FastQCFastQC Report
Thu 26 May 2016
SRR1546142_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546142_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences463560
Sequences flagged as poor quality0
Sequence length50
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATGATACGGCTGCCTCTTATACACATCTGACGCAATGACAGTCGTATG145893.1471654154801962No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT116422.5114332556734835No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAATGACAGTCGTATGCC85471.8437742687030805No Hit
GAATCTGTCTCTTATACACATCTGACGCAATGACAGTCGTATGCCGTCTT78941.7029079299335577No Hit
GAATGATACCTGTCCCTTATACACATCTGACGCAATGACAGTCGTATGCC74651.6103632755198896No Hit
GAATCTTTCTCTTACCCACATCTGACGCAATGACAGTCGTATGCCGTCTT70511.5210544481836223No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCAATGACAGTCGTATG60771.3109414099577186No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA51191.1042799206143756No Hit
GAACTGTCCCTTATCCACATCTGACGCAATGACAGTCGTATGCCGTCTTC49911.0766675295538872No Hit
GAATCTGTCTCTTACCCACATCTGACGCAATGACAGTCGTATGCCGTCTT31370.6767193027871257No Hit
GAACTGTCCCTTATACACATCTGACGCAATGACAGTCGTATGCCGTCTTC28010.6042367762533437No Hit
GCTGTCTCCTATACCCATCTGACGCAATGACAGTCGTATGCCGTCTTCTG25540.5509534903788075No Hit
GAATGACTCTCTCTCCTACACATCTGACGCAATGACAGTCGTATGCCGTC25410.5481491069117267No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTATTTTTTTTTTTTTTTTTTTTTTTT24850.5360686858227629No Hit
GAATCTGTCTCTTATACACATCTGACGCAATGACAGTCGTATGCCGTCCT22900.4940029338165502No Hit
GAATGATACCTGGCCCTTATACACATCTGACGCAATGACAGTCGTATGCC22780.4914142721546294No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCAATGACAG22150.47782379842954525No Hit
GAATGACTCTCTCTTATACACATCTGACGCAATGACAGTCGTATGCCGTC19850.4282077832427302No Hit
GAATCTTTCTCTTATACACATCTGACGCAATGACAGTCGTATGCCGTCTT18450.39800673052032104No Hit
GAATGATCCGTCTCCCATACACATCTGACGCAATGACAGTCGTATGCCGT17820.3844162567952369No Hit
GAATGATACGGCGACCGTCTCTTATACACATCTGACGCAATGACAGTCGT16940.3654327379411511No Hit
GCTGTCTCCTATACACATCTGACGCAATGACAGTCGTATGCCGTCTTCTG16080.34688066269738543No Hit
GCTGTCTCTTATACACATCTGACGCAATGACAGTCGTATGCCGTCTTCTG14930.3220726551039779No Hit
GAATGCTGCCTCTTCCACACATCTGACGCAATGACAGTCGTATGCCGTCT12770.27547674518940374No Hit
GAATCTTTCTCTTACCCCCATCTGACGCAATGACAGTCGTATGCCGTCTT11590.250021572180516No Hit
GAACTGTCCCTTATACACATCTGACGCAATGACAGTCGTATGCCGTCCTC10600.2286651134696695No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCAATGACAGTCG10580.22823366985934937No Hit
CGTTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9500.2049357149020623No Hit
GAATGATACGGCGCCCTCTCTTATACACATCTGACGCAATGACAGTCGTA9280.20018983518854086No Hit
AAAAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8910.19220812839761844No Hit
GAATGATACGGCGACCACCCGTCTCTTATACACATCTGACGCAATGACAG8430.1818534817499353No Hit
GAATGCTGCCTCTTATACACATCTGACGCAATGACAGTCGTATGCCGTCT8370.18055915091897487No Hit
GAACTGTCTCTTATACACATCTGACGCAATGACAGTCGTATGCCGTCTTC7720.16653723358357062No Hit
GAATGATCCGTCTCTTATACACATCTGACGCAATGACAGTCGTATGCCGT7260.15661403054620762No Hit
GAATCTGTCTCTTACACACATCTGACGCAATGACAGTCGTATGCCGTCTT6690.1443178876520839No Hit
GAATGATACGGCTGCCTCTTATACAAATCTGACGCAATGACAGTCGTATG6270.1352575718353611No Hit
GAATGACTCGCTCTCCTACACATCTGACGCAATGACAGTCGTATGCCGTC6160.1328846319786004No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6050.13051169212183966No Hit
CGCTGTCTCTTATACCCATCTGACGCAATGACAGTCGTATGCCGTCTTCT5930.12792303045991887No Hit
CGTTTTTTCTTTTTCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5860.12641297782379843No Hit
GAATGATACGGCTGCCTCCTATACACATCTGACGCAATGACAGTCGTATG5670.12231426352575718No Hit
GAATGATACCTGGCTCTTATACACATCTGACGCAATGACAGTCGTATGCC5200.1121753386832341No Hit
GAATCTGTCTCTTATCCACATCTGACGCAATGACAGTCGTATGCCGTCTT5170.1115281732677539No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCAATGACAGTCGT5170.1115281732677539No Hit
GAATGATACGGCGACCGCCTCTTATACACATCTGACGCAATGACAGTCGT4720.10182069203555094No Hit
GAACTGTCCCTTATCCACCTCTGACGCAATGACAGTCGTATGCCGTCTTC4690.10117352662007074No Hit
AAAAAAAACAAAAACCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4690.10117352662007074No Hit
GAATCTTTCTCTTACACACATCTGACGCAATGACAGTCGTATGCCGTCTT4680.1009578048149107No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTGAAT254.438718E-544.020
CATGGTA207.851615E-444.044
CGGAATT700.044.09
GTGCAAG408.294592E-944.01
TTAGGCG351.4435864E-744.04
CGGAAAT207.851615E-444.021
ACCGGTG1500.044.017
ATTAGTC254.438718E-544.044
GTACATG207.851615E-444.041
TGCTTAG207.851615E-444.01
TTAGCTG454.783942E-1044.05
CGACGGG207.851615E-444.03
CACATAG207.851615E-444.034
CGTGAAA254.438718E-544.020
TGGGTAC351.4435864E-744.06
CGTTGAA302.5244353E-644.019
TAGCGGG700.044.03
TGAACGG351.4435864E-744.02
GTGATCC254.438718E-544.03
GTTAGCG207.851615E-444.01
Produced by FastQC (version 0.11.5)