FastQCFastQC Report
Thu 26 May 2016
SRR1546139_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546139_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences548948
Sequences flagged as poor quality0
Sequence length50
%GC34

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT494069.000123873299474No Hit
GAATCTGTCTCTTATACACATCTGACGCGAGCTCCATCGTATGCCGTCTT147462.6862289324307587No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA129852.3654335201148378No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGAGCTCCATCGTATGCC99121.8056355064596283No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGAGCTCCATCGTATG69681.2693369863812236No Hit
GAACTGTCTCTTATACACATCTGACGCGAGCTCCATCGTATGCCGTCTTC37870.6898649781035726No Hit
GCTGTCTCTTATACACATCTGACGCGAGCTCCATCGTATGCCGTCTTCTG20610.37544539737825805No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20190.36779439946953085No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGAGCTCCATCGTATGCCGT19120.3483025714639638No Hit
GAATGACTGTCTCTTATACACATCTGACGCGAGCTCCATCGTATGCCGTC19030.34666307191209367No Hit
GAATGCTGTCTCTTATACACATCTGACGCGAGCTCCATCGTATGCCGTCT17260.3144195807253146No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGAGCTCCA11590.21113110895749687No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGAGCTCCATCGT9850.17943411762134118No Hit
TGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8240.15010529230455344No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCGAGCTCCATCGTATGCCGTC8240.15010529230455344No Hit
GAATGATACCTGGCTCTTATACACATCTGACGCGAGCTCCATCGTATGCC8230.149923125687679No Hit
TGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAAT8090.14737279305143655No Hit
CATTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7960.14500462703206862No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCGAGCTCCATCG6960.1267879653446228No Hit
GGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAAT6400.11658663479965316No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6360.11585796833215532No Hit
AGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAAT6250.11385413554653627No Hit
CGCTGTCTCTTATACACATCTGACGCGAGCTCCATCGTATGCCGTCTTCT5570.10146680559907315No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACGAGG207.852883E-444.0000042
TATAGCG207.852883E-444.0000041
CCGCAAT207.852883E-444.00000433
TTCGTAG302.5252903E-644.01
GACGGTC302.5252903E-644.029
CGGTCTA302.5252903E-644.031
CGGACGG302.5252903E-644.02
ATAGCGG454.783942E-1043.9999962
TAGCCGT454.783942E-1043.99999644
CGTTTTT156200.043.5070421
ATGATCT2250.042.0444453
CCGATGA6250.041.53618
AATGATC2350.041.1914862
GAATCTG16750.040.9791031
CGGCTGT7950.040.9559759
CTTCGGA700.040.85714313
ATGCGGG700.040.8571433
ACCGACT650.040.61538717
AATCTGT17300.040.5664752
ACGGCTG8050.040.447218
Produced by FastQC (version 0.11.5)