FastQCFastQC Report
Thu 26 May 2016
SRR1546147_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546147_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences604197
Sequences flagged as poor quality0
Sequence length50
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATCTGTCTCTTATACACATCTGACGCCCAAATGCTCGTATGCCGTCTT361285.979506684078206No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCCAAATGCTCGTATGCC276404.574666871897741No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT205803.406173814169881No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCCAAATGCTCGTATG140062.3181180972431177No Hit
GAACTGTCTCTTATACACATCTGACGCCCAAATGCTCGTATGCCGTCTTC95241.576307065410785No Hit
GAATGACTGTCTCTTATACACATCTGACGCCCAAATGCTCGTATGCCGTC67731.120991994332974No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCCAAATGCTCGTATGCCGT57850.9574691698237495No Hit
GCTGTCTCTTATACACATCTGACGCCCAAATGCTCGTATGCCGTCTTCTG56020.9271810353245712No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA45570.7542242017090452No Hit
GAATGCTGTCTCTTATACACATCTGACGCCCAAATGCTCGTATGCCGTCT38370.6350577708926063No Hit
GAATGATACCTGGCTCTTATACACATCTGACGCCCAAATGCTCGTATGCC22830.37785689104712533No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCCAAATGCTCGT20270.3354866045346137No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCCAAATGC17800.29460589840730755No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCCCAAATGCTCG13640.2257541828244761No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA12470.2063896378168048No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCCCAATGCTCGTATG12270.20307945918301482No Hit
GAATCTGTCTCTTATACACATCTGACGCCCCAATGCTCGTATGCCGTCTT10940.1810667712683115No Hit
GAATCTGTCTCTTATACACATCTGACGCCCAAATGCTCGTATGCCGTCCT10470.17328785147890505No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCCCAATGCTCGTATGCC9400.1555783957881287No Hit
CGCTGTCTCTTATACACATCTGACGCCCAAATGCTCGTATGCCGTCTTCT7600.12578678808401897No Hit
GAATGATACTGTCTCTTATACACATCTGACGCCCAAATGCTCGTATGCCG7540.12479373449388197No Hit
CTGTCTCTTATACACATCTGACGCCCAAATGCTCGTATGCCGTCTTCTGC6720.11122200209534308Illumina Single End Adapter 1 (95% over 21bp)
GAACTGTCTCTTATACACATCTGACGCCCAAATGCTCGTATGCCGTCCTC6260.10360859123762613No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCCCAAATGCTCGTA6100.10096044833059416No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGGGA207.853513E-444.04
CCGATTC207.853513E-444.040
CAACGAT302.525716E-644.016
TAGACGG351.4445504E-744.02
ACCGCGT351.4445504E-744.030
CCGTGTA600.044.031
GGATCGA207.853513E-444.08
CCAGCGC254.440325E-544.019
CCTCGTA207.853513E-444.040
ATACTCC207.853513E-444.028
AGCGGGT351.4445504E-744.04
ATATCGC207.853513E-444.028
GGAACTA254.440325E-544.08
GCGGGAT254.440325E-544.05
AGCGCAG408.301868E-944.01
GCCTCAC254.440325E-544.09
CGGGATC254.440325E-544.06
TCCAATC207.853513E-444.023
CGTGTTT302.525716E-644.01
CGGTCTA551.8189894E-1244.031
Produced by FastQC (version 0.11.5)