FastQCFastQC Report
Thu 26 May 2016
SRR1546145_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546145_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences547800
Sequences flagged as poor quality0
Sequence length50
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATCTGTCTCTTATACACATCTGACGCAATGACAGTCGTATGCCGTCTT390127.12157721796276No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAATGACAGTCGTATGCC296005.403431909456006No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT254624.648046732384082No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCAATGACAGTCGTATG208333.803030303030303No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA107021.9536327126688573No Hit
GAACTGTCTCTTATACACATCTGACGCAATGACAGTCGTATGCCGTCTTC104181.9017889740781309No Hit
GAATGACTGTCTCTTATACACATCTGACGCAATGACAGTCGTATGCCGTC61441.12157721796276No Hit
GCTGTCTCTTATACACATCTGACGCAATGACAGTCGTATGCCGTCTTCTG57241.044906900328587No Hit
GAATGATCTGTCTCTTATACACATCTGACGCAATGACAGTCGTATGCCGT55551.0140562248995983No Hit
GAATCTGTCTCTTATACACATCTGACGCAATGACAGTCGTATGCCGTCCT34920.6374589266155531No Hit
GAATGCTGTCTCTTATACACATCTGACGCAATGACAGTCGTATGCCGTCT29080.5308506754289887No Hit
GAATGATACCTGGCTCTTATACACATCTGACGCAATGACAGTCGTATGCC25090.45801387367652424No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCAATGACAGTCGT23480.4286235852500913No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCAATGACAG22180.4048922964585615No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA16600.30303030303030304No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCAATGACAGTCG14950.27290982110259215No Hit
CGCTGTCTCTTATACACATCTGACGCAATGACAGTCGTATGCCGTCTTCT9630.17579408543263964No Hit
GAATGATACTGTCTCTTATACACATCTGACGCAATGACAGTCGTATGCCG7540.1376414749908726No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCAATGACAGTCGTA7470.13636363636363638No Hit
CTGTCTCTTATACACATCTGACGCAATGACAGTCGTATGCCGTCTTCTGC7330.13380795910916393No Hit
GAATGATACGGCGACCACCGATGAATGATACGGCTATACACATCTGACGC7010.12796641109894122No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCAATGACAGTCGTAT6460.11792625045637094No Hit
TGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAAT6190.11299744432274553No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCTATA207.852868E-444.00000413
ATCCTAG408.29823E-944.00000414
CGAACGC207.852868E-444.00000434
TCACCGA207.852868E-444.00000415
TGGGTAT207.852868E-444.0000046
CCGTGTC207.852868E-444.00000431
TAAACCG207.852868E-444.00000427
GGATCCA207.852868E-444.0000048
TTCGTTG207.852868E-444.0000041
AGGGTCC207.852868E-444.0000046
TACGCCT408.29823E-944.00000429
TATCTCG207.852868E-444.00000427
CGAGGGA408.29823E-944.0000044
TAGGGTA650.044.0000045
CGGGATA207.852868E-444.0000046
ATTGGGT207.852868E-444.0000044
CAGCTCA207.852868E-444.00000442
ACCGTGT408.29823E-944.00000430
TATACTA408.29823E-944.00000444
GTGACGG408.29823E-944.0000042
Produced by FastQC (version 0.11.5)