##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546141_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	549251
Sequences flagged as poor quality	0
Sequence length	50
%GC	34
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.010423285528837	31.0	28.0	31.0	25.0	33.0
2	28.86771985849821	31.0	28.0	31.0	23.0	33.0
3	28.266657684737943	31.0	27.0	31.0	16.0	34.0
4	31.658525883430343	33.0	30.0	35.0	25.0	37.0
5	33.58008633575542	35.0	33.0	35.0	30.0	37.0
6	33.697613659328795	35.0	33.0	36.0	29.0	37.0
7	33.371183666483994	35.0	33.0	36.0	28.0	37.0
8	33.48267367742617	35.0	33.0	36.0	28.0	37.0
9	35.22771556173771	37.0	34.0	39.0	29.0	39.0
10	35.65362466340525	37.0	35.0	39.0	30.0	39.0
11	35.801333088150955	37.0	35.0	39.0	30.0	39.0
12	35.422759357743544	37.0	34.0	39.0	29.0	39.0
13	35.573429998306786	38.0	35.0	39.0	30.0	39.0
14	37.01924438917726	39.0	36.0	41.0	31.0	41.0
15	36.86202665083905	39.0	35.0	41.0	30.0	41.0
16	36.73630635174082	39.0	35.0	41.0	30.0	41.0
17	36.3171245933098	38.0	34.0	40.0	29.0	41.0
18	35.52211830292526	38.0	34.0	39.0	27.0	40.0
19	34.626793578891984	37.0	33.0	38.0	27.0	40.0
20	34.44697051075009	35.0	34.0	38.0	27.0	40.0
21	34.948606374863225	35.0	34.0	39.0	30.0	40.0
22	35.19190679671043	35.0	34.0	39.0	30.0	40.0
23	35.2451502136546	35.0	34.0	39.0	30.0	40.0
24	35.0355866443575	35.0	34.0	39.0	30.0	40.0
25	34.80440272298093	35.0	34.0	39.0	27.0	40.0
26	34.5201392441707	35.0	33.0	39.0	27.0	40.0
27	34.822152349290214	35.0	34.0	39.0	29.0	40.0
28	34.715037387278315	35.0	34.0	39.0	27.0	40.0
29	34.53254522977655	35.0	33.0	39.0	26.0	40.0
30	34.178022434187646	35.0	33.0	39.0	25.0	40.0
31	33.54969403788068	35.0	33.0	38.0	22.0	40.0
32	33.12940349676195	35.0	33.0	39.0	20.0	40.0
33	32.387839075395405	35.0	32.0	39.0	15.0	40.0
34	31.64253501586706	35.0	31.0	39.0	12.0	40.0
35	30.950101137731203	35.0	29.0	39.0	8.0	40.0
36	30.638959237215772	35.0	27.0	39.0	8.0	40.0
37	30.454415194510343	35.0	25.0	39.0	8.0	40.0
38	30.232514824734046	35.0	24.0	39.0	7.0	40.0
39	29.92654724342787	35.0	23.0	38.0	7.0	40.0
40	29.581888790370886	35.0	23.0	38.0	7.0	40.0
41	29.001423757080097	35.0	22.0	38.0	7.0	40.0
42	29.29464124780838	35.0	22.0	38.0	7.0	40.0
43	29.220523949888122	35.0	22.0	38.0	7.0	40.0
44	29.08733711909491	35.0	21.0	38.0	7.0	40.0
45	29.16625185935028	35.0	22.0	38.0	7.0	40.0
46	28.712191693779346	34.0	20.0	38.0	7.0	40.0
47	28.602074461402893	34.0	20.0	38.0	7.0	40.0
48	28.663359738990007	34.0	21.0	38.0	7.0	40.0
49	28.482952238594013	34.0	20.0	37.0	7.0	40.0
50	28.526655390704796	34.0	20.0	38.0	7.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	5.0
13	8.0
14	26.0
15	110.0
16	258.0
17	692.0
18	1657.0
19	2793.0
20	3606.0
21	4740.0
22	6492.0
23	8738.0
24	12309.0
25	18011.0
26	25113.0
27	27851.0
28	25465.0
29	23408.0
30	23475.0
31	25983.0
32	30705.0
33	37316.0
34	49922.0
35	63090.0
36	45652.0
37	51595.0
38	50471.0
39	9759.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	29.522021807880183	17.743618127231446	14.406528162898201	38.327831901990166
2	47.873740785178356	28.948513521140605	14.71203511691376	8.46571057676727
3	17.841387635161336	27.363627922388854	45.99317980304087	8.80180463940894
4	16.250675920480802	18.21862864155004	55.25178834449095	10.27890709347821
5	19.825726307280277	20.582393113530973	45.56605267901197	14.025827900176784
6	12.204074275695447	32.87604392163146	47.104147284210676	7.815734518462415
7	41.60320145070286	10.440035612133615	43.895049804187884	4.061713132975634
8	38.49424033820603	16.30329302996262	39.37343764508394	5.8290289867474065
9	35.92747213933156	10.954372408971489	38.33384008404172	14.784315367655227
10	21.7224911743447	20.74861948362406	44.240065106845506	13.288824235185734
11	18.876069410888647	20.8125246927179	46.044704515786044	14.266701380607408
12	15.820817804610279	18.803060895656085	50.245880298806924	15.130241000926716
13	14.800883384827701	21.40915537704984	52.786977174370186	11.002984063752272
14	12.23029179737497	28.432720195320538	46.5042394096688	12.83274859763569
15	10.603166858139538	22.421807151921435	53.930534491516624	13.044491498422397
16	11.589965243577161	22.53195715619999	47.7992757409636	18.078801859259247
17	11.252232585830523	22.43873930133946	48.83213685546316	17.47689125736685
18	11.55282375453117	24.405053427303727	47.86281681781189	16.179306000353208
19	13.296471012342263	25.1611740351861	44.33310089558326	17.209254056888383
20	14.508485191651904	24.761538895696138	47.745930366990684	12.984045545661274
21	13.928422524492445	26.212059695840335	47.458630025252575	12.400887754414649
22	11.867069882439903	20.235010951277285	47.929088886501795	19.96883027978101
23	13.109307038130108	26.298176971912657	47.44479299992171	13.14772299003552
24	15.53570225634546	24.150707053787794	45.55840590185544	14.755184788011311
25	14.323141878667494	28.35060837394925	43.77215517131512	13.554094576068138
26	12.634660656057067	22.18038747312249	47.075744969057865	18.10920690176258
27	16.399423942787543	22.69326774097817	45.01439232700532	15.892915989228968
28	13.537344492772885	23.30755883921923	48.027222526677235	15.127874141330649
29	18.456953196261818	22.68926228627713	44.92081033989924	13.932974177561807
30	14.295831960251324	27.333404946008294	46.66172660586872	11.709036487871666
31	19.707201261354097	21.555718605883285	41.56187244083306	17.175207691929558
32	17.726503911690646	23.996497047797817	41.74157170401146	16.53542733650007
33	15.138616042574343	22.039468294095048	43.0926844011208	19.729231262209808
34	17.299922986030065	23.40532834714912	39.302431857201896	19.992316809618917
35	14.279992207569943	26.04437679676505	38.04653974230361	21.629091253361395
36	19.912025649475375	27.703727439731562	36.167435289148315	16.21681162164475
37	13.743989542121907	27.18483898982432	38.88586456829392	20.185306899759855
38	17.09965025097815	27.02899038872938	36.25519116032561	19.616168199966864
39	17.56191613670253	24.692535835164612	35.162248225310464	22.583299802822392
40	15.1103957935443	24.542877482244002	41.4375212789781	18.909205445233603
41	15.184314639390733	28.92739385089877	36.490784723195766	19.397506786514725
42	16.37411675172189	26.302182426613697	38.83069853309325	18.493002288571166
43	18.712756098759947	26.26777192940932	34.710724240829784	20.308747731000945
44	17.168653311509672	25.197223127495445	36.88022415981036	20.753899401184523
45	16.73624626992031	24.396860451778878	36.52137183182188	22.345521446478934
46	21.200143468104745	25.675146699778423	34.80503449242696	18.31967533968987
47	15.465060600708966	25.210331888335208	41.18372110383049	18.14088640712534
48	16.470611796792358	27.055025844286128	35.039899790806025	21.43446256811549
49	15.167382489972708	24.771188400203187	39.7131730301811	20.348256079643004
50	16.041846077658484	23.33213776579378	40.20893908249598	20.417077074051754
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	15895.0
1	12295.5
2	8696.0
3	36880.0
4	65064.0
5	41188.0
6	17312.0
7	15634.5
8	13957.0
9	13813.0
10	13669.0
11	13200.0
12	12731.0
13	12316.5
14	11902.0
15	11085.5
16	10269.0
17	9397.5
18	8526.0
19	7736.0
20	6946.0
21	6397.0
22	5848.0
23	5462.5
24	5077.0
25	4940.0
26	4803.0
27	4931.0
28	5059.0
29	5181.5
30	5304.0
31	5874.5
32	6445.0
33	6926.0
34	7407.0
35	8564.0
36	9721.0
37	10483.0
38	11245.0
39	12670.5
40	14096.0
41	15959.5
42	17823.0
43	19572.5
44	21322.0
45	22840.0
46	24358.0
47	43501.0
48	62644.0
49	53219.0
50	43794.0
51	37818.5
52	31843.0
53	25487.0
54	19131.0
55	16967.5
56	14804.0
57	13523.0
58	12242.0
59	11388.0
60	10534.0
61	9265.0
62	7996.0
63	7163.0
64	6330.0
65	5427.0
66	4524.0
67	4012.0
68	3500.0
69	3285.0
70	3070.0
71	2730.5
72	2391.0
73	1988.0
74	1585.0
75	1155.0
76	725.0
77	509.5
78	294.0
79	231.5
80	169.0
81	132.5
82	96.0
83	64.0
84	32.0
85	35.0
86	38.0
87	24.5
88	11.0
89	12.0
90	13.0
91	8.5
92	4.0
93	4.5
94	5.0
95	2.5
96	0.0
97	0.0
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	549251.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	38.23337903783596
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.80639607981496	29.7480143288756
2	7.7828558645552315	5.951297565327693
3	3.8187597074436135	4.380122620473218
4	2.4582341798618406	3.7594639664968628
5	1.7661095422492346	3.376216777557698
6	1.2850474015829525	2.9479022631784337
7	0.994522174043142	2.6616760269206883
8	0.7532773599054785	2.304027105750923
9	0.6246459833559097	2.1494093981497446
>10	2.573356757624073	15.144671675486174
>50	0.07769190515286832	2.091506559035706
>100	0.04623383312767309	3.7746253687577074
>500	0.0071495618238669725	1.9409807754858468
>1k	0.003336462184471254	2.655340426213902
>5k	4.766374549244648E-4	1.2679883800422984
>10k+	0.0019065498196978593	15.846756762247512
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	48562	8.841495054173775	No Hit
GAATCTGTCTCTTATACACATCTGACGCGAGCTCCATCGTATGCCGTCTT	14671	2.6710920872242383	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	13562	2.4691807570673516	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGAGCTCCATCGTATGCC	10163	1.8503380057569307	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGAGCTCCATCGTATG	6958	1.266816082264757	No Hit
GAACTGTCTCTTATACACATCTGACGCGAGCTCCATCGTATGCCGTCTTC	3761	0.6847506877547788	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGAGCTCCATCGTATGCCGT	1966	0.3579419973746065	No Hit
GCTGTCTCTTATACACATCTGACGCGAGCTCCATCGTATGCCGTCTTCTG	1965	0.35775993125183203	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1957	0.35630340226963625	No Hit
GAATGACTGTCTCTTATACACATCTGACGCGAGCTCCATCGTATGCCGTC	1866	0.33973538509715956	No Hit
GAATGCTGTCTCTTATACACATCTGACGCGAGCTCCATCGTATGCCGTCT	1655	0.30131943319174653	No Hit
GAATCTGTCTCTTATACACATCTGACGCGAGCTCCATCGTATGCCGTCCT	1401	0.2550746380070314	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGAGCTCCA	912	0.16604430397031594	No Hit
TGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	910	0.16568017172476698	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGAGCTCCATCGT	887	0.16149265090095422	No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCGAGCTCCATCGTATGCCGTC	871	0.1585795929365627	No Hit
CATTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	816	0.1485659561839669	No Hit
GAATGATACCTGGCTCTTATACACATCTGACGCGAGCTCCATCGTATGCC	783	0.14255777413240941	No Hit
TGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAAT	779	0.14182950964131152	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	700	0.12744628594212848	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCGAGCTCCATCG	638	0.11615818633011137	No Hit
AGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAAT	621	0.1130630622429454	No Hit
GGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAAT	608	0.1106962026468773	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.10377768998144746	0.0
2	0.0	0.0	0.0	0.7264438298701322	0.0
3	0.0	0.0	0.0	0.9252600359398526	0.0
4	0.0	0.0	0.0	1.9100556940269566	0.0
5	0.0	0.0	0.0	5.461619550988528	0.0
6	0.0	0.0	0.0	6.190066108209179	0.0
7	0.0	0.0	0.0	6.90503977234452	0.0
8	0.0	0.0	0.0	7.649508148369325	0.0
9	0.0	0.0	0.0	7.897664273710927	0.0
10	0.0	0.0	0.0	10.199344197825766	0.0
11	0.0	0.0	0.0	10.435119826818704	0.0
12	0.0	0.0	0.0	12.017456499851615	0.0
13	0.0	0.0	0.0	12.138894603742187	0.0
14	0.0	0.0	0.0	12.242126095355312	0.0
15	0.0	0.0	0.0	12.47098321168282	0.0
16	0.0	0.0	0.0	12.656144458544453	0.0
17	0.0	0.0	0.0	12.74189760237123	0.0
18	0.0	0.0	0.0	12.815270249849341	0.0
19	0.0	0.0	0.0	13.06415463968204	0.0
20	1.8206612277446924E-4	0.0	0.0	13.120231005496576	0.0
21	1.8206612277446924E-4	0.0	0.0	13.161195883120833	0.0
22	1.8206612277446924E-4	0.0	0.0	13.251682746139743	0.0
23	1.8206612277446924E-4	0.0	0.0	13.309397707059249	0.0
24	1.8206612277446924E-4	0.0	0.0	13.37293878390754	0.0
25	1.8206612277446924E-4	0.0	0.0	13.410262339076306	0.0
26	1.8206612277446924E-4	0.0	0.0	13.468887630609684	0.0
27	3.6413224554893847E-4	0.0	0.0	13.552273914840391	0.0
28	3.6413224554893847E-4	0.0	0.0	13.601613834112273	0.0
29	5.461983683234077E-4	0.0	0.0	13.654595075839643	0.0
30	5.461983683234077E-4	0.0	0.0	13.707576317567014	0.0
31	7.282644910978769E-4	0.0	0.0	13.751090120910112	0.0
32	9.103306138723462E-4	0.0	0.0	13.804799627128581	0.0
33	0.0010923967366468154	0.0	0.0	13.862696654170861	0.0
34	0.0010923967366468154	0.0	0.0	13.92004748284482	0.0
35	0.0010923967366468154	0.0	0.0	13.989596741744666	0.0
36	0.0012744628594212846	0.0	0.0	14.03820839652545	0.0
37	0.0012744628594212846	0.0	0.0	14.089915175393399	0.0
38	0.0012744628594212846	0.0	0.0	14.155641045714983	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGCGAA	20	7.8528875E-4	44.0	31
CGACGAA	30	2.525294E-6	44.0	19
GGCCGGA	20	7.8528875E-4	44.0	35
GACGGTC	35	1.4442412E-7	44.0	29
CGGCTAT	35	1.4442412E-7	44.0	31
CGGTCTA	35	1.4442412E-7	44.0	31
ATACCGG	35	1.4442412E-7	44.0	28
CACCCGT	30	2.525294E-6	44.0	16
CGTTTTT	16370	0.0	43.48931	1
GAATCTG	1890	0.0	42.021164	1
AGGGTAC	175	0.0	41.485714	6
CGGCTGT	865	0.0	40.947975	9
ACGGCTG	880	0.0	40.750004	8
CCGATGA	325	0.0	40.615387	18
GTAATGG	65	0.0	40.615383	2
TACGGCT	885	0.0	40.51977	7
GATACCT	1290	0.0	40.418606	5
GTTTTTT	17815	0.0	40.39405	2
AATCTGT	1990	0.0	40.241203	2
TGATACC	1300	0.0	40.107697	4
>>END_MODULE