791.0
55	78046.0
56	62301.0
57	55997.5
58	49694.0
59	44828.5
60	39963.0
61	37286.0
62	34609.0
63	28902.0
64	23195.0
65	18236.5
66	13278.0
67	11798.0
68	10318.0
69	8352.5
70	6387.0
71	5100.0
72	3813.0
73	3320.5
74	2828.0
75	2138.0
76	1448.0
77	1091.5
78	735.0
79	507.5
80	280.0
81	258.5
82	237.0
83	145.5
84	54.0
85	38.0
86	22.0
87	24.5
88	27.0
89	17.5
90	8.0
91	8.5
92	9.0
93	15.0
94	21.0
95	20.0
96	19.0
97	9.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	4319189.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	17.86744547064125
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.6272062734353	13.512649841875435
2	9.615916384030058	3.4362372328380566
3	3.976414941346942	2.131451313994788
4	2.1614342384815943	1.5447723357778755
5	1.307822512118171	1.1683723710274239
6	0.8826248297870929	0.9462150610352937
7	0.6245945844245724	0.7811936774924716
8	0.49484576149097664	0.7073303727854376
9	0.3930000055578818	0.6319715552340445
>10	3.284343408902357	12.910788729796579
>50	0.8162773654964608	10.216865384791598
>100	0.6532957026882308	24.755292171005618
>500	0.11799301700998585	14.543530078952346
>1k	0.043574729307358506	10.471238472989091
>5k	2.624983693214368E-4	0.31447625386386147
>10k+	3.937475539821552E-4	1.9276151465400213
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	49419	1.1441731306502216	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCC	18897	0.43751269046110275	No Hit
GAATCTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCTT	13882	0.3214029300408016	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATG	8094	0.18739629129449997	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	5316	0.12307866129497923	No Hit
GCTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCTTCTG	4710	0.10904824956722199	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.11830924740732578	0.0
2	0.0	0.0	0.0	0.47872413084956456	0.0
3	0.0	0.0	0.0	0.681678898515439	0.0
4	0.0	0.0	0.0	1.071659517562209	0.0
5	0.0	0.0	0.0	2.1061592812910015	0.0
6	0.0	0.0	0.0	2.800479441858182	0.0
7	0.0	0.0	0.0	3.3118717425887128	0.0
8	0.0	0.0	0.0	4.242764092981345	0.0
9	0.0	0.0	0.0	4.583082611110558	0.0
10	0.0	0.0	0.0	5.604848502809208	0.0
11	0.0	0.0	0.0	6.263745346637991	0.0
12	0.0	0.0	0.0	7.031759897517798	0.0
13	0.0	0.0	0.0	7.282617176511609	0.0
14	2.315249460025945E-5	0.0	0.0	7.376569999599462	0.0
15	2.315249460025945E-5	0.0	0.0	7.570773124306438	0.0
16	2.315249460025945E-5	0.0	0.0	7.902502066938956	0.0
17	2.315249460025945E-5	0.0	0.0	8.285212802681244	0.0
18	2.315249460025945E-5	0.0	0.0	8.81123747999914	0.0
19	2.315249460025945E-5	0.0	0.0	9.115137124122144	0.0
20	2.315249460025945E-5	0.0	0.0	9.404057104238781	0.0
21	2.315249460025945E-5	0.0	0.0	9.796399277734778	0.0
22	2.315249460025945E-5	0.0	0.0	10.216709664707889	0.0
23	2.315249460025945E-5	0.0	0.0	10.676981257361046	0.0
24	2.315249460025945E-5	0.0	0.0	11.012229379172803	0.0
25	2.315249460025945E-5	0.0	0.0	11.29302283368475	0.0
26	2.315249460025945E-5	0.0	0.0	11.572658663466683	0.0
27	2.315249460025945E-5	0.0	0.0	11.85789739694188	0.0
28	2.315249460025945E-5	0.0	0.0	12.150845911118962	0.0
29	2.315249460025945E-5	0.0	0.0	12.461783913600447	0.0
30	2.315249460025945E-5	0.0	0.0	12.862229460206533	0.0
31	2.315249460025945E-5	0.0	0.0	13.225839387903608	0.0
32	2.315249460025945E-5	0.0	0.0	13.548168417728421	0.0
33	2.315249460025945E-5	0.0	0.0	13.849660202412998	0.0
34	2.315249460025945E-5	0.0	0.0	14.183333954591939	0.0
35	2.315249460025945E-5	0.0	0.0	14.606839385819884	0.0
36	2.315249460025945E-5	0.0	0.0	14.946787464035493	0.0
37	2.315249460025945E-5	0.0	0.0	15.273307095382952	0.0
38	4.63049892005189E-5	0.0	0.0	15.573571797853717	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTATGCG	200	0.0	44.0	1
CGCGATA	20	7.8588974E-4	44.0	11
TACGCTA	25	4.444886E-5	44.0	33
ATAACCG	30	2.5293466E-6	44.0	1
CACGTAT	20	7.8588974E-4	44.0	18
GCTCGAA	20	7.8588974E-4	44.0	40
GGCTCGA	20	7.8588974E-4	44.0	39
TCGCGAT	20	7.8588974E-4	44.0	12
CGTTTTT	33140	0.0	42.51961	1
GCACGTT	80	0.0	41.25	42
CACGTTA	80	0.0	41.25	43
CACGTAA	75	0.0	41.066666	44
AACGCCC	260	0.0	40.615383	35
GGATGCC	6130	0.0	40.554646	8
TGTTGCG	230	0.0	40.173916	1
CTACGGG	610	0.0	40.032787	3
TAGTACG	110	0.0	40.000004	1
TTAGCGG	740	0.0	39.837837	2
GATGCCA	5970	0.0	39.83585	9
CGCAATA	105	0.0	39.809525	17
>>END_MODULE