FastQCFastQC Report
Thu 26 May 2016
SRR1546140_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546140_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences554390
Sequences flagged as poor quality0
Sequence length50
%GC34

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT496878.962463247894082No Hit
GAATCTGTCTCTTATACACATCTGACGCGAGCTCCATCGTATGCCGTCTT148342.6757336892801096No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA130392.351954400331896No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGAGCTCCATCGTATGCC98511.7769079528851532No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGAGCTCCATCGTATG72511.3079240246036183No Hit
GAACTGTCTCTTATACACATCTGACGCGAGCTCCATCGTATGCCGTCTTC39340.7096087591767528No Hit
GCTGTCTCTTATACACATCTGACGCGAGCTCCATCGTATGCCGTCTTCTG19860.35823156983351073No Hit
GAATGACTGTCTCTTATACACATCTGACGCGAGCTCCATCGTATGCCGTC19310.34831075596601674No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGAGCTCCATCGTATGCCGT19270.347589242230199No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA18980.3423582676455203No Hit
GAATGCTGTCTCTTATACACATCTGACGCGAGCTCCATCGTATGCCGTCT16680.30087122783599995No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGAGCTCCA10200.18398600263352513No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGAGCTCCATCGT9690.17478670250184888No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCGAGCTCCATCGTATGCCGTC8790.1585526434459496No Hit
TGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8270.14917296488031892No Hit
TGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAAT8230.14845145114450117No Hit
GAATGATACCTGGCTCTTATACACATCTGACGCGAGCTCCATCGTATGCC7950.14340085499377694No Hit
CATTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7800.1406951784844604No Hit
GAATCTGTCTCTTATACACATCTGACGCGAGCTCCATCGTATGCCGTCCT7410.1336604195602374No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCGAGCTCCATCG6830.12319847039088005No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6670.12031241544760908No Hit
AGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAAT6240.11255614278756831No Hit
GGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAAT5720.10317646422193763No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGGCTA408.29823E-944.030
CCATCGA207.852951E-444.041
AGACCCG302.5253357E-644.039
GTCGTAC207.852951E-444.09
TCGTAGG254.4398486E-544.02
CGGCTAT408.29823E-944.031
CCAGTCG254.4398486E-544.028
CGTTTTT160050.043.4639171
GATACCT12000.041.85
CGATGAA4650.041.6344119
CCGATGA4600.041.60869618
TGATACC12350.041.14984
GAATCTG20750.040.92531
CGACGGG650.040.6153873
CGGCTGT8700.040.459779
ACGGTCT603.6379788E-1240.33333230
GTTTTTT174800.040.2494282
AAGGGCT1700.040.117655
AGGGCGC1700.040.117656
CGGTCTA557.8216544E-1140.031
Produced by FastQC (version 0.11.5)