##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546149_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	597935
Sequences flagged as poor quality	0
Sequence length	50
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.14929047471715	30.0	28.0	31.0	16.0	33.0
2	28.011160075927986	30.0	27.0	31.0	16.0	33.0
3	27.743585841270374	30.0	26.0	31.0	16.0	33.0
4	32.38848035321565	35.0	32.0	35.0	28.0	35.0
5	33.26066880179284	35.0	33.0	35.0	28.0	37.0
6	33.4538536797478	35.0	33.0	35.0	28.0	37.0
7	33.55511050532248	35.0	33.0	35.0	29.0	37.0
8	33.12622609480964	35.0	32.0	35.0	28.0	37.0
9	33.62647110471874	35.0	32.0	39.0	27.0	39.0
10	34.27411675182085	35.0	32.0	39.0	28.0	39.0
11	34.35003303034611	37.0	32.0	39.0	27.0	39.0
12	33.70586936707167	35.0	32.0	39.0	27.0	39.0
13	33.64538453176348	35.0	32.0	39.0	25.0	39.0
14	35.59936615183925	37.0	34.0	40.0	29.0	41.0
15	35.0104626757089	37.0	32.0	40.0	27.0	41.0
16	35.17330144580933	36.0	33.0	40.0	27.0	41.0
17	34.414046677314424	36.0	32.0	39.0	26.0	41.0
18	34.03262394741903	36.0	32.0	39.0	25.0	40.0
19	33.14521478087083	35.0	31.0	38.0	24.0	40.0
20	32.97115405520667	35.0	31.0	38.0	24.0	40.0
21	33.75994882386882	35.0	32.0	38.0	25.0	40.0
22	34.31067256474366	36.0	33.0	39.0	27.0	40.0
23	34.390027344109306	35.0	33.0	39.0	27.0	40.0
24	34.747009290307474	36.0	33.0	39.0	27.0	40.0
25	34.97055198307508	36.0	33.0	39.0	27.0	40.0
26	34.21690819236205	36.0	33.0	39.0	25.0	40.0
27	34.06252519086523	36.0	33.0	39.0	24.0	40.0
28	33.971217607265004	36.0	33.0	39.0	24.0	40.0
29	33.86844054955806	36.0	33.0	39.0	24.0	40.0
30	33.66046643865972	36.0	33.0	39.0	22.0	40.0
31	32.944144430414674	35.0	31.0	39.0	21.0	40.0
32	32.496284713221335	35.0	31.0	39.0	16.0	40.0
33	32.12709240971009	35.0	30.0	39.0	15.0	40.0
34	31.552439646449866	35.0	30.0	39.0	12.0	40.0
35	31.327803189309876	35.0	30.0	39.0	9.0	40.0
36	31.221219697793238	35.0	28.0	39.0	10.0	40.0
37	31.079500280130784	35.0	27.0	39.0	9.0	40.0
38	30.795063008520994	35.0	26.0	39.0	9.0	40.0
39	30.34443877679012	35.0	25.0	38.0	9.0	40.0
40	30.20190154448226	35.0	25.0	38.0	8.0	40.0
41	29.794409091289186	34.0	25.0	38.0	8.0	40.0
42	29.908270965907665	35.0	24.0	38.0	8.0	40.0
43	30.20117571307918	35.0	25.0	38.0	8.0	40.0
44	30.472405863513593	35.0	26.0	38.0	8.0	40.0
45	30.322046710762876	35.0	25.0	39.0	8.0	40.0
46	29.870522715679797	35.0	24.0	38.0	8.0	40.0
47	29.955764422554292	35.0	25.0	38.0	8.0	40.0
48	30.010265329843545	35.0	25.0	38.0	8.0	40.0
49	29.707598652027393	35.0	24.0	38.0	7.0	40.0
50	29.79647118833987	35.0	24.0	38.0	8.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	1.0
11	1.0
12	1.0
13	6.0
14	17.0
15	71.0
16	236.0
17	709.0
18	1918.0
19	3891.0
20	5993.0
21	7650.0
22	9580.0
23	12027.0
24	14947.0
25	18551.0
26	22000.0
27	24062.0
28	24558.0
29	25737.0
30	29222.0
31	34908.0
32	41599.0
33	47979.0
34	56677.0
35	61333.0
36	53842.0
37	51502.0
38	41979.0
39	6937.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	51.56480219421844	14.53519195230251	12.561064329734837	21.33894152374422
2	26.30804351643573	51.891760810121504	13.13955530283392	8.660640370608846
3	13.538762574527333	50.469532641507854	27.66220408572838	8.32950069823643
4	13.951516469181433	14.28399407962404	61.42172644183732	10.342763009357204
5	38.71624842165119	16.301939173990483	28.461287598150303	16.520524806208034
6	11.768670507663877	46.427120004682784	32.6659252259861	9.138284261667238
7	35.29698044101784	8.917858964603177	49.41674262252586	6.368417971853129
8	28.6480972012008	32.378435783153684	28.721683795061338	10.251783220584178
9	27.897848428340872	8.40442523016716	25.41045431359596	38.28727202789601
10	33.29609405704633	15.245804309833009	33.373694465117445	18.08440716800321
11	30.429896226178432	16.24507680600734	34.440198349318905	18.884828618495323
12	17.9945980750416	14.281485445742431	38.84636289897732	28.877553580238658
13	24.753359478873122	18.795186767792487	44.66672798882822	11.784725764506176
14	12.355356351442882	39.08100378803716	29.98068351911161	18.582956341408348
15	9.142967044912908	19.712845041685135	46.88302240210056	24.261165511301396
16	9.869467417026936	23.89707911395051	30.417353056770384	35.81610041225217
17	9.998745683059196	31.12395160009031	36.74145183004842	22.13585088680208
18	9.815782651960497	25.358943697893583	35.128901970950025	29.6963716791959
19	11.297214580180121	26.47361335262194	28.932074556598963	33.29709751059898
20	20.45924724259326	25.65061419719535	38.02620686194988	15.863931698261515
21	13.717210064639133	36.616689105003054	34.275297482167794	15.39080334819002
22	10.745984095261191	19.334877536856013	41.65921044929633	28.25992791858647
23	21.57876692282606	27.395285440725164	36.92558555695854	14.100362079490244
24	19.85968374488866	28.751118432605548	29.757415103648388	21.6317827188574
25	12.356359804995526	43.48215106993235	27.916245076806007	16.245244048266116
26	13.546455718430932	18.47107127028858	43.10635771446729	24.876115296813197
27	27.32086263557076	21.636298259844295	27.940829688845774	23.102009415739168
28	11.12562402267805	28.861498323396358	36.19490412837516	23.817973525550435
29	18.801374731367122	18.553856188381683	35.36889461229064	27.275874467960566
30	11.963173254617978	34.965506284127876	30.461839497604252	22.609480963649894
31	15.363208375492318	26.361226554725846	26.279277847926615	31.99628722185522
32	25.985767683778338	31.249216051912	25.95031232491826	16.814703939391407
33	19.53088546413908	27.029693863045313	26.345004055624777	27.09441661719083
34	16.876583575137765	21.946699892128745	34.62433207622902	26.55238445650447
35	24.845342721198794	21.326732838853722	26.77464941841505	27.053275021532443
36	15.39548613143569	30.89048140684188	26.151504762223322	27.56252769949911
37	13.058442807328555	25.652453862041863	35.51305743935377	25.776045891275807
38	15.84419711172619	30.491274135148473	22.35376754998453	31.310761203140807
39	19.45345229832674	24.892505038173045	33.21096774733039	22.443074916169817
40	19.561658039753485	24.222198065007067	32.66542349920978	23.55072039602967
41	13.49042956174166	25.3286728490555	28.863170745984096	32.31772684321875
42	20.12794032796207	21.938003294672498	38.538469900574476	19.395586476790953
43	21.634124110480236	23.122747455827138	25.36680408405596	29.87632434963667
44	19.326515423917314	20.71613135207004	30.905867694649086	29.051485529363557
45	18.26954434846597	19.89530634600751	31.76800153862878	30.06714776689774
46	28.317292013345934	25.89478789500531	24.985826218568906	20.802093873079848
47	13.67038223218243	24.19610827263833	40.55557878364705	21.57793071153219
48	20.009031081973795	22.036508985090354	27.98247301128049	29.971986921655365
49	18.02135683644543	18.86994405746444	37.378644835977155	25.730054270112973
50	16.78861414702267	18.939851321631952	34.53703161714902	29.73450291419636
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	5810.0
1	4690.5
2	3571.0
3	15379.5
4	27188.0
5	17641.0
6	8094.0
7	7607.0
8	7120.0
9	6976.0
10	6832.0
11	6716.0
12	6600.0
13	6286.0
14	5972.0
15	5617.5
16	5263.0
17	4921.0
18	4579.0
19	4245.5
20	3912.0
21	3585.0
22	3258.0
23	3060.5
24	2863.0
25	2776.0
26	2689.0
27	3032.0
28	3375.0
29	3306.5
30	3238.0
31	4034.0
32	4830.0
33	5487.0
34	6144.0
35	7513.0
36	8882.0
37	10471.5
38	12061.0
39	14000.0
40	15939.0
41	19274.0
42	22609.0
43	28424.0
44	34239.0
45	80906.5
46	127574.0
47	104610.5
48	81647.0
49	68562.0
50	55477.0
51	44654.5
52	33832.0
53	29330.5
54	24829.0
55	22232.0
56	19635.0
57	17364.5
58	15094.0
59	13287.5
60	11481.0
61	9848.0
62	8215.0
63	6849.0
64	5483.0
65	4288.5
66	3094.0
67	2626.5
68	2159.0
69	1864.0
70	1569.0
71	1366.0
72	1163.0
73	1001.5
74	840.0
75	599.5
76	359.0
77	279.5
78	200.0
79	147.5
80	95.0
81	78.5
82	62.0
83	41.0
84	20.0
85	20.5
86	21.0
87	13.5
88	6.0
89	5.5
90	5.0
91	3.0
92	1.0
93	2.0
94	3.0
95	3.0
96	3.0
97	1.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	597935.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	34.978165088367
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.95153102040055	27.965578510992668
2	6.808551945213839	4.76301307904824
3	2.958607016278469	3.1045993394096763
4	1.9175380128095683	2.6828784470108915
5	1.427675922135482	2.4968742048570745
6	1.0968664613519397	2.301982569903664
7	0.9263376112787505	2.2681112926410174
8	0.7541595034794321	2.110329249253157
9	0.635264674896988	1.999835340601918
>10	3.375288384419502	18.386905863499802
>50	0.07051626451722315	1.7879006029280535
>100	0.06575165204979679	4.645741723265926
>500	0.0033352287271636053	0.770123852174338
>1k	0.004764612467376579	3.648963805097843
>5k	0.0019058449869506315	4.644575121455665
>10k+	0.0019058449869506315	16.422586997860083
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATCTGTCTCTTATACACATCTGACGCCCAAATGCTCGTATGCCGTCTT	35632	5.95917616463328	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCCAAATGCTCGTATGCC	28480	4.76305952988201	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	20284	3.3923419769707412	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCCAAATGCTCGTATG	14145	2.3656417503574803	No Hit
GAACTGTCTCTTATACACATCTGACGCCCAAATGCTCGTATGCCGTCTTC	9794	1.637970682432037	No Hit
GAATGACTGTCTCTTATACACATCTGACGCCCAAATGCTCGTATGCCGTC	6635	1.1096523869651382	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCCAAATGCTCGTATGCCGT	6182	1.0338916437405403	No Hit
GCTGTCTCTTATACACATCTGACGCCCAAATGCTCGTATGCCGTCTTCTG	5258	0.8793597966334132	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	4741	0.7928955488472827	No Hit
GAATGCTGTCTCTTATACACATCTGACGCCCAAATGCTCGTATGCCGTCT	3857	0.6450533920911136	No Hit
GAATCTGTCTCTTATACACATCTGACGCCCAAATGCTCGTATGCCGTCCT	3020	0.5050716214973199	No Hit
GAATGATACCTGGCTCTTATACACATCTGACGCCCAAATGCTCGTATGCC	2234	0.3736192061009976	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCCAAATGCTCGT	1810	0.3027084883808441	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCCAAATGC	1464	0.2448426668450584	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCCCAATGCTCGTATG	1292	0.21607699833593952	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCCCAAATGCTCG	1246	0.20838385443233795	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1173	0.19617516954183986	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCCCAATGCTCGTATGCC	1058	0.17694230978283593	No Hit
GAATGATACTGTCTCTTATACACATCTGACGCCCAAATGCTCGTATGCCG	848	0.14182143544030706	No Hit
CGCTGTCTCTTATACACATCTGACGCCCAAATGCTCGTATGCCGTCTTCT	744	0.12442824052781656	No Hit
CTGTCTCTTATACACATCTGACGCCCAAATGCTCGTATGCCGTCTTCTGC	719	0.1202471840584679	Illumina Single End Adapter 1 (95% over 21bp)
GAATCTGTCTCTTATACACATCTGACGCCCCAATGCTCGTATGCCGTCTT	660	0.11037989079080501	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.16038532616421516	0.0
2	0.0	0.0	0.0	1.495982004732956	0.0
3	0.0	0.0	0.0	1.8062163947586276	0.0
4	1.67242258773947E-4	0.0	0.0	3.950262152240628	0.0
5	1.67242258773947E-4	0.0	0.0	11.900122923060199	0.0
6	1.67242258773947E-4	0.0	0.0	13.11413447950028	0.0
7	1.67242258773947E-4	0.0	0.0	14.796257118248638	0.0
8	1.67242258773947E-4	0.0	0.0	16.393086205022286	0.0
9	1.67242258773947E-4	0.0	0.0	16.771053709851405	0.0
10	1.67242258773947E-4	0.0	0.0	22.61901377240001	0.0
11	1.67242258773947E-4	0.0	0.0	22.88559793288568	0.0
12	1.67242258773947E-4	0.0	0.0	26.194151538210676	0.0
13	5.01726776321841E-4	0.0	0.0	26.424109644024853	0.0
14	6.68969035095788E-4	0.0	0.0	26.658917775343475	0.0
15	8.36211293869735E-4	0.0	0.0	27.269352019868382	0.0
16	8.36211293869735E-4	0.0	0.0	27.694481841671752	0.0
17	8.36211293869735E-4	0.0	0.0	27.883465594086314	0.0
18	0.001003453552643682	0.0	0.0	28.00705762332026	0.0
19	0.001170695811417629	0.0	0.0	28.649100654753443	0.0
20	0.001505180328965523	0.0	0.0	28.817346367080034	0.0
21	0.001505180328965523	0.0	0.0	28.898626104844173	0.0
22	0.001505180328965523	0.0	0.0	29.107678928311607	0.0
23	0.001839664846513417	0.0	0.0	29.240971008554443	0.0
24	0.001839664846513417	0.0	0.0	29.60405395235268	0.0
25	0.001839664846513417	0.0	0.0	29.725471832222567	0.0
26	0.001839664846513417	0.0	0.0	30.028682047379732	0.0
27	0.002006907105287364	0.0	0.0	30.678418222716516	0.0
28	0.002006907105287364	0.0	0.0	30.82559141043759	0.0
29	0.002341391622835258	0.0	0.0	31.04083219747966	0.0
30	0.002341391622835258	0.0	0.0	31.152382784081883	0.0
31	0.002508633881609205	0.0	0.0	31.295709399851155	0.0
32	0.002508633881609205	0.0	0.0	31.54372966961292	0.0
33	0.002508633881609205	0.0	0.0	31.621831804460353	0.0
34	0.002508633881609205	0.0	0.0	31.833894988585715	0.0
35	0.002508633881609205	0.0	0.0	31.922031658959586	0.0
36	0.002508633881609205	0.0	0.0	31.98708889762265	0.0
37	0.002675876140383152	0.0	0.0	32.06619448602273	0.0
38	0.002675876140383152	0.0	0.0	32.156672548019436	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACCGAAC	20	7.853447E-4	44.000004	17
TGCGGGA	40	8.301868E-9	44.000004	4
GTTCGAA	30	2.5256704E-6	44.000004	15
AGTGACT	20	7.853447E-4	44.000004	2
TTAACGG	30	2.5256704E-6	44.000004	2
CGATGCG	20	7.853447E-4	44.000004	10
TATACTA	20	7.853447E-4	44.000004	44
ATTGCGG	20	7.853447E-4	44.000004	2
CGCCGGT	30	2.5256704E-6	44.000004	28
TACCGTA	30	2.5256704E-6	44.000004	29
TCGCCTA	25	4.4402695E-5	44.0	30
CCGTGTA	35	1.4445322E-7	44.0	31
CCGCGTA	25	4.4402695E-5	44.0	31
TGTACGG	25	4.4402695E-5	44.0	2
GCTCGAA	35	1.4445322E-7	44.0	42
CGATAAG	25	4.4402695E-5	44.0	10
CGTTTTT	7205	0.0	43.29771	1
CGGCTAT	110	0.0	42.0	31
ATACCGG	105	0.0	41.90476	28
CAATGGG	85	0.0	41.411766	3
>>END_MODULE