##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546147_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	604197
Sequences flagged as poor quality	0
Sequence length	50
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.08833542702132	30.0	28.0	31.0	16.0	33.0
2	27.910037620180173	30.0	27.0	31.0	16.0	33.0
3	27.718906250775824	30.0	26.0	31.0	16.0	33.0
4	32.390925476293326	35.0	32.0	35.0	28.0	35.0
5	33.29479457858943	35.0	33.0	35.0	28.0	37.0
6	33.515158135508784	35.0	33.0	35.0	29.0	37.0
7	33.56741427051111	35.0	33.0	35.0	30.0	37.0
8	33.13033828370548	35.0	32.0	35.0	28.0	37.0
9	33.67683057016172	35.0	32.0	39.0	27.0	39.0
10	34.326103903197136	35.0	32.0	39.0	28.0	39.0
11	34.486139454515666	37.0	33.0	39.0	27.0	39.0
12	33.86545447925097	35.0	32.0	39.0	27.0	39.0
13	33.74629632388112	35.0	32.0	39.0	25.0	39.0
14	35.650458376986315	37.0	34.0	40.0	30.0	41.0
15	35.07370940272792	37.0	33.0	40.0	27.0	41.0
16	35.18923960231514	36.0	33.0	40.0	27.0	41.0
17	34.410298296747584	36.0	32.0	39.0	25.0	41.0
18	34.082971282545266	36.0	32.0	39.0	26.0	40.0
19	33.01782696703228	35.0	31.0	38.0	24.0	40.0
20	32.85180661274386	35.0	30.0	38.0	24.0	40.0
21	33.67505962459264	35.0	32.0	38.0	25.0	40.0
22	34.12401915269358	35.0	32.0	39.0	26.0	40.0
23	34.26067160214301	35.0	32.0	39.0	26.0	40.0
24	34.40868623975293	36.0	33.0	39.0	26.0	40.0
25	34.85034351378772	36.0	33.0	39.0	27.0	40.0
26	34.0628437413625	35.0	33.0	39.0	25.0	40.0
27	33.88876641227944	36.0	33.0	39.0	24.0	40.0
28	33.792006580635125	36.0	33.0	39.0	24.0	40.0
29	33.70007298943887	35.0	32.0	39.0	24.0	40.0
30	33.49702001168493	36.0	33.0	39.0	21.0	40.0
31	32.84328290276185	35.0	31.0	38.0	20.0	40.0
32	32.33008935827222	35.0	30.0	39.0	16.0	40.0
33	31.970062744436003	35.0	30.0	39.0	15.0	40.0
34	31.23903958477119	35.0	29.0	39.0	10.0	40.0
35	30.93413241045553	35.0	27.0	39.0	8.0	40.0
36	30.939038095190806	35.0	27.0	39.0	10.0	40.0
37	30.844558976625173	35.0	26.0	39.0	8.0	40.0
38	30.59783481215564	35.0	25.0	39.0	9.0	40.0
39	30.04343781912191	35.0	25.0	38.0	8.0	40.0
40	29.94438403368438	35.0	25.0	38.0	8.0	40.0
41	29.548761413909702	34.0	24.0	38.0	8.0	40.0
42	29.561681041117385	35.0	23.0	38.0	8.0	40.0
43	29.89330135700773	35.0	24.0	38.0	8.0	40.0
44	30.25171756893861	35.0	25.0	38.0	8.0	40.0
45	30.107287854789085	35.0	24.0	38.0	8.0	40.0
46	29.725392545808734	35.0	24.0	38.0	8.0	40.0
47	29.848466642502363	35.0	25.0	38.0	8.0	40.0
48	29.822946820325157	35.0	24.0	38.0	8.0	40.0
49	29.738300587391198	35.0	24.0	38.0	7.0	40.0
50	29.689288427449988	35.0	24.0	38.0	7.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	2.0
12	5.0
13	5.0
14	19.0
15	75.0
16	253.0
17	688.0
18	1691.0
19	3638.0
20	5693.0
21	7749.0
22	10310.0
23	13165.0
24	16715.0
25	20377.0
26	23883.0
27	25263.0
28	25467.0
29	26631.0
30	29735.0
31	35004.0
32	40829.0
33	47812.0
34	55415.0
35	60434.0
36	52891.0
37	51148.0
38	42117.0
39	7183.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	52.30876684260266	14.213741544562453	12.325450142916962	21.152041469917922
2	26.03339639223631	52.61032411614093	12.845479206285368	8.510800285337398
3	13.222012025878977	51.28708020728339	27.217943816338046	8.272963950499589
4	13.640087587326649	13.998910951229481	62.10954374152801	10.251457719915855
5	39.92555408252606	15.989983399454152	28.11202306532472	15.972439452695063
6	11.493767761177232	47.53714434199441	32.01406163883634	8.955026257992014
7	34.23717760928969	8.828577434181236	50.62256184655005	6.31168310997903
8	28.03903362644965	33.841280244688406	28.136683896146458	9.983002232715489
9	27.293084871325078	8.283391013196027	25.228691966361964	39.19483214911693
10	34.765813137105944	14.946118567288483	32.61485906086922	17.673209234736355
11	32.04683240731086	15.9608538274768	33.70821106361005	18.284102701602293
12	17.597902670817632	13.978056825836607	37.99191323359765	30.43212726974811
13	26.516516963837955	18.37645668548503	43.54788256148243	11.559143789194584
14	12.137431996517693	40.31416905413301	29.575618548254955	17.972780401094347
15	8.954860749060323	19.34716656984394	45.69420238763185	26.003770293463884
16	9.667045682120236	23.244901911131635	30.02083757449971	37.067214832248425
17	9.858208498221606	32.89953442337516	36.05281058992348	21.189446488479753
18	9.689720405761697	24.558380793019495	34.49123381943306	31.26066498178574
19	11.155467504803896	25.655705010120872	28.700241808549197	34.488585676526036
20	22.500608245323956	24.815581672864976	37.43116897303363	15.252641108777434
21	13.703311999232037	37.844610284394	33.67726089338411	14.774816822989854
22	10.602171146165903	19.009859367060745	42.99524823857119	27.392721248202157
23	23.204021205004327	26.54779815192727	36.29346057660002	13.954720066468388
24	19.517640769484128	30.180388184648383	29.329175748969295	20.972795296898198
25	12.195194613677327	44.26370869103951	27.652735779886363	15.888360915396799
26	13.268851053547104	18.068113545747497	44.370627460910924	24.292407939794472
27	28.401001660054586	21.112484835244132	27.789611666393576	22.696901838307703
28	11.202306532472024	29.872376062774226	35.63837622497298	23.28694117978077
29	18.43041259721581	18.04974205432996	36.88416195379984	26.635683394654393
30	11.763712828762804	35.660223403955996	30.326532571330212	22.24953119595099
31	15.064788471309853	25.506250444805257	26.03256884757786	33.39639223630703
32	26.97034245453056	30.5678445937335	26.007908016756126	16.453904934979814
33	20.902122983066782	26.49169062408453	26.192947002384987	26.413239390463705
34	16.536162874029497	21.545125182680483	34.45200820262265	27.466703740667363
35	25.206017242720502	20.996297565198105	27.096460260477954	26.701224931603434
36	15.36634574484812	31.363777046228297	26.056402133741148	27.21347507518243
37	12.878415483691578	25.32154247703977	35.40103641693024	26.39900562233841
38	15.552708801930496	30.182870818623726	22.274357535704414	31.990062843741363
39	18.989501768462937	25.564840606623335	32.80006355542977	22.64559406948396
40	19.1003927526949	23.98207869287666	32.28218610817333	24.635342446255112
41	13.33687522447149	24.884267879516116	28.696104085256962	33.08275281075544
42	20.138630281183122	22.187134328704047	38.11538289663802	19.55885249347481
43	21.12688411230112	23.4140520393183	25.46255608683923	29.996507761541352
44	18.952758785627864	20.497288136154268	31.33431645638757	29.2156366218303
45	18.332762327519003	20.00854026087518	31.905156761784646	29.75354064982117
46	28.199577290188465	26.125915885050738	24.85431076288032	20.82019606188048
47	13.580669880850122	25.25749052047594	39.992254181996934	21.169585416677013
48	19.76011135440924	22.003419414528704	28.423179856900976	29.81328937416108
49	18.425281820333435	18.969144169865125	37.626800530290616	24.97877347951082
50	16.75264855667936	19.259943362843575	34.137044705617534	29.850363374859523
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	5658.0
1	4627.5
2	3597.0
3	15765.5
4	27934.0
5	18099.5
6	8265.0
7	7656.5
8	7048.0
9	6964.0
10	6880.0
11	6620.5
12	6361.0
13	6203.0
14	6045.0
15	5552.5
16	5060.0
17	4710.0
18	4360.0
19	4071.5
20	3783.0
21	3486.0
22	3189.0
23	2954.5
24	2720.0
25	2725.5
26	2731.0
27	3018.0
28	3305.0
29	3304.0
30	3303.0
31	4007.5
32	4712.0
33	5346.5
34	5981.0
35	7441.0
36	8901.0
37	10419.5
38	11938.0
39	13984.5
40	16031.0
41	19409.5
42	22788.0
43	28364.0
44	33940.0
45	80390.5
46	126841.0
47	104023.5
48	81206.0
49	69145.0
50	57084.0
51	46638.0
52	36192.0
53	31509.0
54	26826.0
55	24039.5
56	21253.0
57	18694.5
58	16136.0
59	14082.0
60	12028.0
61	9998.5
62	7969.0
63	6563.5
64	5158.0
65	4010.5
66	2863.0
67	2418.0
68	1973.0
69	1712.5
70	1452.0
71	1294.5
72	1137.0
73	955.0
74	773.0
75	571.5
76	370.0
77	280.0
78	190.0
79	136.5
80	83.0
81	69.0
82	55.0
83	48.5
84	42.0
85	30.5
86	19.0
87	11.0
88	3.0
89	5.0
90	7.0
91	5.5
92	4.0
93	2.5
94	1.0
95	1.5
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	604197.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	34.92443057647502
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.60801247109185	27.802645048778054
2	6.968217071972751	4.867220267438407
3	3.031744681534833	3.176458699675819
4	1.973800387493449	2.7573541841933777
5	1.4144843870353034	2.4700030888261137
6	1.15389171468263	2.417940664932194
7	0.9220165266444112	2.254063152260876
8	0.7354684346303074	2.054865302914794
9	0.6444873813682422	2.0257519327208415
>10	3.3881667503216932	18.52734435194043
>50	0.08269018879971782	2.054870137356475
>100	0.06520712031053114	4.607615572202333
>500	0.0033076075519834634	0.8077890557619862
>1k	0.0047251536456906626	3.3768717973559808
>5k	0.001890061458276265	4.568505049992814
>10k+	0.001890061458276265	16.230701693649518
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATCTGTCTCTTATACACATCTGACGCCCAAATGCTCGTATGCCGTCTT	36128	5.979506684078206	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCCAAATGCTCGTATGCC	27640	4.574666871897741	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	20580	3.406173814169881	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCCAAATGCTCGTATG	14006	2.3181180972431177	No Hit
GAACTGTCTCTTATACACATCTGACGCCCAAATGCTCGTATGCCGTCTTC	9524	1.576307065410785	No Hit
GAATGACTGTCTCTTATACACATCTGACGCCCAAATGCTCGTATGCCGTC	6773	1.120991994332974	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCCAAATGCTCGTATGCCGT	5785	0.9574691698237495	No Hit
GCTGTCTCTTATACACATCTGACGCCCAAATGCTCGTATGCCGTCTTCTG	5602	0.9271810353245712	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	4557	0.7542242017090452	No Hit
GAATGCTGTCTCTTATACACATCTGACGCCCAAATGCTCGTATGCCGTCT	3837	0.6350577708926063	No Hit
GAATGATACCTGGCTCTTATACACATCTGACGCCCAAATGCTCGTATGCC	2283	0.37785689104712533	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCCAAATGCTCGT	2027	0.3354866045346137	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCCAAATGC	1780	0.29460589840730755	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCCCAAATGCTCG	1364	0.2257541828244761	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1247	0.2063896378168048	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCCCAATGCTCGTATG	1227	0.20307945918301482	No Hit
GAATCTGTCTCTTATACACATCTGACGCCCCAATGCTCGTATGCCGTCTT	1094	0.1810667712683115	No Hit
GAATCTGTCTCTTATACACATCTGACGCCCAAATGCTCGTATGCCGTCCT	1047	0.17328785147890505	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCCCAATGCTCGTATGCC	940	0.1555783957881287	No Hit
CGCTGTCTCTTATACACATCTGACGCCCAAATGCTCGTATGCCGTCTTCT	760	0.12578678808401897	No Hit
GAATGATACTGTCTCTTATACACATCTGACGCCCAAATGCTCGTATGCCG	754	0.12479373449388197	No Hit
CTGTCTCTTATACACATCTGACGCCCAAATGCTCGTATGCCGTCTTCTGC	672	0.11122200209534308	Illumina Single End Adapter 1 (95% over 21bp)
GAACTGTCTCTTATACACATCTGACGCCCAAATGCTCGTATGCCGTCCTC	626	0.10360859123762613	No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCCCAAATGCTCGTA	610	0.10096044833059416	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.1444892973649323	0.0
2	0.0	0.0	0.0	1.43926566997188	0.0
3	0.0	0.0	0.0	1.7505879704798268	0.0
4	0.0	0.0	0.0	3.833186857928788	0.0
5	0.0	0.0	0.0	11.470596510740702	0.0
6	0.0	0.0	0.0	12.734422713121713	0.0
7	0.0	0.0	0.0	14.367168324238618	0.0
8	0.0	0.0	0.0	15.898953487024928	0.0
9	0.0	0.0	0.0	16.263735172468582	0.0
10	1.655089316894986E-4	0.0	0.0	21.834765813137107	0.0
11	1.655089316894986E-4	0.0	0.0	22.0886565143488	0.0
12	3.310178633789972E-4	0.0	0.0	25.307474217846167	0.0
13	3.310178633789972E-4	0.0	0.0	25.537035106099502	0.0
14	3.310178633789972E-4	0.0	0.0	25.772554315893657	0.0
15	4.965267950684959E-4	0.0	0.0	26.417046095892566	0.0
16	4.965267950684959E-4	0.0	0.0	26.84405913965147	0.0
17	4.965267950684959E-4	0.0	0.0	27.030753214597226	0.0
18	4.965267950684959E-4	0.0	0.0	27.14826455609677	0.0
19	8.275446584474931E-4	0.0	0.0	27.859290926634856	0.0
20	9.930535901369918E-4	0.0	0.0	28.019503572510292	0.0
21	0.0011585625218264904	0.0	0.0	28.093320556043807	0.0
22	0.0011585625218264904	0.0	0.0	28.32701916758938	0.0
23	0.0013240714535159889	0.0	0.0	28.461246911189562	0.0
24	0.0013240714535159889	0.0	0.0	28.84787577561623	0.0
25	0.0013240714535159889	0.0	0.0	28.96737322429605	0.0
26	0.0013240714535159889	0.0	0.0	29.31378341832217	0.0
27	0.0013240714535159889	0.0	0.0	30.01338967257368	0.0
28	0.0014895803852054876	0.0	0.0	30.149438014422447	0.0
29	0.0014895803852054876	0.0	0.0	30.38578476887505	0.0
30	0.0014895803852054876	0.0	0.0	30.500482458535874	0.0
31	0.0014895803852054876	0.0	0.0	30.65026804171487	0.0
32	0.0014895803852054876	0.0	0.0	30.90448976078994	0.0
33	0.0014895803852054876	0.0	0.0	30.985092610522727	0.0
34	0.0014895803852054876	0.0	0.0	31.204391945011313	0.0
35	0.0014895803852054876	0.0	0.0	31.296580419962364	0.0
36	0.0014895803852054876	0.0	0.0	31.366094171271953	0.0
37	0.0014895803852054876	0.0	0.0	31.454475940794143	0.0
38	0.0014895803852054876	0.0	0.0	31.544181781769854	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTGGGA	20	7.853513E-4	44.0	4
CCGATTC	20	7.853513E-4	44.0	40
CAACGAT	30	2.525716E-6	44.0	16
TAGACGG	35	1.4445504E-7	44.0	2
ACCGCGT	35	1.4445504E-7	44.0	30
CCGTGTA	60	0.0	44.0	31
GGATCGA	20	7.853513E-4	44.0	8
CCAGCGC	25	4.440325E-5	44.0	19
CCTCGTA	20	7.853513E-4	44.0	40
ATACTCC	20	7.853513E-4	44.0	28
AGCGGGT	35	1.4445504E-7	44.0	4
ATATCGC	20	7.853513E-4	44.0	28
GGAACTA	25	4.440325E-5	44.0	8
GCGGGAT	25	4.440325E-5	44.0	5
AGCGCAG	40	8.301868E-9	44.0	1
GCCTCAC	25	4.440325E-5	44.0	9
CGGGATC	25	4.440325E-5	44.0	6
TCCAATC	20	7.853513E-4	44.0	23
CGTGTTT	30	2.525716E-6	44.0	1
CGGTCTA	55	1.8189894E-12	44.0	31
>>END_MODULE