##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546144_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	553101
Sequences flagged as poor quality	0
Sequence length	50
%GC	39
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.298489787579484	30.0	28.0	31.0	23.0	33.0
2	28.026430977344102	30.0	27.0	31.0	16.0	33.0
3	27.766200024950233	30.0	26.0	31.0	16.0	33.0
4	32.38812802725	35.0	32.0	35.0	28.0	35.0
5	33.32771410646519	35.0	33.0	35.0	28.0	37.0
6	33.5255477751803	35.0	33.0	35.0	29.0	37.0
7	33.61194610026017	35.0	33.0	35.0	30.0	37.0
8	33.0482027694761	35.0	32.0	35.0	28.0	37.0
9	33.75045064102216	35.0	32.0	39.0	27.0	39.0
10	34.430035382326196	35.0	33.0	39.0	28.0	39.0
11	34.49021426466414	37.0	33.0	39.0	27.0	39.0
12	33.93227819150571	35.0	32.0	39.0	27.0	39.0
13	33.41679006185127	35.0	31.0	39.0	25.0	39.0
14	35.516319804158734	37.0	34.0	40.0	27.0	41.0
15	34.91179368686732	37.0	32.0	40.0	27.0	41.0
16	35.240196636780624	36.0	33.0	40.0	27.0	41.0
17	34.40305116063793	36.0	32.0	39.0	25.0	41.0
18	34.04812321800178	36.0	32.0	39.0	26.0	40.0
19	32.90592314965983	35.0	31.0	37.0	24.0	40.0
20	32.45069526180571	35.0	30.0	37.0	23.0	39.0
21	33.45121415437687	35.0	31.0	38.0	25.0	40.0
22	33.915478366518954	35.0	32.0	38.0	26.0	40.0
23	34.023124167195505	35.0	32.0	38.0	26.0	40.0
24	34.327636362978915	35.0	33.0	39.0	25.0	40.0
25	34.610120032326826	35.0	33.0	39.0	27.0	40.0
26	33.71737892356008	35.0	33.0	38.0	24.0	40.0
27	33.6833905561552	35.0	33.0	39.0	24.0	40.0
28	33.62627982954289	35.0	33.0	39.0	23.0	40.0
29	33.495625572906214	35.0	33.0	38.0	24.0	40.0
30	33.450402367741155	35.0	33.0	39.0	22.0	40.0
31	32.97092032015853	35.0	32.0	38.0	22.0	40.0
32	32.65361660890145	35.0	32.0	39.0	16.0	40.0
33	32.11456135497857	35.0	31.0	38.0	15.0	40.0
34	31.18616491382225	35.0	30.0	38.0	9.0	40.0
35	30.89615639819852	35.0	29.0	38.0	8.0	40.0
36	30.91358721101571	35.0	28.0	38.0	10.0	40.0
37	30.668046161550965	35.0	27.0	38.0	8.0	40.0
38	30.76290406272995	35.0	27.0	39.0	10.0	40.0
39	30.467198576751805	35.0	27.0	38.0	8.0	40.0
40	30.07885359093547	35.0	25.0	38.0	8.0	40.0
41	29.444911508024756	34.0	24.0	37.0	8.0	40.0
42	29.335584278459088	34.0	23.0	38.0	8.0	40.0
43	29.57558746051806	35.0	24.0	38.0	7.0	40.0
44	30.037202970162774	35.0	25.0	38.0	8.0	40.0
45	29.97333398420903	35.0	24.0	38.0	8.0	40.0
46	29.40770130590977	34.0	23.0	38.0	7.0	40.0
47	29.502127097944136	34.0	24.0	37.0	8.0	40.0
48	29.605339711915185	35.0	24.0	38.0	7.0	40.0
49	29.44028305860955	34.0	24.0	38.0	7.0	40.0
50	29.384061862119214	34.0	23.0	38.0	7.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	2.0
11	0.0
12	3.0
13	5.0
14	28.0
15	126.0
16	434.0
17	1333.0
18	3058.0
19	4027.0
20	5227.0
21	6923.0
22	9040.0
23	11316.0
24	14420.0
25	17726.0
26	21211.0
27	22835.0
28	22147.0
29	22552.0
30	25883.0
31	32113.0
32	39010.0
33	47267.0
34	59030.0
35	61295.0
36	47486.0
37	42004.0
38	31517.0
39	5083.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	53.26133924907024	13.797480026251987	10.578899694630818	22.362281030046955
2	27.141155051247424	54.01563186470464	11.144619156356615	7.698593927691326
3	12.208258527827647	52.97459234389379	27.468762486417493	7.348386641861071
4	12.257074205253652	14.866181764270902	63.47719494269582	9.399549087779627
5	38.83214819716471	16.35361353532176	28.69710956949996	16.117128698013563
6	10.954780410811045	47.75493083541704	33.4069184470829	7.883370306689014
7	33.116736364606105	9.62952516809769	51.83248629093059	5.421252176365619
8	25.36155241086167	35.30693309178613	30.187072523824764	9.144441973527439
9	24.793663363472493	9.55684404837453	26.410185481494334	39.239307106658636
10	35.59729597306821	15.018775955928485	33.55463107099788	15.829297000005424
11	33.148556954335646	15.440760367455491	33.56764858497815	17.84303409323071
12	18.096514018235368	13.833458988503002	39.16481799888266	28.905208994378967
13	24.23662224440021	20.82784156962291	45.75077607887167	9.184760107105213
14	11.564433982220246	42.511042287032566	29.661671195676735	16.26285253507045
15	7.0386782884138706	19.45160106382017	50.53073489290383	22.978985754862133
16	7.445114002686671	23.511799834026696	32.15199393962405	36.89109222366259
17	7.7513871788335225	31.185624325394457	37.834500389621425	23.228488106150593
18	8.522313284553816	25.412537673951054	35.6403260887252	30.424822952769926
19	9.91753766491111	26.108793873090086	29.81426538733432	34.15940307466448
20	20.71429991990613	25.04985527055637	39.72764467972396	14.508200129813542
21	12.666221901605674	38.492608040846065	34.70378827736707	14.137381780181196
22	9.508932364974934	18.715388328713924	43.352841524423205	28.42283778188794
23	20.99634605614526	29.294288023344745	36.61555484441359	13.09381107609641
24	18.60148508138658	29.856029911354348	30.536918212044455	21.005566795214616
25	10.482172333805218	45.712446732151996	28.748275631394627	15.05710530264816
26	11.57220833084735	20.376025355224453	43.71823590989711	24.33353040403109
27	27.103910497359436	25.075709499711625	28.306764948897218	19.51361505403172
28	9.24008454152135	31.274758136398233	40.12702924059078	19.358128081489635
29	17.74992270851074	28.201178446612822	38.015299194902916	16.033599649973514
30	9.958036597294164	44.693103067974924	33.74862818906493	11.60023214566598
31	18.185647829239144	19.300995659020685	33.297535169887595	29.21582134185257
32	33.83505001798948	28.376914885346437	26.613945735046585	11.174089361617497
33	22.03865116859308	26.754426406750305	25.60798118246035	25.598941242196272
34	16.579069645507783	27.11819360297667	27.77847083986469	28.524265911650854
35	19.830916957300744	34.2872278299985	26.40584631016758	19.476008902533174
36	23.235539259556575	30.80576603549804	30.58898826796553	15.369706436979863
37	17.76854498545474	27.199191467742782	35.32862894841991	19.703634598382575
38	14.830564399630447	30.7260337623689	26.39644477229294	28.046957065707712
39	24.988022079150102	22.938486822479078	26.74520566768095	25.328285430689874
40	12.427025082218258	33.23660597250773	32.98800761524568	21.348361330028332
41	18.10645795252585	26.118557008575287	25.663305616876485	30.11167942202238
42	15.568042726373665	26.22052753475405	38.82238506167951	19.389044677192775
43	26.374025720438038	23.566039475611145	24.842117443287933	25.21781736066288
44	17.690801499183692	21.946443777899518	31.64196050992495	28.72079421299184
45	16.311125816080608	20.977362181590703	31.665283555806262	31.046228446522427
46	31.176945982740946	26.465690714715755	24.58013997443505	17.77722332810825
47	13.002146081818692	24.98042852932828	42.28450138401485	19.732924004838175
48	19.133756764135303	23.813010643625667	27.265002232865243	29.788230359373784
49	16.41942430044422	20.10139197000186	40.15469145779885	23.32449227175507
50	17.891488173046152	20.374759763587484	35.37455184496141	26.359200218404954
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	11876.0
1	8511.5
2	5147.0
3	19265.0
4	33383.0
5	20815.0
6	8247.0
7	7794.5
8	7342.0
9	7415.5
10	7489.0
11	7233.0
12	6977.0
13	6633.0
14	6289.0
15	5755.0
16	5221.0
17	4774.5
18	4328.0
19	3917.0
20	3506.0
21	3199.0
22	2892.0
23	2616.5
24	2341.0
25	2392.5
26	2444.0
27	2544.0
28	2644.0
29	2645.5
30	2647.0
31	3012.5
32	3378.0
33	4003.0
34	4628.0
35	5623.5
36	6619.0
37	7489.5
38	8360.0
39	10172.5
40	11985.0
41	16715.0
42	21445.0
43	74234.0
44	127023.0
45	97309.0
46	67595.0
47	57734.5
48	47874.0
49	40754.0
50	33634.0
51	30441.5
52	27249.0
53	23989.5
54	20730.0
55	18352.5
56	15975.0
57	14461.0
58	12947.0
59	11457.0
60	9967.0
61	8550.5
62	7134.0
63	5943.5
64	4753.0
65	3855.0
66	2957.0
67	2484.0
68	2011.0
69	1729.0
70	1447.0
71	1333.0
72	1219.0
73	959.0
74	699.0
75	521.0
76	343.0
77	239.5
78	136.0
79	125.5
80	115.0
81	78.0
82	41.0
83	34.5
84	28.0
85	19.0
86	10.0
87	9.5
88	9.0
89	9.0
90	9.0
91	6.5
92	4.0
93	3.5
94	3.0
95	2.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	553101.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	29.476995015381586
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.30514271302694	23.3767729644602
2	6.733264457767005	3.969528057176879
3	3.083380264957034	2.726663541019933
4	2.0720792114836493	2.443146743535173
5	1.4810285074726057	2.182813496620401
6	1.1936088374414642	2.111040105094647
7	0.8881415323166979	1.8325820464736866
8	0.7782951496618887	1.8353441797663326
9	0.628690529003601	1.6678716830691083
>10	3.6116608928021496	18.544124751181727
>50	0.11296790805555168	2.3058124896002066
>100	0.09639109546044355	5.247573891252459
>500	0.0055256041983693755	1.1819528584552979
>1k	0.004297692154287292	2.8737145061111122
>5k	0.0018418680661231252	3.1529601439302097
>10k+	0.0036837361322462504	24.548098542252635
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATCTGTCTCTTATACACATCTGACGCAATGACAGTCGTATGCCGTCTT	39197	7.086770770618747	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAATGACAGTCGTATGCC	28644	5.178800978483134	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	26190	4.735120710322346	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCAATGACAGTCGTATG	21159	3.825521920951146	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	10408	1.8817539653697968	No Hit
GAACTGTCTCTTATACACATCTGACGCAATGACAGTCGTATGCCGTCTTC	10045	1.8161239990526143	No Hit
GAATGACTGTCTCTTATACACATCTGACGCAATGACAGTCGTATGCCGTC	6101	1.1030535110223993	No Hit
GCTGTCTCTTATACACATCTGACGCAATGACAGTCGTATGCCGTCTTCTG	5908	1.0681593416030706	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCAATGACAGTCGTATGCCGT	5413	0.9786639329887308	No Hit
GAATGCTGTCTCTTATACACATCTGACGCAATGACAGTCGTATGCCGTCT	2969	0.5367916528807578	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCAATGACAGTCGT	2655	0.4800208280223684	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCAATGACAG	2569	0.4644721307681599	No Hit
GAATGATACCTGGCTCTTATACACATCTGACGCAATGACAGTCGTATGCC	2358	0.42632358285376454	No Hit
GAATCTGTCTCTTATACACATCTGACGCAATGACAGTCGTATGCCGTCCT	2109	0.3813046803386723	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCAATGACAGTCG	1613	0.291628472919051	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1606	0.2903628812820805	No Hit
GAATGATACGGCGACCACCGATGAATGATACGGCTATACACATCTGACGC	980	0.1771828291758648	No Hit
CGCTGTCTCTTATACACATCTGACGCAATGACAGTCGTATGCCGTCTTCT	896	0.16199572953221925	No Hit
CTGTCTCTTATACACATCTGACGCAATGACAGTCGTATGCCGTCTTCTGC	813	0.1469894286938552	No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCAATGACAGTCGTA	805	0.14554303825160322	No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCAATGACAGTCGTAT	763	0.13794948842978044	No Hit
GAATGATACTGTCTCTTATACACATCTGACGCAATGACAGTCGTATGCCG	656	0.11860401626466051	No Hit
TGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAAT	595	0.10757528914248934	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.17971401244980573	0.0
2	0.0	0.0	0.0	1.6214036857644445	0.0
3	0.0	0.0	0.0	1.9247840810267927	0.0
4	0.0	0.0	0.0	4.179706780497594	0.0
5	0.0	0.0	0.0	12.79748183424004	0.0
6	0.0	0.0	0.0	13.750472336878797	0.0
7	0.0	0.0	0.0	15.257068781289494	0.0
8	0.0	0.0	0.0	16.659344315052767	0.0
9	0.0	0.0	0.0	16.92349136956903	0.0
10	0.0	0.0	0.0	22.65246311252375	0.0
11	1.807988052814947E-4	0.0	0.0	22.860743336208035	0.0
12	1.807988052814947E-4	0.0	0.0	27.35992160563803	0.0
13	1.807988052814947E-4	0.0	0.0	27.642690937098287	0.0
14	1.807988052814947E-4	0.0	0.0	27.92094029842651	0.0
15	1.807988052814947E-4	0.0	0.0	28.5951390433212	0.0
16	1.807988052814947E-4	0.0	0.0	29.04984803860416	0.0
17	1.807988052814947E-4	0.0	0.0	29.20461181592512	0.0
18	1.807988052814947E-4	0.0	0.0	29.321046246526404	0.0
19	1.807988052814947E-4	0.0	0.0	30.091068358220287	0.0
20	1.807988052814947E-4	0.0	0.0	30.218169918333178	0.0
21	1.807988052814947E-4	0.0	0.0	30.281630298986983	0.0
22	1.807988052814947E-4	0.0	0.0	30.500216054572313	0.0
23	1.807988052814947E-4	0.0	0.0	30.5765131504011	0.0
24	1.807988052814947E-4	0.0	0.0	30.83523624075892	0.0
25	3.615976105629894E-4	0.0	0.0	30.914606916277496	0.0
26	5.423964158444841E-4	0.0	0.0	31.24420313830566	0.0
27	7.231952211259788E-4	0.0	0.0	31.645214888420018	0.0
28	7.231952211259788E-4	0.0	0.0	31.729286332875912	0.0
29	7.231952211259788E-4	0.0	0.0	31.834692036355023	0.0
30	7.231952211259788E-4	0.0	0.0	31.901587594309177	0.0
31	9.039940264074735E-4	0.0	0.0	31.998134156329495	0.0
32	9.039940264074735E-4	0.0	0.0	32.13590284595399	0.0
33	9.039940264074735E-4	0.0	0.0	32.19719364094442	0.0
34	9.039940264074735E-4	0.0	0.0	32.33478153176364	0.0
35	9.039940264074735E-4	0.0	0.0	32.406739456265676	0.0
36	9.039940264074735E-4	0.0	0.0	32.45953270740787	0.0
37	0.0010847928316889681	0.0	0.0	32.518111520319074	0.0
38	0.0010847928316889681	0.0	0.0	32.57072397265599	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACTATGC	30	2.5253266E-6	44.0	8
ACCGGGC	100	0.0	44.0	30
GGGTACA	20	7.852935E-4	44.0	7
CAACCGA	30	2.5253266E-6	44.0	41
CCTACGC	20	7.852935E-4	44.0	40
CGCTGTA	20	7.852935E-4	44.0	34
CCTAACA	30	2.5253266E-6	44.0	27
ACGGGTA	20	7.852935E-4	44.0	5
TAGCCGT	25	4.439836E-5	44.0	44
ACGGGCT	45	4.783942E-10	44.0	5
AGCGGGA	50	2.7284841E-11	44.0	4
TCGGACC	20	7.852935E-4	44.0	15
TACTGTT	45	4.783942E-10	44.0	29
CTCGGAC	20	7.852935E-4	44.0	14
TAACAAG	20	7.852935E-4	44.0	37
TGTTCCC	20	7.852935E-4	44.0	14
CGGTGTA	30	2.5253266E-6	44.0	31
CACAGCG	20	7.852935E-4	44.0	20
ACTCGCC	20	7.852935E-4	44.0	23
TCATACG	30	2.5253266E-6	44.0	26
>>END_MODULE