##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546139_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	548948
Sequences flagged as poor quality	0
Sequence length	50
%GC	34
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.89785917791849	30.0	28.0	31.0	25.0	33.0
2	28.74866471869831	31.0	28.0	31.0	16.0	33.0
3	28.17904428106123	31.0	27.0	31.0	16.0	33.0
4	31.572591575158302	33.0	30.0	35.0	25.0	37.0
5	33.56271450119137	35.0	33.0	35.0	30.0	37.0
6	33.72802341934027	35.0	33.0	36.0	30.0	37.0
7	33.31608822693588	35.0	32.0	36.0	28.0	37.0
8	33.47852073420433	35.0	33.0	36.0	28.0	37.0
9	35.29136275202752	37.0	34.0	39.0	29.0	39.0
10	35.70141069828107	37.0	35.0	39.0	30.0	39.0
11	35.87603926054927	37.0	35.0	39.0	30.0	39.0
12	35.52962029190379	37.0	34.0	39.0	30.0	39.0
13	35.62967894955442	38.0	35.0	39.0	30.0	39.0
14	37.0541162368749	39.0	36.0	41.0	31.0	41.0
15	36.87285680975247	39.0	35.0	41.0	30.0	41.0
16	36.73434095761347	39.0	35.0	41.0	30.0	41.0
17	36.31323003271712	38.0	34.0	40.0	29.0	41.0
18	35.52940533529588	38.0	34.0	39.0	27.0	40.0
19	34.57646443743305	37.0	33.0	38.0	27.0	40.0
20	34.44658692626624	35.0	34.0	38.0	27.0	40.0
21	34.95091520508318	35.0	34.0	39.0	30.0	40.0
22	35.14399906730692	35.0	34.0	39.0	30.0	40.0
23	35.207921333168166	35.0	34.0	39.0	30.0	40.0
24	35.012469304925055	35.0	34.0	39.0	30.0	40.0
25	34.773386550274346	35.0	34.0	39.0	27.0	40.0
26	34.45900704620474	35.0	33.0	39.0	27.0	40.0
27	34.814051604159225	35.0	34.0	39.0	28.0	40.0
28	34.709810400985155	35.0	34.0	39.0	27.0	40.0
29	34.50686403812383	35.0	33.0	39.0	26.0	41.0
30	34.20393006259245	35.0	33.0	39.0	25.0	40.0
31	33.564609034006864	35.0	33.0	38.0	23.0	40.0
32	33.194176861924994	35.0	33.0	39.0	21.0	40.0
33	32.427477648156106	35.0	32.0	39.0	15.0	40.0
34	31.616981936358272	35.0	31.0	39.0	12.0	40.0
35	30.927195289899956	35.0	29.0	39.0	8.0	40.0
36	30.678004109678877	35.0	27.0	39.0	8.0	40.0
37	30.480557356980988	35.0	25.0	39.0	8.0	40.0
38	30.271304385843468	35.0	24.0	39.0	7.0	40.0
39	29.935930179179085	35.0	23.0	38.0	7.0	40.0
40	29.582778332373923	35.0	23.0	38.0	7.0	40.0
41	29.00661446985871	35.0	22.0	38.0	7.0	40.0
42	29.353732958312992	35.0	23.0	38.0	7.0	40.0
43	29.300053921318593	35.0	22.0	38.0	7.0	40.0
44	29.179858565838657	35.0	22.0	38.0	7.0	40.0
45	29.26835875164861	35.0	23.0	38.0	7.0	40.0
46	28.82465187959515	34.0	22.0	38.0	7.0	40.0
47	28.739862427770937	34.0	21.0	38.0	7.0	40.0
48	28.755568833477852	34.0	22.0	38.0	7.0	40.0
49	28.739505381201862	34.0	21.0	38.0	7.0	40.0
50	28.66958436864694	34.0	20.0	38.0	7.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	3.0
11	1.0
12	1.0
13	20.0
14	36.0
15	86.0
16	241.0
17	643.0
18	1497.0
19	2640.0
20	3534.0
21	4790.0
22	6610.0
23	8674.0
24	12329.0
25	18199.0
26	25193.0
27	27575.0
28	25359.0
29	23094.0
30	23278.0
31	26155.0
32	30457.0
33	37490.0
34	49971.0
35	63045.0
36	45511.0
37	51701.0
38	50645.0
39	10170.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	29.549975589673338	17.615511851760093	14.373128238011615	38.461384320554956
2	47.94661789459111	28.81256512456553	14.743837303351137	8.49697967749222
3	17.976748253022144	27.203851730947193	45.97903626573009	8.840363750300575
4	16.258188389428508	18.137965708956038	55.13782726232721	10.466018639288238
5	20.13487616313385	20.675364515400364	45.36495260024629	13.824806721219495
6	12.298068305194663	32.820959362271104	46.945065835015335	7.935906497518891
7	41.29535037927089	10.382586328759738	44.19034225463979	4.131721037329584
8	38.389975006740165	16.473873663807865	39.293339259820605	5.842812069631368
9	35.754024060566756	10.938923176694331	38.538805132726594	14.768247630012315
10	21.897884681244854	20.702325174697787	44.080495784664485	13.319294359392874
11	19.19016008802291	20.52598788956331	46.10819239709408	14.175659625319703
12	15.82262800848168	18.678818394456307	49.94571434817141	15.552839248890605
13	15.14150702798808	21.295277512624146	52.66600843795769	10.89720702143008
14	12.306994469421511	28.491951878866484	46.505497788497266	12.69555586321473
15	10.572221776926048	22.34200689318478	53.63276667371044	13.453004656178727
16	11.63589265285601	22.189351268243986	47.85462375306951	18.3201323258305
17	11.308357075715733	22.710712125738684	48.685667859250785	17.295262939294798
18	11.575959835904312	24.110662576418896	47.73494028578299	16.578437301893803
19	13.267923373434279	25.055742984763583	44.34973804440494	17.326595597397205
20	14.884105598344469	24.31596435363641	47.695227963304355	13.104702084714765
21	14.02846899888514	26.20412133754017	47.38445171491653	12.382957948658161
22	11.874348754344673	20.08223001085713	48.38855410712854	19.65486712766965
23	13.344251185904676	26.021043887581335	47.320329065776725	13.314375860737265
24	15.361382134555551	24.205389217193616	45.644760523765456	14.78846812448538
25	14.503013035843104	28.13144414407193	43.81890452283276	13.546638297252198
26	12.66732003759919	22.057644804243754	47.29245757339493	17.982577584762126
27	16.490815159177192	22.50704984807304	45.184243316306826	15.81789167644294
28	13.646283436682527	23.24318514686273	48.040433702281454	15.070097714173292
29	18.247812178931337	22.401939710136478	45.34928627119509	14.000961839737098
30	14.243607773413874	27.216785560745283	46.69986956870232	11.839737097138526
31	19.502575835962606	21.454126802538674	41.68227227351225	17.361025087986476
32	17.91280777049921	23.876942806968966	41.99195552219883	16.218293900333
33	15.279953656812667	21.951805999839692	43.05216523240817	19.716075110939467
34	17.117468321225328	23.469982584871428	39.465304546150094	19.947244547753158
35	14.344892412396074	25.98515706405707	38.10652375088351	21.563426772663348
36	19.943783382032542	27.50861648097816	36.35517389625247	16.192426240736825
37	13.848306214796303	27.03297944431895	38.94612968805789	20.17258465282686
38	17.22221412592814	26.853727493314484	36.47868286249335	19.445375518264026
39	17.480344222039246	24.738590904785152	35.23320970292268	22.54785517025292
40	15.081209877802634	24.55697078776132	41.37823618994877	18.983583144487273
41	15.364843300276165	28.55188469581818	36.675058475484015	19.408213528421637
42	16.4115726808368	26.417620612517034	38.665775264688094	18.50503144195807
43	18.411944300735225	26.451139270021933	34.83900114400635	20.29791528523649
44	17.34317275953278	25.134621129870226	37.07199953365346	20.450206576943536
45	16.849501227803	24.299387191500834	36.781625946355575	22.069485634340595
46	21.296917012176017	25.647420156371826	35.0433920881395	18.012270743312662
47	15.518774091535082	25.37744923016388	41.28259871608969	17.821177962211358
48	16.611773792781833	27.031522111383953	35.11225106931804	21.244453026516172
49	15.267566326865204	24.769012729803187	40.21346284165349	19.749958101678118
50	16.14524508696634	23.32880345679372	40.17411485240861	20.351836603831327
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	15236.0
1	11846.0
2	8456.0
3	37210.0
4	65964.0
5	41516.5
6	17069.0
7	15507.5
8	13946.0
9	13768.5
10	13591.0
11	13112.0
12	12633.0
13	12149.0
14	11665.0
15	10733.0
16	9801.0
17	9085.5
18	8370.0
19	7773.0
20	7176.0
21	6577.0
22	5978.0
23	5525.5
24	5073.0
25	4825.5
26	4578.0
27	4751.0
28	4924.0
29	5064.5
30	5205.0
31	5837.5
32	6470.0
33	6994.0
34	7518.0
35	8742.5
36	9967.0
37	10639.0
38	11311.0
39	12948.5
40	14586.0
41	16425.5
42	18265.0
43	19994.0
44	21723.0
45	23148.5
46	24574.0
47	43611.0
48	62648.0
49	52929.0
50	43210.0
51	37446.5
52	31683.0
53	25535.5
54	19388.0
55	17149.0
56	14910.0
57	13777.5
58	12645.0
59	11562.5
60	10480.0
61	9098.5
62	7717.0
63	6989.0
64	6261.0
65	5394.5
66	4528.0
67	3931.0
68	3334.0
69	3078.5
70	2823.0
71	2583.5
72	2344.0
73	1912.5
74	1481.0
75	1091.0
76	701.0
77	510.0
78	319.0
79	249.0
80	179.0
81	156.0
82	133.0
83	87.5
84	42.0
85	27.0
86	12.0
87	12.5
88	13.0
89	12.0
90	11.0
91	7.0
92	3.0
93	2.0
94	1.0
95	1.5
96	2.0
97	1.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	548948.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	37.87984869422486
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.3720950551843	29.308432538455655
2	7.883333045364044	5.972389259291458
3	3.783816385990204	4.29991176564113
4	2.5464164265737406	3.8583147580440817
5	1.808190647071681	3.424699406069391
6	1.3785566255880777	3.1331709836218544
7	1.0265997757230496	2.722121092174001
8	0.7748339304454	2.3480473638738646
9	0.6211880318500711	2.1177457791429632
>10	2.6730958745254907	15.498853258026172
>50	0.07171261332452647	1.9201199803521425
>100	0.04716668527379318	3.7264766804370546
>500	0.00721939060313161	1.8734478653704096
>1k	0.003369048948128084	2.6557527301333943
>5k	9.625854137508812E-4	3.077442581495963
>10k+	0.0014438781206263219	14.063073957870504
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	49406	9.000123873299474	No Hit
GAATCTGTCTCTTATACACATCTGACGCGAGCTCCATCGTATGCCGTCTT	14746	2.6862289324307587	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	12985	2.3654335201148378	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGAGCTCCATCGTATGCC	9912	1.8056355064596283	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGAGCTCCATCGTATG	6968	1.2693369863812236	No Hit
GAACTGTCTCTTATACACATCTGACGCGAGCTCCATCGTATGCCGTCTTC	3787	0.6898649781035726	No Hit
GCTGTCTCTTATACACATCTGACGCGAGCTCCATCGTATGCCGTCTTCTG	2061	0.37544539737825805	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2019	0.36779439946953085	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGAGCTCCATCGTATGCCGT	1912	0.3483025714639638	No Hit
GAATGACTGTCTCTTATACACATCTGACGCGAGCTCCATCGTATGCCGTC	1903	0.34666307191209367	No Hit
GAATGCTGTCTCTTATACACATCTGACGCGAGCTCCATCGTATGCCGTCT	1726	0.3144195807253146	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGAGCTCCA	1159	0.21113110895749687	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGAGCTCCATCGT	985	0.17943411762134118	No Hit
TGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	824	0.15010529230455344	No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCGAGCTCCATCGTATGCCGTC	824	0.15010529230455344	No Hit
GAATGATACCTGGCTCTTATACACATCTGACGCGAGCTCCATCGTATGCC	823	0.149923125687679	No Hit
TGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAAT	809	0.14737279305143655	No Hit
CATTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	796	0.14500462703206862	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCGAGCTCCATCG	696	0.1267879653446228	No Hit
GGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAAT	640	0.11658663479965316	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	636	0.11585796833215532	No Hit
AGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAAT	625	0.11385413554653627	No Hit
CGCTGTCTCTTATACACATCTGACGCGAGCTCCATCGTATGCCGTCTTCT	557	0.10146680559907315	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.11257896922841508	0.0
2	0.0	0.0	0.0	0.703527474369157	0.0
3	0.0	0.0	0.0	0.9040929195479354	0.0
4	0.0	0.0	0.0	1.88906781698813	0.0
5	0.0	0.0	0.0	5.284835722144902	0.0
6	0.0	0.0	0.0	6.057586510926353	0.0
7	0.0	0.0	0.0	6.732695993063095	0.0
8	0.0	0.0	0.0	7.453893629269075	0.0
9	0.0	0.0	0.0	7.684880899465887	0.0
10	3.643332337489161E-4	0.0	0.0	9.90622062563303	0.0
11	3.643332337489161E-4	0.0	0.0	10.133928896726102	0.0
12	3.643332337489161E-4	0.0	0.0	11.701290468313939	0.0
13	3.643332337489161E-4	0.0	0.0	11.82024526913296	0.0
14	3.643332337489161E-4	0.0	0.0	11.927905739705764	0.0
15	3.643332337489161E-4	0.0	0.0	12.167819174129425	0.0
16	3.643332337489161E-4	0.0	0.0	12.367473786223831	0.0
17	3.643332337489161E-4	0.0	0.0	12.45728192834294	0.0
18	3.643332337489161E-4	0.0	0.0	12.533974074047087	0.0
19	3.643332337489161E-4	0.0	0.0	12.82999482646808	0.0
20	5.464998506233741E-4	0.0	0.0	12.884098311679795	0.0
21	5.464998506233741E-4	0.0	0.0	12.928364799580288	0.0
22	5.464998506233741E-4	0.0	0.0	13.027281272543119	0.0
23	7.286664674978322E-4	0.0	0.0	13.085938923176695	0.0
24	7.286664674978322E-4	0.0	0.0	13.148422072764633	0.0
25	7.286664674978322E-4	0.0	0.0	13.185948395840771	0.0
26	7.286664674978322E-4	0.0	0.0	13.242784380305602	0.0
27	7.286664674978322E-4	0.0	0.0	13.330224356405342	0.0
28	7.286664674978322E-4	0.0	0.0	13.375948177240831	0.0
29	7.286664674978322E-4	0.0	0.0	13.423311497628191	0.0
30	7.286664674978322E-4	0.0	0.0	13.477961482690528	0.0
31	9.108330843722903E-4	0.0	0.0	13.523138803675394	0.0
32	9.108330843722903E-4	0.0	0.0	13.570866457296502	0.0
33	9.108330843722903E-4	0.0	0.0	13.629524107930077	0.0
34	0.0010929997012467483	0.0	0.0	13.684902759459913	0.0
35	0.0012751663181212063	0.0	0.0	13.74283174362599	0.0
36	0.0014573329349956644	0.0	0.0	13.784365732273367	0.0
37	0.0014573329349956644	0.0	0.0	13.839380050569453	0.0
38	0.0014573329349956644	0.0	0.0	13.899677200754898	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CACGAGG	20	7.852883E-4	44.000004	2
TATAGCG	20	7.852883E-4	44.000004	1
CCGCAAT	20	7.852883E-4	44.000004	33
TTCGTAG	30	2.5252903E-6	44.0	1
GACGGTC	30	2.5252903E-6	44.0	29
CGGTCTA	30	2.5252903E-6	44.0	31
CGGACGG	30	2.5252903E-6	44.0	2
ATAGCGG	45	4.783942E-10	43.999996	2
TAGCCGT	45	4.783942E-10	43.999996	44
CGTTTTT	15620	0.0	43.507042	1
ATGATCT	225	0.0	42.044445	3
CCGATGA	625	0.0	41.536	18
AATGATC	235	0.0	41.191486	2
GAATCTG	1675	0.0	40.979103	1
CGGCTGT	795	0.0	40.955975	9
CTTCGGA	70	0.0	40.857143	13
ATGCGGG	70	0.0	40.857143	3
ACCGACT	65	0.0	40.615387	17
AATCTGT	1730	0.0	40.566475	2
ACGGCTG	805	0.0	40.44721	8
>>END_MODULE