##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546146_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	472532
Sequences flagged as poor quality	0
Sequence length	50
%GC	44
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	21.318291671251895	16.0	16.0	28.0	16.0	31.0
2	21.429651325201256	16.0	16.0	30.0	10.0	31.0
3	22.190048504651536	25.0	16.0	30.0	10.0	31.0
4	26.754257912691628	28.0	19.0	33.0	19.0	35.0
5	30.72179450280616	32.0	28.0	35.0	19.0	35.0
6	31.237080240068398	33.0	30.0	35.0	23.0	35.0
7	30.00287599569976	32.0	28.0	35.0	17.0	35.0
8	30.364180626920504	32.0	28.0	35.0	17.0	35.0
9	23.197326318640854	19.0	17.0	31.0	16.0	35.0
10	25.114542507174118	27.0	17.0	34.0	17.0	37.0
11	27.464368550701327	30.0	17.0	35.0	17.0	39.0
12	27.135832070632254	29.0	17.0	37.0	15.0	39.0
13	28.13280370429939	31.0	17.0	37.0	15.0	39.0
14	30.92502095096205	32.0	27.0	37.0	17.0	40.0
15	28.19318268392405	30.0	17.0	36.0	17.0	40.0
16	29.2316858964049	31.0	25.0	36.0	17.0	39.0
17	27.603620495543158	30.0	17.0	36.0	16.0	39.0
18	27.974935030855054	31.0	17.0	36.0	16.0	39.0
19	26.841995885992905	30.0	18.0	36.0	10.0	38.0
20	26.771939254907604	30.0	18.0	35.0	10.0	38.0
21	29.01289648108488	32.0	25.0	36.0	15.0	38.0
22	30.664767677109698	33.0	27.0	36.0	15.0	38.0
23	30.943938188313172	34.0	27.0	37.0	18.0	39.0
24	31.953046566158484	35.0	30.0	37.0	19.0	39.0
25	31.891698340006602	34.0	30.0	37.0	21.0	39.0
26	25.375125917398186	32.0	15.0	36.0	0.0	38.0
27	27.918629849407026	32.0	18.0	36.0	10.0	39.0
28	29.023448147427054	33.0	24.0	36.0	13.0	39.0
29	29.747995902922977	33.0	25.0	36.0	14.0	38.0
30	29.735137514496373	33.0	25.0	36.0	11.0	38.0
31	28.168773755004953	32.0	23.0	36.0	9.0	38.0
32	27.74913445015364	32.0	21.0	36.0	8.0	38.0
33	28.000649691449468	32.0	23.0	36.0	9.0	38.0
34	27.20972124639178	31.0	19.0	36.0	8.0	38.0
35	26.89833281132283	32.0	18.0	36.0	7.0	38.0
36	27.482041427882134	32.0	20.0	36.0	8.0	39.0
37	27.207006086360288	32.0	18.0	36.0	8.0	39.0
38	26.50625566099227	31.0	18.0	36.0	8.0	38.0
39	25.90177384812034	30.0	16.0	35.0	8.0	38.0
40	25.895820812135476	30.0	16.0	35.0	8.0	38.0
41	25.02750713179213	29.0	15.0	35.0	8.0	38.0
42	25.01592273115895	29.0	15.0	35.0	7.0	38.0
43	25.71391355506082	30.0	15.0	35.0	7.0	38.0
44	26.278082754183842	31.0	18.0	35.0	7.0	38.0
45	25.666710402681723	30.0	15.0	35.0	7.0	38.0
46	24.574090220344864	28.0	14.0	34.0	7.0	37.0
47	24.828100530757705	29.0	15.0	34.0	7.0	37.0
48	25.203802070547603	29.0	16.0	34.0	7.0	37.0
49	24.819550845233763	28.0	15.0	34.0	7.0	37.0
50	26.122186433934633	30.0	17.0	36.0	7.0	39.0
>>END_MODULE
>>Per sequence quality scores	warn
#Quality	Count
11	7.0
12	42.0
13	181.0
14	851.0
15	2515.0
16	5239.0
17	8536.0
18	11639.0
19	14120.0
20	15476.0
21	17277.0
22	19474.0
23	23185.0
24	27769.0
25	33249.0
26	37285.0
27	37949.0
28	36500.0
29	33468.0
30	30187.0
31	28228.0
32	25650.0
33	23147.0
34	19612.0
35	13852.0
36	6009.0
37	1060.0
38	25.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	57.75820473534068	11.525568638737695	9.95297672961831	20.763249896303318
2	25.72418375898352	56.163180483014905	10.745727273496822	7.366908484504753
3	11.860784031557651	54.14977186730211	27.178053549812496	6.811390551327741
4	12.718715346262263	10.881379462131665	67.12921029686878	9.270694894737288
5	44.201450907028516	12.981131436601121	28.82111687674062	13.996300779629738
6	10.361837928436593	50.54514826509104	31.732242472467476	7.360771334004893
7	31.710445006899	4.349123445607916	59.60061964057461	4.339811906918474
8	28.873600094808396	35.25221572295633	27.074356868952794	8.799827313282487
9	21.674722558472233	2.8736678150897714	15.9163823825688	59.535227243869194
10	37.760828896244064	11.963634208900139	33.08961086233314	17.185926032522666
11	36.182311462504124	13.232966232974697	33.862468573556924	16.72225373096425
12	15.243623712256523	11.120939957505524	39.62906215875327	34.00637417148468
13	28.188567123496398	17.174921486798777	44.04929189980785	10.587219489896981
14	11.37912353025827	40.43916602473484	30.85632295802189	17.325387486985008
15	5.703317447283993	13.915882945493637	34.97604395046262	45.404755656759754
16	6.319572007821693	15.488263228733715	25.662389002226305	52.52977576121829
17	7.602448088171807	29.73428254594398	37.04701480534652	25.616254560537698
18	7.286279024489346	22.153420297461334	33.37826856170587	37.18203211634344
19	9.090178019689672	23.41830817807048	29.79544242506328	37.69607137717657
20	16.811983103789796	22.693912793207655	40.64486637941981	19.849237723582743
21	13.34216518669635	34.55173406245503	33.763639287921244	18.34246146292738
22	8.527888058374883	17.415540111569165	46.7079478215232	27.348624008532756
23	21.74011495517764	25.621121955761726	37.71702233922782	14.921740749832816
24	18.145861021052543	28.419662583698035	30.760668060575792	22.67380833467363
25	10.542143177604903	42.89995174929952	28.724615475777306	17.833289597318277
26	10.600763546172534	29.409860072968602	37.24594313189371	22.74343324896515
27	23.200756774144395	21.720433748402222	29.634395130911766	25.44441434654161
28	9.1792725148773	28.608644493917872	37.301812364030376	24.910270627174455
29	16.90065434721881	17.30634115784751	37.01273141289902	28.780273082034658
30	9.29989926608145	35.259622628732025	32.57387859446556	22.866599510720967
31	14.985016887745168	22.95400099887415	26.366045050917187	35.694937062463495
32	21.98814048572372	31.11514140841255	26.222774330627345	20.673943775236385
33	17.06360627428407	24.591773678819635	27.532526897649262	30.812093149247033
34	17.19989334055683	19.40482337704113	33.74882547636985	29.646457806032185
35	21.62837649090432	19.432123115471544	27.69611370235243	31.243386691271702
36	13.969212667078631	26.464451084794256	26.394402918744124	33.17193332938299
37	11.731480619301973	21.932271253587057	34.639135550608216	31.697112576502757
38	15.036230350537105	28.530342918574824	21.055928487382864	35.3774982435052
39	16.51274411045178	23.520946729533662	33.16791243767618	26.79839672233838
40	17.195237571212107	23.961551810247773	31.144134153877413	27.69907646466271
41	12.247847764807462	21.355167480720883	29.651536827135516	36.74544792733614
42	19.567775304106387	19.56100327596861	35.99650394047387	24.874717479451128
43	19.951876275045922	21.35728373951394	23.78082330932085	34.91001667611929
44	16.825315534186043	17.77742036518162	32.01666765425411	33.380596446378235
45	18.14310988462157	17.81572464933592	29.774703088891336	34.26646237715117
46	26.639042435221317	21.88529030838123	24.814404103849053	26.661263152548397
47	11.788196354955854	22.16886898665064	40.40001523706331	25.642919421330195
48	17.72472552123454	20.112288691559513	28.73942928732869	33.42355649987726
49	17.117570873506978	15.939026351654492	37.66136473297046	29.282038041868063
50	16.42322636350554	15.848873727070337	33.0386090254205	34.68929088400362
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2765.0
1	2679.0
2	2593.0
3	9088.5
4	15584.0
5	11851.5
6	8119.0
7	7352.5
8	6586.0
9	6624.0
10	6662.0
11	6479.0
12	6296.0
13	6149.0
14	6002.0
15	5748.5
16	5495.0
17	5217.5
18	4940.0
19	4670.0
20	4400.0
21	4020.0
22	3640.0
23	3417.0
24	3194.0
25	3030.0
26	2866.0
27	2881.5
28	2897.0
29	2840.5
30	2784.0
31	3102.0
32	3420.0
33	3525.5
34	3631.0
35	4027.0
36	4423.0
37	4903.5
38	5384.0
39	6212.0
40	7040.0
41	9031.0
42	11022.0
43	15771.0
44	20520.0
45	32505.5
46	44491.0
47	53890.5
48	63290.0
49	60058.0
50	56826.0
51	51885.5
52	46945.0
53	40053.0
54	33161.0
55	27362.5
56	21564.0
57	18952.5
58	16341.0
59	14562.5
60	12784.0
61	11310.0
62	9836.0
63	8770.0
64	7704.0
65	6412.5
66	5121.0
67	4464.5
68	3808.0
69	3341.5
70	2875.0
71	2565.5
72	2256.0
73	1927.0
74	1598.0
75	1380.5
76	1163.0
77	971.5
78	780.0
79	722.0
80	664.0
81	549.5
82	435.0
83	368.5
84	302.0
85	243.5
86	185.0
87	136.0
88	87.0
89	60.0
90	33.0
91	21.5
92	10.0
93	8.0
94	6.0
95	5.0
96	4.0
97	2.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	472532.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	52.668511606013915
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.72171200874773	46.20172007032139
2	5.834280110127925	6.145656993860174
3	2.1432406213017035	3.386438806125277
4	1.1894575090904453	2.5058782648956206
5	0.6778242199620194	1.784999639795347
6	0.5116361277989506	1.6168268001021027
7	0.3611196725979469	1.3313744967169436
8	0.2687617900707348	1.1324226767674856
9	0.18923041354759976	0.8969835808928314
>10	0.9594550009536693	8.801037125259475
>50	0.0753657322257825	2.809274283266301
>100	0.05082805196617677	5.151913176669086
>500	0.006572592926660789	2.3347924031697342
>1k	0.008325284373770331	8.216198392571798
>5k	0.0021908643088869293	7.684483289586419
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	9388	1.9867437549203018	No Hit
GAATGATACGGCTGCCTCTTATACACATCTGACGCCCAAATGCTCGTATG	6668	1.411121363209264	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCCAAATGCTCGTATGCC	5877	1.243725292678591	No Hit
GAATCTGTCTCTTATACACATCTGACGCCCAAATGCTCGTATGCCGTCTT	5741	1.214944173093039	No Hit
GAATGATACCTGTCCCTTATACACATCTGACGCCCAAATGCTCGTATGCC	5624	1.1901839452142924	No Hit
GAATCTTTCTCTTACCCACATCTGACGCCCAAATGCTCGTATGCCGTCTT	4258	0.9011029940829405	No Hit
GAACTGTCCCTTATCCACATCTGACGCCCAAATGCTCGTATGCCGTCTTC	3846	0.8139131318090626	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCCAAATGCTCGTATG	2548	0.5392227404704866	No Hit
GAACTGTCCCTTATACACATCTGACGCCCAAATGCTCGTATGCCGTCTTC	2341	0.49541618345424226	No Hit
GAATCTGTCTCTTACCCACATCTGACGCCCAAATGCTCGTATGCCGTCTT	2303	0.4873744000406322	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2165	0.4581700286964693	No Hit
GAATGACTCTCTCTCCTACACATCTGACGCCCAAATGCTCGTATGCCGTC	1921	0.4065333141459203	No Hit
GCTGTCTCCTATACCCATCTGACGCCCAAATGCTCGTATGCCGTCTTCTG	1896	0.4012426671632821	No Hit
GAATGATACGGCTGCCTCTTATACACATCTGACGCCCCAATGCTCGTATG	1762	0.3728847993363412	No Hit
GAATGACTCTCTCTTATACACATCTGACGCCCAAATGCTCGTATGCCGTC	1632	0.34537343502662254	No Hit
GAATGATACCTGGCCCTTATACACATCTGACGCCCAAATGCTCGTATGCC	1449	0.3066458991137108	No Hit
GAATGATCCGTCTCCCATACACATCTGACGCCCAAATGCTCGTATGCCGT	1392	0.2945832239932957	No Hit
GAATGCTGCCTCTTCCACACATCTGACGCCCAAATGCTCGTATGCCGTCT	1366	0.28908095113135196	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCCCAATGCTCGTATGCC	1343	0.2842135559073248	No Hit
GAATCTGTCTCTTATACACATCTGACGCCCAAATGCTCGTATGCCGTCCT	1133	0.2397721212531638	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCCCAATGCTCGTATG	1092	0.23109546020163713	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCCAAATGC	1089	0.23046058256372057	No Hit
GAATCTTTCTCTTATACACATCTGACGCCCAAATGCTCGTATGCCGTCTT	1035	0.21903278508122204	No Hit
GCTGTCTCTTATACACATCTGACGCCCAAATGCTCGTATGCCGTCTTCTG	1031	0.21818628156399988	No Hit
GCTGTCTCCTATACACATCTGACGCCCAAATGCTCGTATGCCGTCTTCTG	892	0.18877028434053145	No Hit
GAATCTGTCTCTTATACACATCTGACGCCCCAATGCTCGTATGCCGTCTT	817	0.17289834339261678	No Hit
GAATCTTTCTCTTACCCCCATCTGACGCCCAAATGCTCGTATGCCGTCTT	767	0.16231704942734038	No Hit
CGTTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	764	0.1616821717894238	No Hit
GAATGATACGGCGACCGTCTCTTATACACATCTGACGCCCCAATGCTCGT	724	0.15321713661720265	No Hit
GAATGCTGCCTCTTATACACATCTGACGCCCAAATGCTCGTATGCCGTCT	714	0.15110087782414736	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCCCAAATGCTCG	673	0.14242421677262068	No Hit
GAACTGTCTCTTATACACATCTGACGCCCAAATGCTCGTATGCCGTCTTC	670	0.1417893391347041	No Hit
GAACTGTCCCTTATACACATCTGACGCCCAAATGCTCGTATGCCGTCCTC	670	0.1417893391347041	No Hit
GAATCTTTCTCTTACCCACATCTGACGCCCCAATGCTCGTATGCCGTCTT	663	0.1403079579795654	No Hit
GAATGATACGGCGACCGTCTCTTATACACATCTGACGCCCAAATGCTCGT	589	0.1246476429109563	No Hit
GAATGATCCGTCTCTTATACACATCTGACGCCCAAATGCTCGTATGCCGT	574	0.12147325472137335	No Hit
GAACTGTCCCTTATCCACATCTGACGCCCACATGCTCGTATGCCGTCTTC	547	0.11575935598012409	No Hit
GAATCTGTCTCTTATACACATCTGACGCCCCAATGCTCGTATGCCGTCCT	536	0.11343147130776328	No Hit
GAATCTGTCTCTTACACACATCTGACGCCCAAATGCTCGTATGCCGTCTT	517	0.10941057960095824	No Hit
GAATGATACCTGTCCCTTATACACATCTGACGCCCCAATGCTCGTATGCC	500	0.10581293965276427	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.1098338313595693	0.0
2	0.0	0.0	0.0	0.7375161893797668	0.0
3	0.0	0.0	0.0	1.3486917288141331	0.0
4	0.0	0.0	0.0	1.7643249557701912	0.0
5	0.0	0.0	0.0	5.612953196820532	0.0
6	0.0	0.0	0.0	5.829658097229394	0.0
7	0.0	0.0	0.0	6.012502856949371	0.0
8	0.0	0.0	0.0	6.222012477461844	0.0
9	0.0	0.0	0.0	6.506860911007085	0.0
10	0.0	0.0	0.0	9.698814048572372	0.0
11	0.0	0.0	0.0	9.893721483412763	0.0
12	0.0	0.0	0.0	11.76428263059433	0.0
13	0.0	0.0	0.0	11.867132807936818	0.0
14	0.0	0.0	0.0	11.962999331262221	0.0
15	0.0	0.0	0.0	12.227954932152743	0.0
16	0.0	0.0	0.0	12.676601796280464	0.0
17	0.0	0.0	0.0	12.840188600983637	0.0
18	0.0	0.0	0.0	12.961873481584316	0.0
19	0.0	0.0	0.0	13.865304360339618	0.0
20	0.0	0.0	0.0	14.056190903473205	0.0
21	0.0	0.0	0.0	14.138513370523054	0.0
22	0.0	0.0	0.0	14.346118358121778	0.0
23	0.0	0.0	0.0	14.46674510932593	0.0
24	0.0	0.0	0.0	14.817620817214495	0.0
25	0.0	0.0	0.0	14.91391059229851	0.0
26	0.0	0.0	0.0	15.258860775566523	0.0
27	0.0	0.0	0.0	16.060499606375863	0.0
28	0.0	0.0	0.0	16.211388858320706	0.0
29	0.0	0.0	0.0	16.4598376406254	0.0
30	0.0	0.0	0.0	16.581522521226077	0.0
31	0.0	0.0	0.0	16.72606299679175	0.0
32	0.0	0.0	0.0	16.97091413914825	0.0
33	0.0	0.0	0.0	17.04075067931907	0.0
34	0.0	0.0	0.0	17.26570898902085	0.0
35	0.0	0.0	0.0	17.345280319639727	0.0
36	0.0	0.0	0.0	17.405170443483193	0.0
37	0.0	0.0	0.0	17.481567385912488	0.0
38	0.0	0.0	0.0	17.559445709496924	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCACGAC	20	7.851768E-4	44.000004	25
GGTAATT	20	7.851768E-4	44.000004	8
ACCGCGT	40	8.294592E-9	44.000004	30
CGGTTTT	40	8.294592E-9	44.000004	1
TACGGAT	20	7.851768E-4	44.000004	7
GACCACG	20	7.851768E-4	44.000004	13
TGCGCGC	20	7.851768E-4	44.000004	15
TACGAGG	20	7.851768E-4	44.000004	2
CGGGCTT	20	7.851768E-4	44.000004	6
ACTACTA	20	7.851768E-4	44.000004	35
CGGGATC	20	7.851768E-4	44.000004	6
AGATTGG	20	7.851768E-4	44.000004	2
CTCGACC	20	7.851768E-4	44.000004	32
TCGACGG	20	7.851768E-4	44.000004	2
ATCGCTA	20	7.851768E-4	44.000004	13
GCATCGC	20	7.851768E-4	44.000004	22
TCGCCTA	25	4.438849E-5	44.0	30
CACGGGA	25	4.438849E-5	44.0	4
CGACGGT	25	4.438849E-5	44.0	28
CGCGCCT	25	4.438849E-5	44.0	17
>>END_MODULE