FastQCFastQC Report
Thu 26 May 2016
SRR1546148_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546148_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences608457
Sequences flagged as poor quality0
Sequence length50
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATCTGTCTCTTATACACATCTGACGCCCAAATGCTCGTATGCCGTCTT356505.8590828932858035No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCCAAATGCTCGTATGCC276414.5428025316497305No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT210153.453818429239864No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCCAAATGCTCGTATG144232.3704222319736643No Hit
GAACTGTCTCTTATACACATCTGACGCCCAAATGCTCGTATGCCGTCTTC95901.576117950816574No Hit
GAATGACTGTCTCTTATACACATCTGACGCCCAAATGCTCGTATGCCGTC64371.057921923817131No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCCAAATGCTCGTATGCCGT59610.9796912518057973No Hit
GCTGTCTCTTATACACATCTGACGCCCAAATGCTCGTATGCCGTCTTCTG55210.907377185240699No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA46110.7578185475719731No Hit
GAATGCTGTCTCTTATACACATCTGACGCCCAAATGCTCGTATGCCGTCT39830.6546066525654237No Hit
GAATGATACCTGGCTCTTATACACATCTGACGCCCAAATGCTCGTATGCC21610.35516067692540315No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCCAAATGCTCGT21030.34562836815091286No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCCAAATGC16270.2673976961395793No Hit
GAATCTGTCTCTTATACACATCTGACGCCCAAATGCTCGTATGCCGTCCT15580.2560575357009616No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCCCAAATGCTCG14270.23452766588271645No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCCCAATGCTCGTATG13190.21677784954401053No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA12430.20428723804640264No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCCCAATGCTCGTATGCC10410.17108850748697113No Hit
GAATCTGTCTCTTATACACATCTGACGCCCCAATGCTCGTATGCCGTCTT8470.13920457813781417No Hit
CGCTGTCTCTTATACACATCTGACGCCCAAATGCTCGTATGCCGTCTTCT7290.1198112602862651No Hit
CTGTCTCTTATACACATCTGACGCCCAAATGCTCGTATGCCGTCTTCTGC7250.11915385968112784Illumina Single End Adapter 1 (95% over 21bp)
GAATGATACTGTCTCTTATACACATCTGACGCCCAAATGCTCGTATGCCG7070.11619555695801018No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGACC207.8535563E-444.00000410
ATAGCGG207.8535563E-444.0000042
ATATCGT207.8535563E-444.00000428
ACGGCTT207.8535563E-444.00000430
AATTGCG207.8535563E-444.0000041
CGGGCGA207.8535563E-444.0000046
AATCTAG207.8535563E-444.00000411
CGGCTTA207.8535563E-444.00000431
CGTCGAC207.8535563E-444.0000049
GTGCGAC207.8535563E-444.00000410
GCGTATA207.8535563E-444.00000433
ACCGGTC302.525745E-644.017
GCGACGG351.4445868E-744.037
CCGTGTA454.783942E-1044.031
CCAATCG351.4445868E-744.024
TATATCG254.440362E-544.027
AGGTTCG351.4445868E-744.025
TGCTCGG254.440362E-544.02
CGGCTAT1400.044.031
CCGGTCT302.525745E-644.018
Produced by FastQC (version 0.11.5)