FastQCFastQC Report
Thu 26 May 2016
SRR1546146_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546146_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences472532
Sequences flagged as poor quality0
Sequence length50
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT93881.9867437549203018No Hit
GAATGATACGGCTGCCTCTTATACACATCTGACGCCCAAATGCTCGTATG66681.411121363209264No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCCAAATGCTCGTATGCC58771.243725292678591No Hit
GAATCTGTCTCTTATACACATCTGACGCCCAAATGCTCGTATGCCGTCTT57411.214944173093039No Hit
GAATGATACCTGTCCCTTATACACATCTGACGCCCAAATGCTCGTATGCC56241.1901839452142924No Hit
GAATCTTTCTCTTACCCACATCTGACGCCCAAATGCTCGTATGCCGTCTT42580.9011029940829405No Hit
GAACTGTCCCTTATCCACATCTGACGCCCAAATGCTCGTATGCCGTCTTC38460.8139131318090626No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCCAAATGCTCGTATG25480.5392227404704866No Hit
GAACTGTCCCTTATACACATCTGACGCCCAAATGCTCGTATGCCGTCTTC23410.49541618345424226No Hit
GAATCTGTCTCTTACCCACATCTGACGCCCAAATGCTCGTATGCCGTCTT23030.4873744000406322No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA21650.4581700286964693No Hit
GAATGACTCTCTCTCCTACACATCTGACGCCCAAATGCTCGTATGCCGTC19210.4065333141459203No Hit
GCTGTCTCCTATACCCATCTGACGCCCAAATGCTCGTATGCCGTCTTCTG18960.4012426671632821No Hit
GAATGATACGGCTGCCTCTTATACACATCTGACGCCCCAATGCTCGTATG17620.3728847993363412No Hit
GAATGACTCTCTCTTATACACATCTGACGCCCAAATGCTCGTATGCCGTC16320.34537343502662254No Hit
GAATGATACCTGGCCCTTATACACATCTGACGCCCAAATGCTCGTATGCC14490.3066458991137108No Hit
GAATGATCCGTCTCCCATACACATCTGACGCCCAAATGCTCGTATGCCGT13920.2945832239932957No Hit
GAATGCTGCCTCTTCCACACATCTGACGCCCAAATGCTCGTATGCCGTCT13660.28908095113135196No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCCCAATGCTCGTATGCC13430.2842135559073248No Hit
GAATCTGTCTCTTATACACATCTGACGCCCAAATGCTCGTATGCCGTCCT11330.2397721212531638No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCCCAATGCTCGTATG10920.23109546020163713No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCCAAATGC10890.23046058256372057No Hit
GAATCTTTCTCTTATACACATCTGACGCCCAAATGCTCGTATGCCGTCTT10350.21903278508122204No Hit
GCTGTCTCTTATACACATCTGACGCCCAAATGCTCGTATGCCGTCTTCTG10310.21818628156399988No Hit
GCTGTCTCCTATACACATCTGACGCCCAAATGCTCGTATGCCGTCTTCTG8920.18877028434053145No Hit
GAATCTGTCTCTTATACACATCTGACGCCCCAATGCTCGTATGCCGTCTT8170.17289834339261678No Hit
GAATCTTTCTCTTACCCCCATCTGACGCCCAAATGCTCGTATGCCGTCTT7670.16231704942734038No Hit
CGTTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7640.1616821717894238No Hit
GAATGATACGGCGACCGTCTCTTATACACATCTGACGCCCCAATGCTCGT7240.15321713661720265No Hit
GAATGCTGCCTCTTATACACATCTGACGCCCAAATGCTCGTATGCCGTCT7140.15110087782414736No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCCCAAATGCTCG6730.14242421677262068No Hit
GAACTGTCTCTTATACACATCTGACGCCCAAATGCTCGTATGCCGTCTTC6700.1417893391347041No Hit
GAACTGTCCCTTATACACATCTGACGCCCAAATGCTCGTATGCCGTCCTC6700.1417893391347041No Hit
GAATCTTTCTCTTACCCACATCTGACGCCCCAATGCTCGTATGCCGTCTT6630.1403079579795654No Hit
GAATGATACGGCGACCGTCTCTTATACACATCTGACGCCCAAATGCTCGT5890.1246476429109563No Hit
GAATGATCCGTCTCTTATACACATCTGACGCCCAAATGCTCGTATGCCGT5740.12147325472137335No Hit
GAACTGTCCCTTATCCACATCTGACGCCCACATGCTCGTATGCCGTCTTC5470.11575935598012409No Hit
GAATCTGTCTCTTATACACATCTGACGCCCCAATGCTCGTATGCCGTCCT5360.11343147130776328No Hit
GAATCTGTCTCTTACACACATCTGACGCCCAAATGCTCGTATGCCGTCTT5170.10941057960095824No Hit
GAATGATACCTGTCCCTTATACACATCTGACGCCCCAATGCTCGTATGCC5000.10581293965276427No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACGAC207.851768E-444.00000425
GGTAATT207.851768E-444.0000048
ACCGCGT408.294592E-944.00000430
CGGTTTT408.294592E-944.0000041
TACGGAT207.851768E-444.0000047
GACCACG207.851768E-444.00000413
TGCGCGC207.851768E-444.00000415
TACGAGG207.851768E-444.0000042
CGGGCTT207.851768E-444.0000046
ACTACTA207.851768E-444.00000435
CGGGATC207.851768E-444.0000046
AGATTGG207.851768E-444.0000042
CTCGACC207.851768E-444.00000432
TCGACGG207.851768E-444.0000042
ATCGCTA207.851768E-444.00000413
GCATCGC207.851768E-444.00000422
TCGCCTA254.438849E-544.030
CACGGGA254.438849E-544.04
CGACGGT254.438849E-544.028
CGCGCCT254.438849E-544.017
Produced by FastQC (version 0.11.5)