##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546143_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	550763
Sequences flagged as poor quality	0
Sequence length	50
%GC	40
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.1857296150976	30.0	28.0	31.0	16.0	33.0
2	27.946138357151806	30.0	27.0	31.0	16.0	33.0
3	27.740354744236633	30.0	26.0	31.0	16.0	33.0
4	32.379500801615215	35.0	32.0	35.0	28.0	35.0
5	33.325711785286956	35.0	33.0	35.0	28.0	37.0
6	33.517567447341236	35.0	33.0	35.0	29.0	37.0
7	33.54806150739974	35.0	33.0	35.0	30.0	37.0
8	33.01709446713014	35.0	32.0	35.0	28.0	37.0
9	33.651519800712826	35.0	32.0	39.0	27.0	39.0
10	34.41136024024853	35.0	32.0	39.0	28.0	39.0
11	34.477092687780406	37.0	33.0	39.0	27.0	39.0
12	34.051517621917235	35.0	32.0	39.0	27.0	39.0
13	33.45065845018638	35.0	31.0	39.0	25.0	39.0
14	35.54074620117909	37.0	34.0	40.0	29.0	41.0
15	34.84244402764892	37.0	32.0	40.0	27.0	41.0
16	35.153993641548176	36.0	33.0	40.0	27.0	41.0
17	34.29987853214541	36.0	32.0	39.0	25.0	41.0
18	33.95609182170916	36.0	32.0	39.0	25.0	39.0
19	32.755366645907586	35.0	30.0	37.0	23.0	39.0
20	32.39696203267104	35.0	30.0	37.0	23.0	39.0
21	33.59342584741531	35.0	32.0	38.0	25.0	40.0
22	33.89770736233189	35.0	32.0	38.0	26.0	40.0
23	33.97081503296336	35.0	32.0	38.0	25.0	40.0
24	34.29661397007424	35.0	33.0	39.0	25.0	40.0
25	34.63749925103901	35.0	33.0	39.0	27.0	40.0
26	33.67254154690856	35.0	33.0	38.0	24.0	40.0
27	33.63090294736575	35.0	33.0	39.0	24.0	40.0
28	33.553898863939665	35.0	33.0	39.0	23.0	40.0
29	33.41636965446118	35.0	32.0	38.0	23.0	40.0
30	33.351387075747645	35.0	33.0	39.0	22.0	40.0
31	32.88468179598121	35.0	31.0	38.0	21.0	40.0
32	32.55703996092693	35.0	31.0	39.0	16.0	40.0
33	32.002298629356005	35.0	31.0	38.0	15.0	40.0
34	31.075691359078224	35.0	29.0	38.0	9.0	40.0
35	30.75510700609881	35.0	28.0	38.0	8.0	40.0
36	30.835214057589198	35.0	27.0	38.0	10.0	40.0
37	30.631315102866388	35.0	27.0	38.0	8.0	40.0
38	30.677498306894254	35.0	27.0	39.0	9.0	40.0
39	30.376321575704978	35.0	26.0	38.0	8.0	40.0
40	30.036500273257282	35.0	25.0	38.0	8.0	40.0
41	29.46479883361809	34.0	24.0	37.0	8.0	40.0
42	29.329588225788587	34.0	23.0	38.0	8.0	40.0
43	29.54837561709846	35.0	24.0	38.0	7.0	40.0
44	30.030383304615597	35.0	25.0	38.0	8.0	40.0
45	29.992165414161807	35.0	24.0	38.0	8.0	40.0
46	29.459705898907515	35.0	23.0	38.0	7.0	40.0
47	29.567440078581896	34.0	24.0	37.0	8.0	40.0
48	29.617657685792256	35.0	24.0	38.0	7.0	40.0
49	29.527635298667484	35.0	24.0	38.0	7.0	40.0
50	29.389323538436678	34.0	23.0	38.0	7.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	2.0
12	2.0
13	10.0
14	19.0
15	110.0
16	429.0
17	1304.0
18	2846.0
19	4165.0
20	5619.0
21	7261.0
22	9231.0
23	11702.0
24	14330.0
25	17865.0
26	21348.0
27	22907.0
28	21941.0
29	22615.0
30	25993.0
31	31373.0
32	38711.0
33	46766.0
34	58489.0
35	60553.0
36	46601.0
37	41354.0
38	31548.0
39	5669.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	53.61198918591118	13.681928524610404	10.570789976813984	22.135292312664433
2	26.8133480280992	54.38872981663619	11.14090815831855	7.657013996946055
3	12.219048846781646	53.31785178016679	27.142709296012985	7.3203900770385815
4	12.273518736734312	14.794203677443837	63.49228252442521	9.439995061396644
5	39.17093196166046	16.309374449627153	28.521342210714955	15.998351377997434
6	10.965515112670968	48.07766680042051	33.152735387090274	7.8040826998182515
7	32.85442195644951	9.556742192195191	52.12695841950167	5.461877431853628
8	25.23408435207158	35.693211054482596	29.83243246187562	9.240272131570205
9	24.688477621045713	9.545121949005289	26.220534059114353	39.545866370834645
10	35.95539279145476	15.033689626935724	33.26276456479466	15.748153016814856
11	33.47319990631179	15.441124403781664	33.402025916773646	17.68364977313291
12	18.059310447506462	13.953188576574679	38.949784208452634	29.037716767466225
13	24.667415930264017	20.826562423401718	45.37959158476513	9.126430061569133
14	11.467364365434861	42.75831891394302	29.450235400707747	16.324081319914374
15	7.05112725437257	19.382384074456706	50.10993839455446	23.45655027661626
16	7.482528782797683	23.272260482276405	31.826575133042706	37.4186356018832
17	7.685338339721441	31.78172825698168	37.604377926621794	22.928555476675086
18	8.552862120367562	25.136220116456627	35.405065336632994	30.90585242654281
19	9.847611404542425	26.0456857123663	29.562261807710392	34.54444107538088
20	21.19659454248016	24.997321897077327	39.611230238777836	14.194853321664672
21	12.687853032974255	38.84974117723958	34.348531037851124	14.113874751935043
22	9.555652794396138	18.70405237824618	43.427390728861596	28.312904098496087
23	21.53285532978795	29.15192197006698	36.1890686193517	13.126154080793373
24	18.551536686378714	30.31666978355481	30.31812231395355	20.81367121611292
25	10.45513224381449	45.90940204770473	28.475769069454554	15.159696639026224
26	11.673078983156095	20.324894736937665	43.8531637019916	24.148862577914638
27	27.490590326510677	24.942488874524976	28.108460444873746	19.458460354090597
28	9.223386465684877	31.618863286023206	39.77863436723237	19.379115881059548
29	17.75682099196932	28.153670453534463	38.026156441155265	16.063352113340947
30	9.999582397510363	44.95853933543103	33.50969473258007	11.532183534478532
31	18.085274428383897	19.146529450961665	32.97189535244742	29.79630076820701
32	34.08998062687581	28.250626857650207	26.378678306276925	11.28071420919706
33	22.410546823225236	26.604365216980806	25.404938240223107	25.580149719570848
34	16.669601988514117	27.133449414721035	27.59154118922295	28.6054074075419
35	19.99063117892814	34.27009439631929	26.26320213957728	19.476072285175295
36	23.230137100713012	31.082516436289293	30.35153051312452	15.335815949873178
37	17.715968574504824	27.239121001229204	35.23548241258037	19.809428011685608
38	14.906956349645856	30.703587568518582	26.13192970479135	28.25752637704421
39	24.83736198691633	23.035861159881836	26.58675328589611	25.54002356730572
40	12.412780088713294	33.167805389977175	32.72569144986137	21.693723071448154
41	18.256673015434952	25.990307990914424	25.423821135406698	30.32919785824393
42	15.684423245570237	26.30532552114067	38.482977251558296	19.5272739817308
43	26.206371887726664	23.652097181546328	24.76019630948339	25.381334621243617
44	17.629543015779927	21.988405176092076	31.53134832949926	28.850703478628738
45	16.388718922658203	21.054064997104017	31.688766311462462	30.86844976877532
46	31.387003121124696	26.503595920568372	24.565194103452846	17.54420685485408
47	13.005594057698138	25.34284256567707	41.99773768390397	19.653825692720826
48	19.25002224187173	23.771386240542665	27.00744240263053	29.97114911495507
49	16.439739052913865	20.22703050132271	40.27812325809831	23.05510718766511
50	17.976153082178723	20.48140488740166	35.184643848624546	26.357798181795072
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	11868.0
1	8475.5
2	5083.0
3	18813.5
4	32544.0
5	20350.0
6	8156.0
7	7682.0
8	7208.0
9	7374.0
10	7540.0
11	7173.0
12	6806.0
13	6436.0
14	6066.0
15	5509.5
16	4953.0
17	4652.5
18	4352.0
19	3905.5
20	3459.0
21	3091.5
22	2724.0
23	2466.5
24	2209.0
25	2324.0
26	2439.0
27	2541.5
28	2644.0
29	2709.0
30	2774.0
31	2976.0
32	3178.0
33	3911.5
34	4645.0
35	5703.5
36	6762.0
37	7622.0
38	8482.0
39	10112.5
40	11743.0
41	16681.5
42	21620.0
43	74241.5
44	126863.0
45	96738.0
46	66613.0
47	57257.0
48	47901.0
49	40907.5
50	33914.0
51	30456.5
52	26999.0
53	23969.0
54	20939.0
55	18548.5
56	16158.0
57	14684.5
58	13211.0
59	11758.5
60	10306.0
61	8672.0
62	7038.0
63	5786.5
64	4535.0
65	3711.5
66	2888.0
67	2473.0
68	2058.0
69	1741.0
70	1424.0
71	1325.0
72	1226.0
73	977.0
74	728.0
75	538.0
76	348.0
77	250.0
78	152.0
79	134.0
80	116.0
81	76.5
82	37.0
83	28.0
84	19.0
85	13.5
86	8.0
87	9.5
88	11.0
89	8.0
90	5.0
91	5.5
92	6.0
93	3.5
94	1.0
95	2.0
96	3.0
97	2.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	550763.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	29.72193939450518
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.3583172984859	23.586830971955102
2	6.73887004014972	4.005845738415534
3	3.0768471777214237	2.743495960271714
4	2.049581574708415	2.4367015739031195
5	1.4838993695520617	2.205218356468541
6	1.1791569122763836	2.102809816997432
7	0.9430292818153678	1.9620061412952077
8	0.7647675562280575	1.8184299965675326
9	0.6132557640788481	1.640443558795424
>10	3.5840181131141637	18.556778698158915
>50	0.10169270257960271	2.1425393060872655
>100	0.0901228741424024	4.949124626200057
>500	0.006698321726800178	1.423110877155277
>1k	0.004262568371600113	2.7930111988121884
>5k	0.0024357533552000652	5.010660642762794
>10k+	0.0030446916940000813	22.62299253615391
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATCTGTCTCTTATACACATCTGACGCAATGACAGTCGTATGCCGTCTT	39799	7.226157167420469	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAATGACAGTCGTATGCC	28540	5.181902197496927	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	25545	4.638111129469482	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCAATGACAGTCGTATG	20710	3.760238069732353	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	10403	1.8888342172586032	No Hit
GAACTGTCTCTTATACACATCTGACGCAATGACAGTCGTATGCCGTCTTC	9981	1.8122132387251868	No Hit
GAATGACTGTCTCTTATACACATCTGACGCAATGACAGTCGTATGCCGTC	6195	1.1248032275225461	No Hit
GCTGTCTCTTATACACATCTGACGCAATGACAGTCGTATGCCGTCTTCTG	5950	1.0803194840612023	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCAATGACAGTCGTATGCCGT	5559	1.0093270608228948	No Hit
GAATGCTGTCTCTTATACACATCTGACGCAATGACAGTCGTATGCCGTCT	2970	0.5392519105313901	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCAATGACAGTCGT	2670	0.48478202057872444	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCAATGACAG	2634	0.47824563378440454	No Hit
GAATGATACCTGGCTCTTATACACATCTGACGCAATGACAGTCGTATGCC	2452	0.4452005672131207	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1750	0.31774102472388305	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCAATGACAGTCG	1606	0.29159547754660353	No Hit
GAATCTGTCTCTTATACACATCTGACGCAATGACAGTCGTATGCCGTCCT	1350	0.2451145047869955	No Hit
GAATGATACGGCGACCACCGATGAATGATACGGCTATACACATCTGACGC	970	0.17611931084695231	No Hit
CGCTGTCTCTTATACACATCTGACGCAATGACAGTCGTATGCCGTCTTCT	943	0.1712170207512124	No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCAATGACAGTCGTA	869	0.1577811145628882	No Hit
CTGTCTCTTATACACATCTGACGCAATGACAGTCGTATGCCGTCTTCTGC	778	0.14125858127724628	No Hit
GAATGATACTGTCTCTTATACACATCTGACGCAATGACAGTCGTATGCCG	725	0.1316355673856087	No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCAATGACAGTCGTAT	698	0.1267332772898688	No Hit
GAACTGTCTCTTATACACATCTGACGCAATGACAGTCGTATGCCGTCCTC	668	0.12128628829460222	No Hit
TGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAAT	616	0.11184484070280683	No Hit
GAATGATACGGCGACCACCCGTCTCTTATACACATCTGACGCAATGACAG	558	0.10131399531195814	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.1768455760463212	0.0
2	1.8156629984221887E-4	0.0	0.0	1.6420856157730277	0.0
3	1.8156629984221887E-4	0.0	0.0	1.9629132675942285	0.0
4	1.8156629984221887E-4	0.0	0.0	4.273162866786621	0.0
5	1.8156629984221887E-4	0.0	0.0	12.844363183438249	0.0
6	1.8156629984221887E-4	0.0	0.0	13.795407462011791	0.0
7	1.8156629984221887E-4	0.0	0.0	15.309488836396055	0.0
8	1.8156629984221887E-4	0.0	0.0	16.733876458658262	0.0
9	1.8156629984221887E-4	0.0	0.0	17.00477337802285	0.0
10	3.6313259968443775E-4	0.0	0.0	22.750802069129552	0.0
11	3.6313259968443775E-4	0.0	0.0	22.962326808445738	0.0
12	3.6313259968443775E-4	0.0	0.0	27.380016449906766	0.0
13	7.262651993688755E-4	0.0	0.0	27.644195416177194	0.0
14	7.262651993688755E-4	0.0	0.0	27.9392406534208	0.0
15	7.262651993688755E-4	0.0	0.0	28.62429030272549	0.0
16	7.262651993688755E-4	0.0	0.0	29.070398701437824	0.0
17	7.262651993688755E-4	0.0	0.0	29.235079335394715	0.0
18	7.262651993688755E-4	0.0	0.0	29.334759234008093	0.0
19	7.262651993688755E-4	0.0	0.0	30.124935770921432	0.0
20	9.078314992110944E-4	0.0	0.0	30.260021098004042	0.0
21	0.0010893977990533133	0.0	0.0	30.32465870074787	0.0
22	0.0010893977990533133	0.0	0.0	30.553250672249224	0.0
23	0.0010893977990533133	0.0	0.0	30.63150574748122	0.0
24	0.0010893977990533133	0.0	0.0	30.887151097659064	0.0
25	0.0010893977990533133	0.0	0.0	30.968674366288223	0.0
26	0.0010893977990533133	0.0	0.0	31.29803563420201	0.0
27	0.0010893977990533133	0.0	0.0	31.695665830856466	0.0
28	0.0010893977990533133	0.0	0.0	31.78372548627994	0.0
29	0.0010893977990533133	0.0	0.0	31.897567556281015	0.0
30	0.0010893977990533133	0.0	0.0	31.969286244718692	0.0
31	0.0010893977990533133	0.0	0.0	32.062429756537746	0.0
32	0.0010893977990533133	0.0	0.0	32.208772194210574	0.0
33	0.0012709640988955322	0.0	0.0	32.27086786875662	0.0
34	0.001452530398737751	0.0	0.0	32.40214030354254	0.0
35	0.001452530398737751	0.0	0.0	32.46786730408542	0.0
36	0.001452530398737751	0.0	0.0	32.52596852003494	0.0
37	0.001452530398737751	0.0	0.0	32.5757176861917	0.0
38	0.001815662998422189	0.0	0.0	32.63835805963727	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGCCTA	65	0.0	44.000004	30
ATCCTAG	30	2.5253066E-6	44.0	14
GTGCAAG	50	2.7284841E-11	44.0	1
ACTATGC	20	7.852908E-4	44.0	8
TAATACG	25	4.4398104E-5	44.0	4
GGTGTTC	25	4.4398104E-5	44.0	9
TCACCGA	20	7.852908E-4	44.0	15
AGATAGA	20	7.852908E-4	44.0	23
CGTGCGG	25	4.4398104E-5	44.0	2
TAGAATG	20	7.852908E-4	44.0	1
CCGTGTA	45	4.783942E-10	44.0	31
GATCCAC	70	0.0	44.0	35
TAAACCG	25	4.4398104E-5	44.0	27
GCGCCTA	25	4.4398104E-5	44.0	33
TATATCG	40	8.29823E-9	44.0	27
AGGGTCC	20	7.852908E-4	44.0	6
TGCGGGC	35	1.4442412E-7	44.0	4
CCTAACA	25	4.4398104E-5	44.0	27
CGTTCGA	30	2.5253066E-6	44.0	14
ATCGTGT	35	1.4442412E-7	44.0	30
>>END_MODULE