##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546140_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	554390
Sequences flagged as poor quality	0
Sequence length	50
%GC	34
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.00020563141471	30.0	28.0	31.0	25.0	33.0
2	28.84864986742185	31.0	28.0	31.0	22.0	33.0
3	28.27620267320839	31.0	28.0	31.0	16.0	34.0
4	31.628752322372335	33.0	30.0	35.0	25.0	37.0
5	33.59321235953029	35.0	33.0	35.0	30.0	37.0
6	33.77653998087989	35.0	33.0	36.0	30.0	37.0
7	33.43190894496654	35.0	33.0	36.0	28.0	37.0
8	33.54121647215859	35.0	33.0	37.0	29.0	37.0
9	35.35259654755677	37.0	34.0	39.0	29.0	39.0
10	35.67294323490683	37.0	35.0	39.0	30.0	39.0
11	35.86485506592832	37.0	35.0	39.0	30.0	39.0
12	35.3711809376071	37.0	34.0	39.0	29.0	39.0
13	35.57526290156749	38.0	35.0	39.0	30.0	39.0
14	37.00634390952218	39.0	36.0	41.0	31.0	41.0
15	36.916715669474556	39.0	35.0	41.0	30.0	41.0
16	36.78094482223705	39.0	35.0	41.0	31.0	41.0
17	36.364469056079656	38.0	34.0	40.0	30.0	41.0
18	35.59393748083479	38.0	34.0	39.0	28.0	40.0
19	34.67324446689154	37.0	33.0	38.0	27.0	40.0
20	34.40558451631523	35.0	33.0	38.0	27.0	40.0
21	34.807882537563806	35.0	33.0	39.0	29.0	40.0
22	35.134641678240946	35.0	34.0	39.0	30.0	40.0
23	35.23476794314472	35.0	34.0	39.0	30.0	40.0
24	35.00113458034957	35.0	34.0	39.0	30.0	40.0
25	34.753015025523545	35.0	34.0	39.0	27.0	40.0
26	34.453518281354285	35.0	33.0	39.0	27.0	40.0
27	34.805158823211094	35.0	34.0	39.0	29.0	40.0
28	34.70290770035535	35.0	34.0	39.0	27.0	40.0
29	34.520999657280974	35.0	33.0	39.0	26.0	40.0
30	34.20554483305976	35.0	33.0	39.0	25.0	40.0
31	33.5909594328902	35.0	33.0	38.0	23.0	40.0
32	33.20499648262054	35.0	33.0	39.0	21.0	40.0
33	32.44371471346886	35.0	32.0	39.0	16.0	40.0
34	31.627249770017496	35.0	31.0	39.0	12.0	40.0
35	30.974876891718825	35.0	29.0	39.0	8.0	40.0
36	30.664977723263405	35.0	27.0	39.0	8.0	40.0
37	30.451039881671747	35.0	25.0	39.0	8.0	40.0
38	30.25276971085337	35.0	24.0	39.0	7.0	40.0
39	29.937913743032883	35.0	23.0	38.0	7.0	40.0
40	29.532076696910117	35.0	23.0	38.0	7.0	40.0
41	28.95079997835459	35.0	21.0	38.0	7.0	40.0
42	29.265035444362272	35.0	22.0	38.0	7.0	40.0
43	29.20842186908133	35.0	22.0	38.0	7.0	40.0
44	29.088933783076897	35.0	22.0	38.0	7.0	40.0
45	29.16298634535255	35.0	22.0	38.0	7.0	40.0
46	28.706242897599164	34.0	21.0	38.0	7.0	40.0
47	28.58016378361803	34.0	20.0	37.0	7.0	40.0
48	28.62474972492289	34.0	20.0	37.0	7.0	40.0
49	28.578735186421113	34.0	20.0	37.0	7.0	40.0
50	28.590753801475497	34.0	20.0	38.0	7.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	3.0
11	0.0
12	8.0
13	9.0
14	34.0
15	83.0
16	268.0
17	744.0
18	1568.0
19	2682.0
20	3505.0
21	4797.0
22	6398.0
23	8822.0
24	12393.0
25	18336.0
26	25285.0
27	28384.0
28	25854.0
29	23343.0
30	23725.0
31	26360.0
32	30454.0
33	38177.0
34	50479.0
35	63405.0
36	46416.0
37	52969.0
38	50830.0
39	9058.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	29.46048810404228	17.579321416331464	14.358664478075001	38.60152600155126
2	48.086725951045295	28.814192175183535	14.657371164703548	8.441710709067623
3	17.95396742365483	27.207561463951368	46.001731632965964	8.83673947942784
4	16.203033965259113	18.088349356950882	55.310521474052564	10.39809520373744
5	19.93740868341781	20.539331517523767	45.635202655170545	13.888057143887877
6	12.20205992171576	32.79099550857699	47.27881094536337	7.728133624343873
7	41.39179999639243	10.29401684734573	44.24755136275907	4.0666317935027685
8	38.39517307310738	16.298634535254966	39.50486119879507	5.801331192842583
9	35.81738487346453	10.835332527642995	38.66159202005808	14.685690578834395
10	21.881888201446635	20.35660816392792	44.326015981529245	13.435487653096196
11	19.16683201356446	20.401522393982578	46.29935604899078	14.132289543462184
12	15.809628600804487	18.29307887948917	50.14664766680495	15.750644852901388
13	15.03436209166832	21.13061202402641	52.909684518119015	10.92534136618626
14	12.340590558992767	28.454337199444435	46.642796587240035	12.562275654322768
15	10.507224156279875	22.154800772019698	53.97914825303487	13.358826818665559
16	11.586067569761358	22.14506033658616	48.048124966179046	18.220747127473437
17	11.227114486192031	22.46108335287433	48.98933963455329	17.32246252638035
18	11.50958711376468	24.07240390338931	47.90201843467595	16.515990548170063
19	13.29948231389455	24.900521293674128	44.61786828766753	17.182128104763795
20	14.73836108154909	24.223380652609176	47.86882880282833	13.169429463013401
21	13.854145998304443	26.202673208391204	47.555331084615524	12.38784970868883
22	11.93095113548224	19.92027273219214	48.48464077634878	19.66413535597684
23	13.334295351647757	25.923988527931602	47.594653583217585	13.147062537203052
24	15.415501722614044	24.01017334367503	45.76543588448565	14.808889049225273
25	14.505492523313912	28.030628258085464	44.06789444254045	13.395984776060175
26	12.689081693392737	22.014466350403143	47.39569617056585	17.90075578563827
27	16.52086076588683	22.391276898933963	45.23187647684843	15.85598585833078
28	13.563375962769891	23.13281264092065	48.304803477696204	14.99900791861325
29	18.20126625660636	22.348166453218855	45.5071339670629	13.94343332311189
30	14.162953877234438	27.1801439419903	46.80441566406321	11.852486516712062
31	19.65150886560003	21.33047132884792	41.87503382095636	17.142985984595683
32	17.775392774039936	23.92810115622576	42.008513862082644	16.28799220765165
33	15.277692599072854	21.733797507170046	43.279099550857694	19.709410342899403
34	17.135410090369597	23.250599758292896	39.60064214722488	20.013348004112625
35	14.243402658778118	25.835603095293923	38.39733761431483	21.523656631613125
36	19.848121358610367	27.505366258410145	36.47197821028518	16.17453417269431
37	13.7284222298382	26.90542758707769	39.18180342358268	20.184346759501434
38	17.105467270333158	26.892620718266926	36.62818593408972	19.373726077310195
39	17.502480203466874	24.66061797651473	35.303306336694384	22.533595483324014
40	15.140785367701437	24.428831688883275	41.52996987680153	18.900413066613755
41	15.194718519453815	28.639766229549597	36.64117318133444	19.52434206966215
42	16.37980483053446	26.27698912318043	38.80174606324068	18.54145998304443
43	18.62966503724815	26.33633362795144	34.861379173506016	20.172622161294395
44	17.261133858835837	25.023178628763144	37.06398023052364	20.65170728187738
45	16.765453922329048	24.22572557225058	36.814336477930695	22.194484027489672
46	21.191399556269054	25.56323166002273	35.12094373996645	18.12442504374177
47	15.471419037139919	25.26741102833745	41.34291744079078	17.91825249373185
48	16.53186385035805	26.94204440917044	35.2268258806977	21.299265859773804
49	15.30673352693952	24.68587095726835	40.11273652122152	19.89465899457061
50	16.13827810746947	23.2201158029546	40.27110878623352	20.370497303342415
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	15389.0
1	11938.0
2	8487.0
3	37489.0
4	66491.0
5	42018.5
6	17546.0
7	15852.5
8	14159.0
9	13958.0
10	13757.0
11	13359.0
12	12961.0
13	12410.5
14	11860.0
15	11033.0
16	10206.0
17	9374.5
18	8543.0
19	7842.5
20	7142.0
21	6605.0
22	6068.0
23	5648.5
24	5229.0
25	4986.5
26	4744.0
27	4897.0
28	5050.0
29	5183.5
30	5317.0
31	5886.5
32	6456.0
33	7011.0
34	7566.0
35	8596.0
36	9626.0
37	10676.5
38	11727.0
39	13073.5
40	14420.0
41	16347.5
42	18275.0
43	20010.5
44	21746.0
45	23079.5
46	24413.0
47	43654.0
48	62895.0
49	53299.0
50	43703.0
51	37815.0
52	31927.0
53	25765.0
54	19603.0
55	17415.0
56	15227.0
57	13990.5
58	12754.0
59	11657.5
60	10561.0
61	9309.0
62	8057.0
63	7167.5
64	6278.0
65	5385.5
66	4493.0
67	3989.5
68	3486.0
69	3217.5
70	2949.0
71	2644.5
72	2340.0
73	1943.0
74	1546.0
75	1141.5
76	737.0
77	515.0
78	293.0
79	221.0
80	149.0
81	127.5
82	106.0
83	80.0
84	54.0
85	42.5
86	31.0
87	20.0
88	9.0
89	7.0
90	5.0
91	6.5
92	8.0
93	4.0
94	0.0
95	0.0
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	554390.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	37.94350933048346
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.39015105113023	29.36453918496084
2	7.942432522150268	6.02727525021888
3	3.899966639729598	4.4393526174946265
4	2.4668342339772575	3.744013910946884
5	1.8178154686310641	3.448714909754997
6	1.309905643134613	2.982145019539867
7	0.9973843184365238	2.649098283487189
8	0.7843404199301457	2.380850243351584
9	0.6116698582908097	2.0888010877709546
>10	2.6415471791803395	15.384966761337063
>50	0.07780721267027299	2.040513609412963
>100	0.0467797965746593	3.712264063417059
>500	0.00763751780810764	1.990524105042617
>1k	0.0033414140410470924	2.601627683788185
>5k	9.54689726013455E-4	3.0975380568188204
>10k+	0.0014320345890201827	14.04777521265745
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	49687	8.962463247894082	No Hit
GAATCTGTCTCTTATACACATCTGACGCGAGCTCCATCGTATGCCGTCTT	14834	2.6757336892801096	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	13039	2.351954400331896	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGAGCTCCATCGTATGCC	9851	1.7769079528851532	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGAGCTCCATCGTATG	7251	1.3079240246036183	No Hit
GAACTGTCTCTTATACACATCTGACGCGAGCTCCATCGTATGCCGTCTTC	3934	0.7096087591767528	No Hit
GCTGTCTCTTATACACATCTGACGCGAGCTCCATCGTATGCCGTCTTCTG	1986	0.35823156983351073	No Hit
GAATGACTGTCTCTTATACACATCTGACGCGAGCTCCATCGTATGCCGTC	1931	0.34831075596601674	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGAGCTCCATCGTATGCCGT	1927	0.347589242230199	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1898	0.3423582676455203	No Hit
GAATGCTGTCTCTTATACACATCTGACGCGAGCTCCATCGTATGCCGTCT	1668	0.30087122783599995	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGAGCTCCA	1020	0.18398600263352513	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGAGCTCCATCGT	969	0.17478670250184888	No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCGAGCTCCATCGTATGCCGTC	879	0.1585526434459496	No Hit
TGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	827	0.14917296488031892	No Hit
TGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAAT	823	0.14845145114450117	No Hit
GAATGATACCTGGCTCTTATACACATCTGACGCGAGCTCCATCGTATGCC	795	0.14340085499377694	No Hit
CATTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	780	0.1406951784844604	No Hit
GAATCTGTCTCTTATACACATCTGACGCGAGCTCCATCGTATGCCGTCCT	741	0.1336604195602374	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCGAGCTCCATCG	683	0.12319847039088005	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	667	0.12031241544760908	No Hit
AGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAAT	624	0.11255614278756831	No Hit
GGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAAT	572	0.10317646422193763	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.11472068399502156	0.0
2	0.0	0.0	0.0	0.6949981060264435	0.0
3	0.0	0.0	0.0	0.896480816753549	0.0
4	0.0	0.0	0.0	1.8786413896354552	0.0
5	0.0	0.0	0.0	5.316293583939104	0.0
6	0.0	0.0	0.0	6.073341871245874	0.0
7	0.0	0.0	0.0	6.768700734140226	0.0
8	0.0	0.0	0.0	7.482458197297931	0.0
9	0.0	0.0	0.0	7.728855138079691	0.0
10	0.0	0.0	0.0	9.933440357870813	0.0
11	1.803784339544364E-4	0.0	0.0	10.163963996464583	0.0
12	1.803784339544364E-4	0.0	0.0	11.779794007828425	0.0
13	3.607568679088728E-4	0.0	0.0	11.899745666408124	0.0
14	3.607568679088728E-4	0.0	0.0	11.998593048215156	0.0
15	3.607568679088728E-4	0.0	0.0	12.23993939284619	0.0
16	3.607568679088728E-4	0.0	0.0	12.426270315121124	0.0
17	3.607568679088728E-4	0.0	0.0	12.510867800645755	0.0
18	3.607568679088728E-4	0.0	0.0	12.597449448943884	0.0
19	3.607568679088728E-4	0.0	0.0	12.871263911686718	0.0
20	3.607568679088728E-4	0.0	0.0	12.927902739948411	0.0
21	3.607568679088728E-4	0.0	0.0	12.973538483738885	0.0
22	3.607568679088728E-4	0.0	0.0	13.072927000847779	0.0
23	3.607568679088728E-4	0.0	0.0	13.129385450675517	0.0
24	3.607568679088728E-4	0.0	0.0	13.193600173163297	0.0
25	3.607568679088728E-4	0.0	0.0	13.230036616822092	0.0
26	3.607568679088728E-4	0.0	0.0	13.2913652843666	0.0
27	3.607568679088728E-4	0.0	0.0	13.394361370154584	0.0
28	5.411353018633092E-4	0.0	0.0	13.444326196359963	0.0
29	5.411353018633092E-4	0.0	0.0	13.494651779433251	0.0
30	5.411353018633092E-4	0.0	0.0	13.544796984072585	0.0
31	5.411353018633092E-4	0.0	0.0	13.592056133768647	0.0
32	5.411353018633092E-4	0.0	0.0	13.647973448294522	0.0
33	5.411353018633092E-4	0.0	0.0	13.700643951009218	0.0
34	5.411353018633092E-4	0.0	0.0	13.76251375385559	0.0
35	5.411353018633092E-4	0.0	0.0	13.829794909720594	0.0
36	5.411353018633092E-4	0.0	0.0	13.874709139775248	0.0
37	5.411353018633092E-4	0.0	0.0	13.921607532603401	0.0
38	5.411353018633092E-4	0.0	0.0	13.983116578581864	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGGCTA	40	8.29823E-9	44.0	30
CCATCGA	20	7.852951E-4	44.0	41
AGACCCG	30	2.5253357E-6	44.0	39
GTCGTAC	20	7.852951E-4	44.0	9
TCGTAGG	25	4.4398486E-5	44.0	2
CGGCTAT	40	8.29823E-9	44.0	31
CCAGTCG	25	4.4398486E-5	44.0	28
CGTTTTT	16005	0.0	43.463917	1
GATACCT	1200	0.0	41.8	5
CGATGAA	465	0.0	41.63441	19
CCGATGA	460	0.0	41.608696	18
TGATACC	1235	0.0	41.1498	4
GAATCTG	2075	0.0	40.9253	1
CGACGGG	65	0.0	40.615387	3
CGGCTGT	870	0.0	40.45977	9
ACGGTCT	60	3.6379788E-12	40.333332	30
GTTTTTT	17480	0.0	40.249428	2
AAGGGCT	170	0.0	40.11765	5
AGGGCGC	170	0.0	40.11765	6
CGGTCTA	55	7.8216544E-11	40.0	31
>>END_MODULE