FastQCFastQC Report
Thu 26 May 2016
SRR1546144_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546144_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences553101
Sequences flagged as poor quality0
Sequence length50
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATCTGTCTCTTATACACATCTGACGCAATGACAGTCGTATGCCGTCTT391977.086770770618747No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAATGACAGTCGTATGCC286445.178800978483134No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT261904.735120710322346No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCAATGACAGTCGTATG211593.825521920951146No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA104081.8817539653697968No Hit
GAACTGTCTCTTATACACATCTGACGCAATGACAGTCGTATGCCGTCTTC100451.8161239990526143No Hit
GAATGACTGTCTCTTATACACATCTGACGCAATGACAGTCGTATGCCGTC61011.1030535110223993No Hit
GCTGTCTCTTATACACATCTGACGCAATGACAGTCGTATGCCGTCTTCTG59081.0681593416030706No Hit
GAATGATCTGTCTCTTATACACATCTGACGCAATGACAGTCGTATGCCGT54130.9786639329887308No Hit
GAATGCTGTCTCTTATACACATCTGACGCAATGACAGTCGTATGCCGTCT29690.5367916528807578No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCAATGACAGTCGT26550.4800208280223684No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCAATGACAG25690.4644721307681599No Hit
GAATGATACCTGGCTCTTATACACATCTGACGCAATGACAGTCGTATGCC23580.42632358285376454No Hit
GAATCTGTCTCTTATACACATCTGACGCAATGACAGTCGTATGCCGTCCT21090.3813046803386723No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCAATGACAGTCG16130.291628472919051No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA16060.2903628812820805No Hit
GAATGATACGGCGACCACCGATGAATGATACGGCTATACACATCTGACGC9800.1771828291758648No Hit
CGCTGTCTCTTATACACATCTGACGCAATGACAGTCGTATGCCGTCTTCT8960.16199572953221925No Hit
CTGTCTCTTATACACATCTGACGCAATGACAGTCGTATGCCGTCTTCTGC8130.1469894286938552No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCAATGACAGTCGTA8050.14554303825160322No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCAATGACAGTCGTAT7630.13794948842978044No Hit
GAATGATACTGTCTCTTATACACATCTGACGCAATGACAGTCGTATGCCG6560.11860401626466051No Hit
TGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAAT5950.10757528914248934No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTATGC302.5253266E-644.08
ACCGGGC1000.044.030
GGGTACA207.852935E-444.07
CAACCGA302.5253266E-644.041
CCTACGC207.852935E-444.040
CGCTGTA207.852935E-444.034
CCTAACA302.5253266E-644.027
ACGGGTA207.852935E-444.05
TAGCCGT254.439836E-544.044
ACGGGCT454.783942E-1044.05
AGCGGGA502.7284841E-1144.04
TCGGACC207.852935E-444.015
TACTGTT454.783942E-1044.029
CTCGGAC207.852935E-444.014
TAACAAG207.852935E-444.037
TGTTCCC207.852935E-444.014
CGGTGTA302.5253266E-644.031
CACAGCG207.852935E-444.020
ACTCGCC207.852935E-444.023
TCATACG302.5253266E-644.026
Produced by FastQC (version 0.11.5)