FastQCFastQC Report
Thu 26 May 2016
SRR1546143_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546143_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences550763
Sequences flagged as poor quality0
Sequence length50
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATCTGTCTCTTATACACATCTGACGCAATGACAGTCGTATGCCGTCTT397997.226157167420469No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAATGACAGTCGTATGCC285405.181902197496927No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT255454.638111129469482No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCAATGACAGTCGTATG207103.760238069732353No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA104031.8888342172586032No Hit
GAACTGTCTCTTATACACATCTGACGCAATGACAGTCGTATGCCGTCTTC99811.8122132387251868No Hit
GAATGACTGTCTCTTATACACATCTGACGCAATGACAGTCGTATGCCGTC61951.1248032275225461No Hit
GCTGTCTCTTATACACATCTGACGCAATGACAGTCGTATGCCGTCTTCTG59501.0803194840612023No Hit
GAATGATCTGTCTCTTATACACATCTGACGCAATGACAGTCGTATGCCGT55591.0093270608228948No Hit
GAATGCTGTCTCTTATACACATCTGACGCAATGACAGTCGTATGCCGTCT29700.5392519105313901No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCAATGACAGTCGT26700.48478202057872444No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCAATGACAG26340.47824563378440454No Hit
GAATGATACCTGGCTCTTATACACATCTGACGCAATGACAGTCGTATGCC24520.4452005672131207No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA17500.31774102472388305No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCAATGACAGTCG16060.29159547754660353No Hit
GAATCTGTCTCTTATACACATCTGACGCAATGACAGTCGTATGCCGTCCT13500.2451145047869955No Hit
GAATGATACGGCGACCACCGATGAATGATACGGCTATACACATCTGACGC9700.17611931084695231No Hit
CGCTGTCTCTTATACACATCTGACGCAATGACAGTCGTATGCCGTCTTCT9430.1712170207512124No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCAATGACAGTCGTA8690.1577811145628882No Hit
CTGTCTCTTATACACATCTGACGCAATGACAGTCGTATGCCGTCTTCTGC7780.14125858127724628No Hit
GAATGATACTGTCTCTTATACACATCTGACGCAATGACAGTCGTATGCCG7250.1316355673856087No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCAATGACAGTCGTAT6980.1267332772898688No Hit
GAACTGTCTCTTATACACATCTGACGCAATGACAGTCGTATGCCGTCCTC6680.12128628829460222No Hit
TGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAAT6160.11184484070280683No Hit
GAATGATACGGCGACCACCCGTCTCTTATACACATCTGACGCAATGACAG5580.10131399531195814No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCCTA650.044.00000430
ATCCTAG302.5253066E-644.014
GTGCAAG502.7284841E-1144.01
ACTATGC207.852908E-444.08
TAATACG254.4398104E-544.04
GGTGTTC254.4398104E-544.09
TCACCGA207.852908E-444.015
AGATAGA207.852908E-444.023
CGTGCGG254.4398104E-544.02
TAGAATG207.852908E-444.01
CCGTGTA454.783942E-1044.031
GATCCAC700.044.035
TAAACCG254.4398104E-544.027
GCGCCTA254.4398104E-544.033
TATATCG408.29823E-944.027
AGGGTCC207.852908E-444.06
TGCGGGC351.4442412E-744.04
CCTAACA254.4398104E-544.027
CGTTCGA302.5253066E-644.014
ATCGTGT351.4442412E-744.030
Produced by FastQC (version 0.11.5)