FastQCFastQC Report
Thu 26 May 2016
SRR1546141_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546141_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences549251
Sequences flagged as poor quality0
Sequence length50
%GC34

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT485628.841495054173775No Hit
GAATCTGTCTCTTATACACATCTGACGCGAGCTCCATCGTATGCCGTCTT146712.6710920872242383No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA135622.4691807570673516No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGAGCTCCATCGTATGCC101631.8503380057569307No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGAGCTCCATCGTATG69581.266816082264757No Hit
GAACTGTCTCTTATACACATCTGACGCGAGCTCCATCGTATGCCGTCTTC37610.6847506877547788No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGAGCTCCATCGTATGCCGT19660.3579419973746065No Hit
GCTGTCTCTTATACACATCTGACGCGAGCTCCATCGTATGCCGTCTTCTG19650.35775993125183203No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA19570.35630340226963625No Hit
GAATGACTGTCTCTTATACACATCTGACGCGAGCTCCATCGTATGCCGTC18660.33973538509715956No Hit
GAATGCTGTCTCTTATACACATCTGACGCGAGCTCCATCGTATGCCGTCT16550.30131943319174653No Hit
GAATCTGTCTCTTATACACATCTGACGCGAGCTCCATCGTATGCCGTCCT14010.2550746380070314No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGAGCTCCA9120.16604430397031594No Hit
TGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9100.16568017172476698No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGAGCTCCATCGT8870.16149265090095422No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCGAGCTCCATCGTATGCCGTC8710.1585795929365627No Hit
CATTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8160.1485659561839669No Hit
GAATGATACCTGGCTCTTATACACATCTGACGCGAGCTCCATCGTATGCC7830.14255777413240941No Hit
TGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAAT7790.14182950964131152No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7000.12744628594212848No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCGAGCTCCATCG6380.11615818633011137No Hit
AGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAAT6210.1130630622429454No Hit
GGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAAT6080.1106962026468773No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGCGAA207.8528875E-444.031
CGACGAA302.525294E-644.019
GGCCGGA207.8528875E-444.035
GACGGTC351.4442412E-744.029
CGGCTAT351.4442412E-744.031
CGGTCTA351.4442412E-744.031
ATACCGG351.4442412E-744.028
CACCCGT302.525294E-644.016
CGTTTTT163700.043.489311
GAATCTG18900.042.0211641
AGGGTAC1750.041.4857146
CGGCTGT8650.040.9479759
ACGGCTG8800.040.7500048
CCGATGA3250.040.61538718
GTAATGG650.040.6153832
TACGGCT8850.040.519777
GATACCT12900.040.4186065
GTTTTTT178150.040.394052
AATCTGT19900.040.2412032
TGATACC13000.040.1076974
Produced by FastQC (version 0.11.5)