FastQCFastQC Report
Thu 26 May 2016
SRR1546149_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546149_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences597935
Sequences flagged as poor quality0
Sequence length50
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATCTGTCTCTTATACACATCTGACGCCCAAATGCTCGTATGCCGTCTT356325.95917616463328No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCCAAATGCTCGTATGCC284804.76305952988201No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT202843.3923419769707412No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCCAAATGCTCGTATG141452.3656417503574803No Hit
GAACTGTCTCTTATACACATCTGACGCCCAAATGCTCGTATGCCGTCTTC97941.637970682432037No Hit
GAATGACTGTCTCTTATACACATCTGACGCCCAAATGCTCGTATGCCGTC66351.1096523869651382No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCCAAATGCTCGTATGCCGT61821.0338916437405403No Hit
GCTGTCTCTTATACACATCTGACGCCCAAATGCTCGTATGCCGTCTTCTG52580.8793597966334132No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA47410.7928955488472827No Hit
GAATGCTGTCTCTTATACACATCTGACGCCCAAATGCTCGTATGCCGTCT38570.6450533920911136No Hit
GAATCTGTCTCTTATACACATCTGACGCCCAAATGCTCGTATGCCGTCCT30200.5050716214973199No Hit
GAATGATACCTGGCTCTTATACACATCTGACGCCCAAATGCTCGTATGCC22340.3736192061009976No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCCAAATGCTCGT18100.3027084883808441No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCCAAATGC14640.2448426668450584No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCCCAATGCTCGTATG12920.21607699833593952No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCCCAAATGCTCG12460.20838385443233795No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA11730.19617516954183986No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCCCAATGCTCGTATGCC10580.17694230978283593No Hit
GAATGATACTGTCTCTTATACACATCTGACGCCCAAATGCTCGTATGCCG8480.14182143544030706No Hit
CGCTGTCTCTTATACACATCTGACGCCCAAATGCTCGTATGCCGTCTTCT7440.12442824052781656No Hit
CTGTCTCTTATACACATCTGACGCCCAAATGCTCGTATGCCGTCTTCTGC7190.1202471840584679Illumina Single End Adapter 1 (95% over 21bp)
GAATCTGTCTCTTATACACATCTGACGCCCCAATGCTCGTATGCCGTCTT6600.11037989079080501No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGAAC207.853447E-444.00000417
TGCGGGA408.301868E-944.0000044
GTTCGAA302.5256704E-644.00000415
AGTGACT207.853447E-444.0000042
TTAACGG302.5256704E-644.0000042
CGATGCG207.853447E-444.00000410
TATACTA207.853447E-444.00000444
ATTGCGG207.853447E-444.0000042
CGCCGGT302.5256704E-644.00000428
TACCGTA302.5256704E-644.00000429
TCGCCTA254.4402695E-544.030
CCGTGTA351.4445322E-744.031
CCGCGTA254.4402695E-544.031
TGTACGG254.4402695E-544.02
GCTCGAA351.4445322E-744.042
CGATAAG254.4402695E-544.010
CGTTTTT72050.043.297711
CGGCTAT1100.042.031
ATACCGG1050.041.9047628
CAATGGG850.041.4117663
Produced by FastQC (version 0.11.5)